Probing the surface microstructure of layer-by-layer self-assembly chitosan/poly( l -glutamic acid) multilayers: A grazing-incidence small-angle X-ray scattering study

Nie, Zhaogang; Yang, Chunming; Wang, Yuzhu; Zhao, Binyu; Bian, Fenggang; Li, Xiuhong; Wang, Jie

doi:10.1016/j.msec.2015.08.048

Cited by 14 publications

(10 citation statements)

References 64 publications

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“…Similar topographical features revealed LbL films composed of, e.g. poly(L-lysine) and hyaluronan (Picart et al 2001), chitosan and hyaluronan (Cardoso et al 2016), chitosan and poly(γ-glutamic acid) (Zhao et al 2016). The islets' formation was explained by the aggregation of the polyelectrolytes at a substrate surface during the deposition cycles.…”

Section: Discussionmentioning

confidence: 72%

Protein Interactions With Quaternized Chitosan/Heparin Multilayers

Kumorek

Kubies

Riedel³

2016

Physiol Res

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Understanding the behavior of single proteins at the polyelectrolyte multilayer film/solution interface is of prime importance for the designing of bio-functionalized surface coatings. In the present paper, we study the adsorption of the model proteins, albumin and lysozyme, as well as basic fibroblast growth factor (FGF-2) on a polysaccharide multilayer film composed of quaternized chitosan and heparin. Several analytical methods were used to describe the formation of the polysaccharide film and its interactions with the proteins. Both albumin and lysozyme adsorbed on quaternized chitosan/heparin films, however this process strongly depended on the terminating polysaccharide. Protein adsorption was driven mainly by electrostatic interactions between protein and the terminal layer of the film. The effective binding of FGF-2 by the heparin-terminated film suggested that other interactions could also contribute to the adsorption process. We believe that this FGF-2-presenting polysaccharide film may serve as a biofunctional surface coating for biologically-related applications.

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Section: Discussionmentioning

confidence: 72%

Protein Interactions With Quaternized Chitosan/Heparin Multilayers

Kumorek

Kubies

Riedel³

2016

Physiol Res

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“…This means that the profile of the structure was propagated from the interface, where structure smaller than the critical length was gradually lost. A detailed description can be found in our previous study (Zhao et al, 2016).…”

Section: Results and Application Examplesmentioning

confidence: 99%

SGTools: a suite of tools for processing and analyzing large data sets from in situ X-ray scattering experiments

Nie¹,

Yang²,

Bian³

et al. 2022

J Appl Crystallogr

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In situ synchrotron small-angle X-ray scattering (SAXS) is a powerful tool for studying dynamic processes during material preparation and application. The processing and analysis of large data sets generated from in situ X-ray scattering experiments are often tedious and time consuming. However, data processing software for in situ experiments is relatively rare, especially for grazing-incidence small-angle X-ray scattering (GISAXS). This article presents an open-source software suite (SGTools) to perform data processing and analysis for SAXS and GISAXS experiments. The processing modules in this software include (i) raw data calibration and background correction; (ii) data reduction by multiple methods; (iii) animation generation and intensity mapping for in situ X-ray scattering experiments; and (iv) further data analysis for the sample with an order degree and interface correlation. This article provides the main features and framework of SGTools. The workflow of the software is also elucidated to allow users to develop new features. Three examples are demonstrated to illustrate the use of SGTools for dealing with SAXS and GISAXS data. Finally, the limitations and future features of the software are also discussed.

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“…This is a natural polypeptide, non-toxic, hydrophilic and contain l-glutamic acid which linked by amide bonds, and it is produced by microbial species of Bacillus [145]. The high biodegradability Poly l-glutamic acid [146,147], make it as promising composite in encapsulation [148] and tissue engineering [149].…”

Section: Poly L-glutamic Acidmentioning

confidence: 99%

Biopolymers for Biological Control of Plant Pathogens: Advances in Microencapsulation of Beneficial Microorganisms

et al. 2021

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The use of biofertilizers, including biocontrol agents such as Pseudomonas and Bacillus in agriculture can increase soil characteristics and plant acquisition of nutrients and enhancement the efficiency of manure and mineral fertilizer. Despite the problems that liquid and solid formulations have in maintaining the viability of microbial agents, encapsulation can improve their application with extended shelf-life, and controlled release from formulations. Research into novel formulation methods especially encapsulation techniques has increased in recent years due to the mounting demand for microbial biological control. The application of polymeric materials in agriculture has developed recently as a replacement for traditional materials and considered an improvement in technological processes in the growing of crops. This study aims to overview of types of biopolymers and methods used for encapsulation of living biological control agents, especially microbial organisms.

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Probing the surface microstructure of layer-by-layer self-assembly chitosan/poly( l -glutamic acid) multilayers: A grazing-incidence small-angle X-ray scattering study

Cited by 14 publications

References 64 publications

Protein Interactions With Quaternized Chitosan/Heparin Multilayers

Protein Interactions With Quaternized Chitosan/Heparin Multilayers

SGTools: a suite of tools for processing and analyzing large data sets from in situ X-ray scattering experiments

Biopolymers for Biological Control of Plant Pathogens: Advances in Microencapsulation of Beneficial Microorganisms

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