Probiotic effects of β-glucuronidase on the peach-potato aphid Myzus persicae (Aphididae)

Cherqui, Anas; Alla, Salah; Saguez, Julien; Doury, Géraldine; Sangwan‐Norreel, B. S.; Giordanengo, Philippe

doi:10.1016/j.jinsphys.2003.09.004

Cited by 16 publications

(13 citation statements)

References 66 publications

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“…A standard diet adapted for M. persicae was used as a control and as a basis for the protein dilution. The concentration of the proteins added to the control diet were chosen according to Cherqui et al (2003) and Couty et al (2002). The diet was prepared as described by Febvay et al (1988) and modified by Down et al (1996).…”

Section: Artificial Dietsmentioning

confidence: 99%

Unexpected effects of chitinases on the peach-potato aphid (Myzus persicae Sulzer) when delivered via transgenic potato plants (Solanum tuberosum Linn�) and in vitro

et al. 2005

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With the aim of producing insect-resistant potato plants, internode explants of Solanum tuberosum L. cv. Désirée were transformed with an Agrobacterium strain C58pMP90 containing an insect (Phaedon cochleariae: Coleoptera, Chrysomelidae) chitinase gene and the neomycin phosphotransferase (nptII) gene as selectable marker, both under the control of the viral CaMV 35S promoter. Three transformed potato lines (CH3, CH5 and CH25) exhibiting the highest chitinolytic activities were selected for feeding experiments with the peach-potato aphid, Myzus persicae (Sulzer), under controlled photoperiod and temperature conditions. Aphids fed on transgenic potato plants showed a reduced pre-reproductive period and an enhanced daily fecundity. Transgenic potato lines did not affect nymphal mortality, but improved several biological parameters related to aphid population's growth. Artificial diets were used to provide active (1, 10, 100 and 500 microg ml(-1)) and inactive (500 microg ml(-1)) bacterial (Serratia marcescens) chitinase to M. persicae. These compounds increased nymph survival at all active chitinase doses when compared to the control diet, while inactive chitinase did not. Although the pre-reproductive period was slightly shortened and the daily fecundity slightly higher, active and inactive chitinase provided as food led a reduction from 1 to 1.5 day population's doubling time. Therefore chitinase activity was responsible for the probiotic effects on aphids. Our results question the relevance of a chitinase-based strategy in the context of potato culture protection.

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Section: Artificial Dietsmentioning

confidence: 99%

Unexpected effects of chitinases on the peach-potato aphid (Myzus persicae Sulzer) when delivered via transgenic potato plants (Solanum tuberosum Linn�) and in vitro

et al. 2005

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“…However, recent studies reveal putative protease activity in aphids like Myzus persicae [49], [50] and Aphis gossypii [51]. These revelations suggest presence of corresponding enzyme inhibitors in the plants as plant-insect defense reaction is the result of an evolutionary arms race.…”

Section: Resultsmentioning

confidence: 99%

Identification of Genes Involved in Wild Crucifer Rorippa indica Resistance Response on Mustard Aphid Lipaphis erysimi Challenge

2013

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Mustard aphid, Lipaphis erysimi (L.) Kaltenbach is a perpetual annual threat in the cultivation of rapeseed- mustard (Brassica spp.) crop in tropical and sub-tropical climate. Cultivated Brassica germplasm has failed so far to provide any source of resistance. Wild germplasm is a potential source of resistance against many threatening herbivores. On wild germplasm screening, we noted that the wild crucifer Rorippa indica (L.) Hiern confers resistance against L. erysimi. In the present study L. erysimi challenged transcriptome of R. indica was compared to un-infested R. indica sample to get a molecular insight about the aphid resistance mechanism and identify the candidate defense response genes. Cloning, sequencing and in silico sequence analysis of complimentary DNA amplified fragment length polymorphism identified 116 differentially expressed transcript derived fragments revealed thirty candidates which are from different functional categories including redox regulation, signalling, photosynthesis, structure, metabolism, defense response as well as a few of unknown function. Twenty four identifications were then studied by quantitative real time RT PCR analysis at 6, 12, 24 and 48 hour time point post infestation to understand the early-to-late defense response through their relative gene expression profiles. Seventeen fragments showed significant up or down regulation at p<0.05 level. The response was influenced by different phytohormonal signalling pathways simultaneously. The candidate defense response expressed sequence tags specifically for the resistance genes identified in this study have implication in building desired mustard aphid resistance in susceptible rapeseed-mustard plants in future. This is the first molecular report on crucifer defense response against mustard aphid L. erysimi.

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“…The proteins oryzacystatin , b-glucoronidase (Cherqui et al, 2003), and snowdrop lectin (GNA) (Down et al, 2000) have also been observed to pass through the gut epithelium of aphids. Assuming Manse-AS exerts its effects through interactions with receptors in the alimentary canal to inhibit gut motility, then when fed to M. persicae, the intact peptide, or an active fragment, created by enzyme metabolism, must have traversed the gut epithelium.…”

Section: Discussionmentioning

confidence: 99%

Effects of Manduca sexta allatostatin and an analogue on the peach‐potato aphid Myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut

Matthews¹,

Down²,

Audsley³

2010

Arch Insect Biochem Physiol

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The oral toxicity of the C-type allatostatin, Manduca sexta allatostatin (Manse-AS) and the analogue δR³δR⁵Manse-AS, where R residues were replaced by their D-isomers, were tested against the peach-potato aphid Myzus persicae by incorporation into an artificial diet. Both peptides had significant dose-dependent effects on mortality, growth, and fecundity compared with control insects. The analogue, δR³δR⁵Manse-AS, had an estimated LC₅₀ of 0.31 µg/µl diet and was more potent than Manse-AS (estimated LC₅₀ of 0.58 µg/µl diet). At a dose of 0.35 µg δR³δR⁵Manse-AS/µl diet, 76% of the aphids were dead after 6 days and all were dead after 10 days. In comparison, three times the dose of Manse-AS was required to achieve 74% mortality after 8 days and 98% mortality after 16 days. The degradation of both peptides by extracts prepared from the gut of M. persicae was investigated. The estimated half-life of Manse-AS, when incubated with the gut extract from M. persicae, was 31 min. Degradation was due to a cathepsin L-like cysteine protease, carboxypeptidase-like activity, endoprotease activity with glutamine specificity, pyroglutamate aminopeptidase activity, and possibly trypsin-like proteases. The half-life of the δR³δR⁵ Manse-AS analogue was enhanced (73 min) with the D-isomers of R appearing to prevent cleavage around the R residues by cathepsin L-like cysteine proteases or from trypsin-like proteases. The greater stability of the analogue may explain its increased potency in M. persicae. This work demonstrates the potential use of Manse-AS and analogues, with greater resistance to enzymatic attack, in aphid control strategies.

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Probiotic effects of β-glucuronidase on the peach-potato aphid Myzus persicae (Aphididae)

Cited by 16 publications

References 66 publications

Unexpected effects of chitinases on the peach-potato aphid (Myzus persicae Sulzer) when delivered via transgenic potato plants (Solanum tuberosum Linn�) and in vitro

Unexpected effects of chitinases on the peach-potato aphid (Myzus persicae Sulzer) when delivered via transgenic potato plants (Solanum tuberosum Linn�) and in vitro

Identification of Genes Involved in Wild Crucifer Rorippa indica Resistance Response on Mustard Aphid Lipaphis erysimi Challenge

Effects of Manduca sexta allatostatin and an analogue on the peach‐potato aphid Myzus persicae (hemiptera: aphididae) and degradation by enzymes in the aphid gut

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