Probiotic <i><scp>L</scp>actobacillus rhamnosus </i><scp>GG</scp> (<scp>LGG</scp>) and prebiotic prevent neonatal inflammation‐induced visceral hypersensitivity in adult rats

Kannampalli, Pradeep; Pochiraju, S.; Chichlowski, Maciej; Berg, Brian M.; Rudolph, Colin; Bruckert, Mitchell; Miranda, Adrian; Sengupta, Jyoti N.

doi:10.1111/nmo.12450

Cited by 56 publications

(35 citation statements)

References 54 publications

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“…In keeping with previously published work from our own laboratory, we saw an increased visceral sensitivity in MS rats (Felice et al., ; O'Mahony et al., ), which was improved with the consumption of MFGM. We and others have previously shown that dietary interventions targeting the gut microbiota reduce visceral sensitivity in several different animal models (Kannampalli et al., ; Larauche, Mulak, Yuan, Kanauchi, & Tache, ; McVey Neufeld et al., ; Wang et al., ). Yet here while we do not see striking differences in the microbiota profile of the control diet‐fed MS and NS rats, the MS rats do display a significant increase in visceral sensitivity.…”

Section: Discussionmentioning

confidence: 92%

“…Notably, this is the first study to show that MFGM consumption results in a significant reduction of the early-life stress-induced visceral hypersensitivity. While previous studies have shown that dietary supplementation with probiotics and prebiotics is effective in the reduction of visceral sensitivity (Kannampalli et al, 2014;McKernan, Fitzgerald, Dinan, & Cryan, 2010;McVey Neufeld et al, 2017;Wang et al, 2017), the impact of MFGM has not been shown before.…”

Section: Discussionmentioning

confidence: 97%

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The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

O’Mahony

Neufeld

Waworuntu

et al. 2019

Eur J of Neuroscience

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Nutritional interventions targeting the microbiota–gut–brain axis are proposed to modulate stress‐induced dysfunction of physiological processes and brain development. Maternal separation (MS) in rats induces long‐term alterations to behaviour, pain responses, gut microbiome and brain neurochemistry. In this study, the effects of dietary interventions (milk fat globule membrane [MFGM] and a polydextrose/galacto‐oligosaccharide prebiotic blend) were evaluated. Diets were provided from postnatal day 21 to both non‐separated and MS offspring. Spatial memory, visceral sensitivity and stress reactivity were assessed in adulthood. Gene transcripts associated with cognition and stress and the caecal microbiota composition were analysed. MS‐induced visceral hypersensitivity was ameliorated by MFGM and to greater extent with the combination of MFGM and prebiotic blend. Furthermore, spatial learning and memory were improved by prebiotics and MFGM alone and with the combination. The prebiotic blend and the combination of the prebiotics and MFGM appeared to facilitate return to baseline with regard to HPA axis response to the restraint stress, which can be beneficial in times where coping mechanisms to stressful events are required. Interestingly, the combination of MFGM and prebiotic reduced the long‐term impact of MS on a marker of myelination in the prefrontal cortex. MS affected the microbiota at family level only, while MFGM, the prebiotic blend and the combination influenced abundance at family and genus level as well as influencing beta‐diversity levels. In conclusion, intervention with MFGM and prebiotic blend significantly impacted the composition of the microbiota as well as ameliorating some of the long‐term effects of early‐life stress.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 97%

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

O’Mahony

Neufeld

Waworuntu

et al. 2019

Eur J of Neuroscience

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“…57 Interestingly, vancomycin treatment did not impact cognitive or anxiety-related behaviors, growth or other parameters in the adult animals. Recently, Kannampali and colleagues 58 used the rat chronic visceral hypersensitivity model to test the effect of probiotics and prebiotics on ameliorating the severity of neonatal stress-induced hypersensitivity.…”

Section: Altered M Icrobiome During Early Life Critical Sensitive Permentioning

confidence: 99%

Visceral Pain and Gastrointestinal Microbiome

Chichlowski

Rudolph

2015

J Neurogastroenterol Motil

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A complex set of interactions between the microbiome, gut and brain modulate responses to visceral pain. These interactions occur at the level of the gastrointestinal mucosa, and via local neural, endocrine or immune activity; as well as by the production of factors transported through the circulatory system, like bacterial metabolites or hormones. Various psychological, infectious and other stressors can disrupt this harmonious relationship and alter both the microbiome and visceral pain responses. There are critical sensitive periods that can impact visceral pain responses in adulthood. In this review we provide a brief background of the intestinal microbiome and emerging concepts of the bidirectional interactions between the microbiome, gut and brain. We also discuss recent work in animal models, and human clinical trials using prebiotics and probiotics that alter the microbiome with resultant alterations in visceral pain responses.

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“…The previous studies had the various recovery duration between 0 to 7 days after EMG surgery. [32][33][34][35][36][37] We allowed the rats recovery duration after surgery to be at least 2 days. EMG surgery might induce local inflammation and there is a possibility that inflammation increases VMR for colorectal distention.…”

Section: Cgrpmentioning

confidence: 99%

Dai-Kenchu-To, a Herbal Medicine, Attenuates Colorectal Distention-induced Visceromotor Responses in Rats

Nakaya

Nagura

Hasegawa

et al. 2016

J Neurogastroenterol Motil

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Background/AimsDai-kenchu-to (DKT), a traditional Japanese herbal medicine, is known to increase gastrointestinal motility and improve ileal function. We tested our hypotheses that (1) pretreatment with DKT would block the colorectal distention-induced visceromotor response in rats, and (2) pretreatment with DKT would attenuate colorectal distention-induced adrenocorticotropic hormone (ACTH) release and anxiety-related behavior. MethodsRats were pretreated with vehicle or DKT (300 mg/kg/5 mL, per os). Visceromotor responses were analyzed using electromyography in response to colorectal distention (10,20, 40, 60, and 80 mmHg for 20 seconds at 3-minutes intervals). Anxiety-related behavior was measured during exposure to an elevated-plus maze after colorectal distention. Plasma ACTH and serum corticosterone levels were measured after exposure to the elevated-plus maze. ResultsColorectal distention produced robust contractions of the abdominal musculature, graded according to stimulus intensity, in vehicletreated rats. At 40, 60, and 80 mmHg of colorectal distention, the visceromotor responses of DKT-treated rats was significantly lower than that of vehicle-treated rats. At 80 mmHg, the amplitude was suppressed to approximately one-third in DKT-treated rats, compared with that in vehicle-treated rats. Smooth muscle compliance and the velocity of accommodation to 60 mmHg of stretching did not significantly differ between the vehicle-treated and DKT-treated rats. Similarly, the DKT did not influence colorectal distentioninduced ACTH release, corticosterone levels, or anxiety-related behavior in rats. ConclusionsOur results suggest that DKT attenuates the colorectal distention-induced visceromotor responses, without increasing smooth muscle compliance, ACTH release or anxiety-related behavior in rats.

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Probiotic Lactobacillus rhamnosus GG (LGG) and prebiotic prevent neonatal inflammation‐induced visceral hypersensitivity in adult rats

Abstract: Results document that in rats LGG can attenuate neonatally induced chronic visceral pain measured in adulthood. Prolonged intake of LGG alters some key brain neurotransmitters and biogenic amines that could be involved in pain modulation.

Cited by 56 publications

References 54 publications

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

The enduring effects of early‐life stress on the microbiota–gut–brain axis are buffered by dietary supplementation with milk fat globule membrane and a prebiotic blend

Visceral Pain and Gastrointestinal Microbiome

Dai-Kenchu-To, a Herbal Medicine, Attenuates Colorectal Distention-induced Visceromotor Responses in Rats

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