Probiotic
            <i>Lactobacillus reuteri</i>
            Ameliorates Disease Due to Enterohemorrhagic
            <i>Escherichia coli</i>
            in Germfree Mice

Eaton, Kathryn A.; Honkala, Alexander; Auchtung, Thomas A.; Britton, Robert A.

doi:10.1128/iai.00880-10

Cited by 63 publications

(29 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed that Helicobacter abundance increased in late summer/fall, when Lactobacillus levels are low. This might suggest that Lactobacillus confers protection against infection as has been demonstrated in laboratory mice ( Kabir et al, 1997 ; Pena et al, 2005 ; Medellin-Pena and Griffiths, 2009 ; Eaton et al, 2011 ). Alternatively, immune status (that is, IL-22 deficiency) has been linked to the abundance of Lactobacillus ( Zenewicz et al, 2013 ), potentially suggesting that these seasonal changes might be in part driven by the host response to bacterial infection.…”

Section: Discussionmentioning

confidence: 66%

Marked seasonal variation in the wild mouse gut microbiota

et al. 2015

View full text Add to dashboard Cite

Recent studies have provided an unprecedented view of the microbial communities colonizing captive mice; yet the host and environmental factors that shape the rodent gut microbiota in their natural habitat remain largely unexplored. Here, we present results from a 2-year 16 S ribosomal RNA gene sequencing-based survey of wild wood mice (Apodemus sylvaticus) in two nearby woodlands. Similar to other mammals, wild mice were colonized by 10 bacterial phyla and dominated by the Firmicutes, Bacteroidetes and Proteobacteria. Within the Firmicutes, the Lactobacillus genus was most abundant. Putative bacterial pathogens were widespread and often abundant members of the wild mouse gut microbiota. Among a suite of extrinsic (environmental) and intrinsic (host-related) factors examined, seasonal changes dominated in driving qualitative and quantitative differences in the gut microbiota. In both years examined, we observed a strong seasonal shift in gut microbial community structure, potentially due to the transition from an insect- to a seed-based diet. This involved decreased levels of Lactobacillus, and increased levels of Alistipes (Bacteroidetes phylum) and Helicobacter. We also detected more subtle but statistically significant associations between the gut microbiota and biogeography, sex, reproductive status and co-colonization with enteric nematodes. These results suggest that environmental factors have a major role in shaping temporal variations in microbial community structure within natural populations.

show abstract

Section: Discussionmentioning

confidence: 66%

Marked seasonal variation in the wild mouse gut microbiota

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Furthermore, the genes cyclopropane fatty acid synthase (cfa) [43] and L. reuteri-specific immunoregulatory (rsiR) gene [44] have been implicated in the anti-inflammatory properties of L. reuteri 6475. In addition to these immunomodulatory properties in vitro, L. reuteri 6475 has also shown promising effects in animal studies [45][46][47].…”

Section: Immunomodulatory Effects Of Selected Lactic Acid Bacteriamentioning

confidence: 96%

Immunomodulation of Monocytes by Probiotic and Selected Lactic Acid Bacteria

Jensen

Drømtorp

Axelsson

et al. 2014

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Some lactic acid bacteria (LAB), especially bacteria belonging to the genus Lactobacillus, are recognized as common inhabitants of the human gastrointestinal tract and have received considerable attention in the last decades due to their postulated health-promoting effects. LAB and probiotic bacteria can modulate the host immune response. However, much is unknown about the mediators and mechanisms responsible for their immunological effect. Here, we present a study using cytokine secretion from the monocytic cell line THP-1 and NF-κB activation in the monocytic cell line U937-3xkB-LUC to elucidate immune stimulating abilities of LAB in vitro. In this study, we investigate both commercially available and potential probiotic LAB strains, and the role of putative surface proteins of L. reuteri using mutants. L. reuteri strains induced the highest cytokine secretion and the highest NF-κB activation, whereas L. plantarum strains and L. rhamnosus GG were low inducers/activators. One of the putative L. reuteri surface proteins, Hmpref0536_10802, appeared to be of importance for the stimulation of THP-1 cells and the activation of NF-κB in U937-3xkB-LUC cells. Live and UV-inactivated preparations resulted in different responses for two of the strains investigated. Our results add to the complexity in the interaction between LAB and human cells and suggest the possible involvement of secreted pro- and anti-inflammatory mediators of LAB. It is likely that it is the sum of bacterial surface proteins and bacterial metabolites and/or secreted proteins that induce cytokine secretion in THP-1 cells and activate NF-κB in U937-3xkB-LUC cells in this study.

show abstract

“…At the gastrointestinal mucosal surface, the presence of lactobacilli is associated with downregulation of immune response to inflammatory stimuli, 20,21 but a similar process has not been defined in the genital tract. Because most of the adverse health outcomes associated with BV are presumably related to inflammatory complications of BV, understanding whether the presence of (or introduction of) Lactobacillus species could mitigate those complications is important.…”

Section: What Is a Healthy Vagina?mentioning

confidence: 99%

Bacterial Vaginosis and the Cervicovaginal Immune Response

Mitchell

Marrazzo

2014

American J Rep Immunol

164

139

View full text Add to dashboard Cite

Bacterial vaginosis (BV) is a common cause of vaginal discharge in reproductive age women around the world, and is associated with several poor reproductive health outcomes, including HIV-1 acquisition. One possible mechanism for this association is the inflammatory immune response induced by BV in the cervical and vaginal mucosae. There is significant heterogeneity in reports of markers of cervicovaginal inflammation in women with bacterial vaginosis, likely due to microbial and host diversity, as well as differences in study design. In this article we review the characteristics of the mucosal immune response in BV, the potential role of lactobacilli in modulating that response, and the impact of individual BV-associated bacterial species on mucosal immunity. We focus on inflammatory markers that are proposed to increase the risk of HIV-1 acquisition.

show abstract

Probiotic Lactobacillus reuteri Ameliorates Disease Due to Enterohemorrhagic Escherichia coli in Germfree Mice

Cited by 63 publications

References 36 publications

Marked seasonal variation in the wild mouse gut microbiota

Marked seasonal variation in the wild mouse gut microbiota

Immunomodulation of Monocytes by Probiotic and Selected Lactic Acid Bacteria

Bacterial Vaginosis and the Cervicovaginal Immune Response

Contact Info

Product

Resources

About