Probiotic characterization of bacterial strains from fermented South Indian tomato pickle and country chicken intestine having antioxidative and antiproliferative activities

Abstract

Aim: The present study aims to evaluate the potential antioxidant and antiproliferative properties of probiotic bacterial isolates Weissella cibaria p3B, Bacillus subtilis CS, and Bacillus tequilensis CL, isolated from South Indian fermented tomato pickle (homemade) and gut content of indigenous country chicken. Methods and Results: The bacterial isolates exhibited antimicrobial activity against food-borne, human pathogenic bacteria, along with better survival under different bile and acidic conditions, hydrophobicity towards several hydrocarbons, and adherence to intestinal epithelial cells (INT-407 cells). Also, the intact cell (IC) mixture of the three species showed better DPPH, ABTS, and Fe 2+ chelating activity as compared to the individual IC or cell extract (CE) activity. Among the three bacterial species, W. cibaria p3B revealed maximum antiproliferative activity against HeLa and Caco-2 cancer cells, all of which were nontoxic to INT-407 cells. Apart from being non-hemolytic, the bacterial isolates did not display any necrotic inhibition in HeLa and Caco-2 cells. The cell free supernatant (CFS) of the three bacterial isolates were tested for the production of antimicrobial peptides or bacteriocins. It found that the CFS of bacterial isolates was stable at various temperature, pH and sensitive to proteolytic enzymes confirms protenoius in nature of the antimicrobil peptides or bacteriocins. Conclusion:The bacterial isolates showed promising antimicrobial, antioxidant as well as antiproliferative activities with better survival ability at different pH and bile concentrations. The three bacterial isolates were able to produce potential antimicrobial peptides or bacteriocins. Significance and Impact of the study: These results indicate better compatibility of our bacterial isolates against synthetic drugs to avoid adverse side effects and can be processed as dietary supplements against food and human pathogens. They can also provide antioxidative and antiproliferative benefits to humans and animals.

Bacillus subtilis-Based Probiotic Improves Skeletal Health and Immunity in Broiler Chickens Exposed to Heat Stress

“…Similarly, Bacillus subtilis has been used as a probiotic with multiple functions in poultry [ 141 ]. As a growth promoter, for example, Bacillus subtilis has been demonstrated to improve chickens’ growth performance [ 142 , 143 ]; regulate intestinal microstructure [ 144 ] and digestive enzymes [ 145 , 146 ]; synthesize and release antimicrobial and antibiotic compounds [ 147 , 148 , 149 , 150 ]; increase immunity against gut inflammation and infectious diseases [ 151 , 152 ]; and enhance neurochemical activities [ 153 , 154 ] via the bidirectional communication between the gut and brain, regulating stress responses under various rearing conditions [ 155 , 156 , 157 ].…”

Feasible mechanisms and therapeutic potential of food probiotics to mitigate diabetes‐associated cancers: A comprehensive review and in silico validation

“…A consortium of B. subtilis CS + B. tequilensis CL + W. cibaria p3B revealed effective antiproliferative properties in food, feed, and dairy industries (Ankaiah et al, 2021). Similar studies have proved that L.…”

“…Thus, functional foods and preparative probiotics can have potent implications in dietary supplementation and augmented bioactivities. A consortium of B. subtilis CS + B. tequilensis CL + W. cibaria p3B revealed effective antiproliferative properties in food, feed, and dairy industries (Ankaiah et al., 2021). Similar studies have proved that L. acidophilus positively affects colorectal cancer Caco‐2 cell lines, suggesting survival longevity (Isazadeh et al., 2020).…”

The Weissella Genus: Clinically Treatable Bacteria with Antimicrobial/Probiotic Effects on Inflammation and Cancer

“…A review by Kwak et al [114] investigated the benefits of kimchi LAB (W. cibaria and L. plantarum) against cancer and derived the same conclusion about W. cibaria [114]. The anti-proliferative activity of W. cibaria against cancer cells such as HeLa and Caco-2 has been confirmed in other studies as well [112,115,116]. A unique single-center study by Kwon et al investigated the role of probiotic usage in post-operative cancer patients and noticed an increase in the populations of several beneficial bacteria that also included a very significant increase in the Weissella bacteria (a rise from 0.096% to 0.361%, p < 0.004) after 8 weeks of probiotic supplementation.…”