Probiotic Effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on Hypercholesteremic Golden Hamsters

Abstract

Hypercholesteremia or high cholesterol is one of the important factors leading to atherosclerosis and other cardiovascular diseases. The application of probiotics with cholesterol-lowering characteristics has become increasingly popular over the past decade due to their contribution to human health. This study aimed to evaluate the probiotic effects of Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 on hyperlipidemic golden hamsters. A hyperlipidemic model was established through a high cholesterol diet in golden hamsters, after which lyophilized Lactobacillus fermentum ZJUIDS06 and Lactobacillus plantarum ZY08 were orally administered individually for 8 weeks. The physiological characteristics of golden hamsters and short chain fatty acid (SCFA) in the colon were assessed by automatic Biochemical Analyzer and gas choromatograph, respectively. A MiSeq sequencing-based analysis of the bacterial 16S rRNA gene (V3–V4 region) in the cecum content was performed to analyze the cecum microbiota. Correlations between sets of these variables were also investigated using the R package “corrplot.” Results showed that neither Lactobacillus fermentum ZJUIDS06 nor Lactobacillus plantarum ZY08 inhibited body weight increase. However, supplementation with Lactobacillus fermentum ZJUIDS06 for 8 weeks increased colon SCFA levels (P < 0.05), decreased serum low-density lipoprotein, total cholesterol, and triglycerides levels, and also induced changes in the cecum microbiota of hyperlipidemic golden hamsters. Remarkably, oral administration of Lactobacillus fermentum ZJUIDS06 increased the relative abundance of Parabacteroides in the cecum, which served as a biomarker for colon SCFA production and improvement of serum cholesterol levels. In a word, Lactobacillus fermentum ZJUIDS06 improved hyperlipidemia in golden hamsters, which correlated with an increase in SCFA levels and relative abundance of Parabacteroides, indicating its potential importance in functional foods that can help lower cholesterol.

Lactiplantibacillus plantarum J‐15 reduced calcium oxalate kidney stones by regulating intestinal microbiota, metabolism, and inflammation in rats

“…As a probiotic, J‐15 mainly plays a role in regulating intestinal flora affected by kidney stone formation and helped restore the intestinal flora. This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments 54–56 . Our previous study on the microbial community composition of different intestinal segments after L .…”

“…This is pretty consistent with previous investigation on the changes in intestinal or fecal abundance of genus Lactobacillus after the administration of Lactobacillus strains in other disease models, 52,53 which might be due to the strain specificity, dosage, different animal models, and intestinal segments. [54][55][56] Our previous study on the microbial community composition of different intestinal segments after L. plantarum administration revealed that the amount of genus Lactobacillus varied widely among intestinal segments, 57 with the higher levels in the ileum and less in the cecum and colon. It suggests that L. plantarum significantly regulates the intestinal microflora of the ileum, rather than the cecum and colon.…”

The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats

“…Each strain of the Lab4 consortium possesses gene sequences encoding phosphate acetyltransferase and acetate kinase that are involved in the generation of acetate ( 31 ): acetate is recognised as an anti-inflammatory SCFA known to inhibit the expression of IL-6 by colon cells ( 32 ). The recent study of Yang et al showed that administration of Lactobacillus fermentum ZJUIDS06 increased levels of acetic, propionic and butyric acid in the colons of hypercholesterolemic hamsters whilst significantly reducing plasma cholesterol levels ( 33 ). SCFA are known to have pleiotropic effects on the host and there is growing evidence supporting beneficial effects on cholesterol metabolism ( 34 ).…”

Analysis and Validation of Hub Genes in Blood Monocytes of Postmenopausal Osteoporosis Patients

“…Also, genes that are not statistically different are indicated by a cross. In this analysis, Package “corrplot” was used to visualize the results ( 29 , 30 ).…”