Probing the yeast 5 S RNA-protein complex by fluorescence and controlled proteolytic digestion

Yeh, Lee Chuan C; Lee, John C.

doi:10.1016/0003-9861(90)90748-n

Cited by 8 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The initial step in complex formation is dependent on co-translational interaction of 5S rRNA with nascent rpL5 (Lin et al 2001). This initial interaction induces structural rearrangements that result in folding of the C-terminal sequence while the Nterminus remains Xexible and accessible for proteolytic cleavage (Yeh and Lee 1990). The structural rearrangements facilitate stable interaction between rpL5 and 5S rRNA.…”

Section: The Importance Of the C-terminal Amino Acidsmentioning

confidence: 99%

Functional features of the C-terminal region of yeast ribosomal protein L5

et al. 2008

View full text Add to dashboard Cite

The aim of this study was to analyze the functional importance of the C-terminus of the essential yeast ribosomal protein L5 (YrpL5). Previous studies have indicated that the C-terminal region of YrpL5 forms an alpha-helix with a positively charged surface that is involved in protein-5S rRNA interaction. Formation of an YrpL5.5S rRNA complex is a prerequisite for nuclear import of YrpL5. Here we have tested the importance of the alpha-helix and the positively charged surface for YrpL5 function in Saccharomyces cerevisiae using site directed mutagenesis in combination with functional complementation. Alterations in the sequence forming the putative alpha-helix affected the functional capacity of YrpL5. However, the effect did not correlate with a decreased ability of the protein to bind to 5S rRNA as all rpL5 mutants tested were imported to the nucleus whether or not the alpha-helix or the positively charged surface were intact. The alterations introduced in the C-terminal sequence affected the growth rate of cells expressing mutant but functional forms of YrpL5. The reduced growth rate was correlated with a reduced ribosomal content per cell indicating that the alterations introduced in the C-terminus interfered with ribosome assembly.

show abstract

Section: The Importance Of the C-terminal Amino Acidsmentioning

confidence: 99%