Probiotic Adhesion to Skin Keratinocytes and Underlying Mechanisms

Lizardo, Mariana V.P.; Magalhães, Rui Miguel; Tavaria, Freni K.

doi:10.3390/biology11091372

Cited by 7 publications

(5 citation statements)

References 40 publications

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“…Probiotics usage including lactobacillus could ameliorate skin conditions suggesting them as a potential co-adjuvant for the treatment of skin infections (15). Previous studies have suggested that probiotic strain extension in the vaginal area could be able to prevent and reduction of HPV infections or expansion the HPV-induced warts (16).…”

Section: Discussionmentioning

confidence: 99%

Using D.SAP, an apple-based formulation for treatment of human papilloma virus, a case report

Dezhakam,

Dezhakam

et al. 2024

Preprint

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The Human papilloma virus (HPV) with almost 1% of prevalence in sexually active adults has more than thirty-five (HPV) types, that can infect the genital tract in women and cause a visible genital HPV infection and genital warts that reveal on the cervix, vagina, urethra, anus, and surrounding areas are mostly caused by HPV genotypes 6, 11 and 18. Moreover, the oncogenic HPV types including 16,18,31,33 and 35 are detected in most of the genital warts and are significantly associated with intraepithelial neoplasia of vulval (VIN), cervical (CIN), and anal (AIN). About fifty percent of the infected group have a subclinical or latent infection. Some of the HPV types can cause cervical, vaginal, and vulvar cancers. The most common treatment methods for removal of the HPV-induced vaginal warts are including cryotherapy, electrocautery, laser therapy, surgery, or trichloroacetic acid. In the present case report the treatment of a 41 years old woman with HPV infection and HPV-induced vaginal warts using an Iranian specially formulated apple-based sap called Dezhakam sap (D.SAP) has been presented. Results showed the disappearance of warts on the vaginal area, and replacement with normal tissue after three months of treatment. D.SAP is an antioxidant and probiotic-rich compound that could help the immune system fight against HPV infections and may present as a potential complementary therapy for HPV-induced warts.

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Section: Discussionmentioning

confidence: 99%

Using D.SAP, an apple-based formulation for treatment of human papilloma virus, a case report

Dezhakam,

Dezhakam

et al. 2024

Preprint

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“…Candida albicans ) [ 57 ]. DSM 20021 strain can reduce the adhesion of pathogenic bacteria [ 58 ]. All these strains contain bacteriocin-related genes and have the ability to inhibit the growth of pathogenic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the anti-pathogenic, genomic and phenotypic properties of a Lacticaseibacillus rhamnosus VHProbi M14 isolate

Zhang¹,

Li²,

Bu³

et al. 2023

PLoS ONE

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A strain of lactic acid bacteria from cheese was isolated, that showed strong growth inhibitory effects on Streptococcus mutans. The API 50CH system and 16S rDNA sequencing verified that this was a novel strain, and was named Lacticaseibacillus rhamnosus VHProbi M14. The strain inhibited the growth of S. mutans and Fusobacterium nucleatum under mixed culture conditions, coaggregated with S. mutans and F. nucleatum, and reduced the adhesion of S. mutans and F. nucleatum on cultured human primary gingival epithelial (HPGE) cells. The pH, peroxidase and protease sensitivity testing found antibacterial substances of protein- and peptide-like structures in addition to organic acids. The antimicrobial substances were sensitive to hydrolysis with trypsin, papain and pineapple protease and were inactived at temperatures above 100°C. Ammonium sulphate-precipitated proteins from the M14 strain retained the ability to inhibit the growth of S. mutans and F. nucleatum. The M14 strain contained 23 bacteriocin-related genes encoding for metabolites, belonging to class II bacteriocins. The M14 strain also showed inhibitory effects on 8 other pathogenic strains (A. actinomycetemcomitans, C. albicans, E. coli, G. vaginalis, P. acnes, P. gingivalis, S. aureus, S. enteritids), and thus has a broad spectrum of bacterial inhibition. This new isolate has been identified as having potential to be used as a probiotic bacterium in clinical applications.

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“…This process disrupted by an imbalanced skin microbiota. Ming et al and Lizardo et al foun probiotics of genus Lactobacillus protected the wound from pathogenic bacteria a anti-inflammatory capabilities [4,12]. In another study, Staphylococcus epidermidis, commensal bacterium, was shown to produce anti-inflammatory lipoteichoic ac The use of probiotics in wound healing is poorly described, especially in animals.…”

Section: Discussionmentioning

confidence: 99%

Topical Probiotic Formulation Promotes Rapid Healing in Dog Keratinocyte Cells: A Promising Approach for Wound Management

Barthe,

Gillot,

Perdigon

et al. 2023

IJMS

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The use of probiotics has gained increasing attention as a strategy for wound healing to decrease microbial resistance to disinfectants and antibiotics. This study aimed to investigate the potential of a non-medicinal topical cocktail of probiotic bacteria (CPB) in promoting wound healing in dogs using in vitro scratch assay. Canine Progenitors Epidermal Keratinocytes (CPEK) were exposed to a prototype product formulated with CPB (PPP), non-formulated CPB, and the vehicle. The viability of CPB and CPEK cells was first evaluated in the co-culture model. Then, wound closure was analyzed over time. The CPB required a minimum concentration of 75 CFU/mL for better viability with CPEK. While the CPEK preserved 100% of their viability when PPP was diluted to up to 75,000 CFU/mL. At higher concentrations, the viability of CPEK was reduced by the concomitant effect of the non-formulated CPB and the vehicle. The formulated and non-formulated CPB and the vehicle seem to lead to a dose-dependent increase in cell migration compared to the control. Importantly, at the concentration of 750,000 CFU/mL, the PPP showed a 20% increase in wound closure. Taken together, our findings suggest the potential beneficial effects of the probiotic-based topical cocktail (PPP) on wound healing. However, to confirm and validate these effects, further experiments are necessary to provide more robust evidence and allow us to confidently establish the potential beneficial effects of the probiotic bacteria (CPB) in promoting wound healing.

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Probiotic Adhesion to Skin Keratinocytes and Underlying Mechanisms

Cited by 7 publications

References 40 publications

Using D.SAP, an apple-based formulation for treatment of human papilloma virus, a case report

Using D.SAP, an apple-based formulation for treatment of human papilloma virus, a case report

Characterization of the anti-pathogenic, genomic and phenotypic properties of a Lacticaseibacillus rhamnosus VHProbi M14 isolate

Topical Probiotic Formulation Promotes Rapid Healing in Dog Keratinocyte Cells: A Promising Approach for Wound Management

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