Probiotic bacteria Lactobacillus rhamnosus influences the blood profile in rainbow trout Oncorhynchus mykiss (Walbaum)

Abstract

This paper reports the effect of feeding probiotic diets on blood profiles in rainbow trout. Two experiments were performed: in the first, fish of average weight 75 g were offered either a commercial feed or the same incorporated with 10(9) CFU g(-1) of lactic acid bacteria Lactobacillus rhamnosus for 30 days; in the second study performed for a similar duration, fish of average weight 126 g were offered formulated diets that either contained the same bacteria in heat-killed or freeze-dried form (nearly 10(11) CFU g(-1)), or the basal diet without the bacteria. Blood samples were collected at different times after commencement of probiotic feeding to determine the total cholesterol, triglyceride contents, the plasma alkaline phosphatase activity, plasma protein and hematocrit value. The plasma cholesterol significantly increased upon probiotic feeding in the first experiment. A significant elevation (P<0.05) of plasma cholesterol and triglyceride and alkaline phosphatase activity level was found in the freeze-dried probiotic fed groups at 20 and 30 days postfeeding. This was concomitant with the increased plasma protein and hematocrit values in FD group at 20 and 30 days. Likewise, the heat-killed probiotic fed group registered significantly high values of triglycerides, alkaline phosphatase activity, and plasma protein compared to the control diet fed groups after 20 days of feeding. Thus, alterations in the blood profiles could serve as supplementary information when examining the benefits of probiotics for fish.

Synbiotic (Biomin imbo) alters gut bacterial microflora of Russian sturgeon, Acipenser guldenstadti (Brandt & Ratzeburg, 1833) in a time-dependent pattern

“…To meet these goals, recently the simultaneous use of probiotics and prebiotics or Synbiotics has obtained special regards in aquaculture. Examinations of Synbiotics on fish have showed that they improve growth parameters by influence on gut microflora, digestion enzymes, appetite and immune system (Irianto and Austin 2002;Merrifield et al 2009;Panigrahi et al 2010;Montajami et al 2012;Nekoubin et al 2012). Nevertheless, rare information are available about the efficacy of Synbiotics on fish in particular sturgeons.…”

Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nda Salvia officinalis ve Aloe vera Ekstraktı ile Beslemenin İmmunolojik ve Antistreptokokal Etkileri

“…Total immunoglobulin was measured by the method of Panigrahi [19] and Bradford [20] . Briefly, the serum samples were diluted 100 times with 0.85% NaCl and the was employed to determining the total Immunoglobulin content, The bovine Plasma albumin (BSA) and reagents being sourced from Sigma (USA).…”

Probiotics use in intensive fish farming

“…In a study carried out by Panigrahi et al (2010), alteration in the blood profile was reported with an increase in plasma cholesterol, triglyceride and alkaline phosphatase activity level after feeding with probiotic strains. The increase in alkaline phosphate activity level is an indicator of good fish health condition (Tukmechi et al, 2007).…”

“…There were alterations of metabolism and/or by the stimulation of host immunity. The strain used for this study enhanced fish growth, feed and nutrients utilization, fish chemical composition and muscular structure, besides fish resistance for pathogenic bacteria (Panigrahi et al, 2010). Another example where a probiotic strain was use to enhance the fish growth and health is in a research paper by Abd El-rhman et al (2009).…”

“…Also, the emergent of antibiotic resistance of human commensal bacteria led to loss of confidence in the use of antibacterial leading to its total ban in European Union (EU) countries in 2006 (Angelis et al, 2006). Probiotics is recognised as an alternative therapy for health management due to restrictions on antibiotics and limitations of vaccinations and chemotherapy (Panigrahi et al, 2010). Its use as farm animal feed supplements such as in pig, cattle and poultry nutrition is from the 1970's, but the concept in aquaculture is a bit new (Tukmechi et al, 2007).…”