Probiotic potential of &lt;i&gt;Lactobacillus&lt;/i&gt; isolates of chicken origin with anti-&lt;i&gt;Campylobacter&lt;/i&gt; activity

Dec, Marta; Nowaczek, Anna; Urban‐Chmiel, Renata; Stępień–Pyśniak, Dagmara; Wernicki, Andrzej

doi:10.1292/jvms.18-0092

Cited by 31 publications

(37 citation statements)

References 32 publications

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“…and assign them to two species: C. jejuni and C. coli . The positive results of identification using MALDI‐TOF mass spectrometry, as well as results obtained by other authors, confirm that these diagnostic techniques can be used to identify this type of pathogen (Bessede, Solecki, Sifré, Labadi, & Mégraud, ; Martiny, Visscher, Catry, Chatellier, & Vandenberg, ) and other microbial isolates from birds (Dec, Nowaczek, Urban‐Chmiel, Stępień‐Pyśniak, & Wernicki, ; Stępień‐Pyśniak, Hauschild, Różański, & Marek, ).…”

Section: Discussionsupporting

confidence: 75%

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

et al. 2019

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Bacteria of the genus Campylobacter are the most common pathogens causing zoonotic diseases in humans. Therefore, the aim of the study was to isolate Campylobacter bacteria from broiler chickens and evaluate their susceptibility to selected antibiotics by determining minimum inhibitory concentrations (MIC), followed by isolation and characterization of bacteriophages specific for Campylobacter spp. The material for the study consisted of field isolates of Campylobacter spp. obtained from the gut (cecum) of broiler chickens directly after slaughter in slaughterhouses, and bacteriophages specific for these strains. We isolated 48 strains from poultry (140 broiler chickens): 31 strains of Campylobacter jejuni and 17 of Campylobacter coli. Identification of the strains was confirmed by multiplex PCR and MALDI‐TOF mass spectrometry. Over 83% of Campylobacter strains were resistant to ciprofloxacin, and over half the isolates were resistant to erythromycin, gentamicin, and tetracycline. Resistance to three or more antibiotics was observed in 91.6% of all strains. Four bacteriophages were obtained, and on the basis of their morphological structure, they were assigned to two families of the order Caudovirales : Myoviridae and Siphoviridae . A high percentage of the Campylobacter strains were resistant to at least three of the antibiotic groups tested. All of the phages exhibited lytic activity against the Campylobacter spp. isolates, but the antibacterial effect of the phages was not observed for all strains.

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Section: Discussionsupporting

confidence: 75%

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

et al. 2019

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“…We also identified several Lactobacillli which have previously been isolated from the chicken gastrointestinal tract and have been suggested as potential probiotics in chickens, including 5 novel strains of Lactobacillus crispatus (62–64), 2 novel strains of Lactobacillus gallinarum (65), a novel strain of Lactobacillus johnsonii (66, 67), a novel strain of Lactobacillus oris (68), a novel strain of Lactobacillus reuteri (59, 62, 69) and a novel strain of Lactobacillus salivarius (59, 67, 70).…”

Section: Resultsmentioning

confidence: 99%

“…Our comparison to chicken faeces samples from eight countries which were part of a pan-EU project on AMR demonstrates that our MAGs are abundant in chicken populations across Europe, and that these new genomes can account for the majority of reads in chicken gut microbiome studies. We also identified several novel Lactobacillus strains which have previously been posited as potential chicken probiotics, including L.crispatus (66–68), L.gallinarum (69), L.johnsonii (70, 71), L.oris (72), L.reuteri (63, 66, 73) and L.salivarius (63, 71, 74).…”

Section: Discussionmentioning

confidence: 99%

“…However, we do think it is likely that there is a core chicken cecal microbiota which is shared across sites and is irrespective of management conditions having assembled bacterial species that have been found in chickens in previous studies (14, 19, 30, 60). We also identified several novel Lactobacillus strains which have previously been posited as potential chicken probiotics, including L.crispatus (62–64), L.gallinarum (65), L.johnsonii (66, 67), L.oris (68), L.reuteri (59, 62, 69) and L.salivarius (59, 67, 70).…”

Section: Discussionmentioning

confidence: 99%

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Assembly of hundreds of novel bacterial genomes from the chicken caecum

Glendinning

Stewart

Pallen

et al. 2019

Preprint

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9Chickens are a highly important source of protein for a large proportion of the human population. The 10 caecal microbiota plays a crucial role in chicken nutrition through the production of short chain fatty acids, 11 nitrogen recycling and amino acid production. In this study we sequenced DNA from caecal contents 12 samples taken from 24 chickens belonging to either a fast or slower growing breed consuming either a 13 vegetable-only diet or a diet containing fish meal. We utilised 1.6T of Illumina data to construct 469 draft 14 metagenome-assembled bacterial genomes, including 460 novel strains, 283 novel species and 42 novel 15 genera. We compared our genomes to data from eight EU countries and show that these genomes are 16 abundant within European chicken flocks. We also compared the abundance of our genomes, and the 17 carbohydrate active enzymes they produce, between our chicken groups and demonstrate that there are 18 both breed-and diet-specific microbiomes, as well as an overlapping core microbiome. This data will form 19 the basis for future studies examining the composition and function of the chicken caecal microbiota. 20 21 Background: 22 157 Data availability 158 Paired-read fastq files have been submitted to the European Nucleotide Archive under project PRJEB33338. 159 MAG fasta files have been submitted to Edinburgh DataShare (https://doi.org/10.7488/ds/2584). 160

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“…Notably, strains such as Lactobacillus spp., i.e., acidophilus, casei, crispatus, gasseri, helveticus, pentosus, plantarum, rhamnosus, and salivarius have been suggested to exhibit anti-Campylobacter activities in vitro and in vivo [49]. In addition, Dec et al [52], in screening Lactobacillus isolates for anti-Campylobacter activity, selected seven Lactobacillus isolates with potential applications in reducing Campylobacter spp. in chickens, which may have potential to prevent infections in both birds and humans [52].…”

Section: Prebiotic Effects Of Gmomentioning

confidence: 99%

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Quinn

Slattery

Walsh

et al. 2020

Foods

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Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3 -sialyllactose, 6 -sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk. Foods 2020, 9, 348 2 of 15Listeria monocytogenes, and Clostridium difficile [4][5][6]. Microbe-associated molecular patterns (MAMPs) are recognized by the host s intestinal pattern recognition receptors (PRRs), and these interactions play key roles in the association of pathogens with the intestinal epithelia. Probiotics also express molecular patterns which can recognize the same trans-membrane receptors as the pathogens, thus blocking the sites for pathogenic contact by competitive exclusion and, in some cases, displacing already-attached pathogens [7]. However, the health benefits associated with bifidobacteria are reliant on such strains colonising the host in sufficient numbers [8]. The important step in microbial colonisation of the intestinal epithelium is the attachment of bacterial surface lectins to intestinal sugar structures. Recent studies have suggested that milk oligosaccharides may enhance the specific ability of bifidobacteria to attach to the GI epithelium [9][10][11]. Indeed, our group investigated the ability of goat milk oligosaccharides (GMO) to increase the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 cells. Exposure of the strain ...

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Probiotic potential of <i>Lactobacillus</i> isolates of chicken origin with anti-<i>Campylobacter</i> activity

Cited by 31 publications

References 32 publications

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

Campylobacter spp. and bacteriophages from broiler chickens: Characterization of antibiotic susceptibility profiles and lytic bacteriophages

Assembly of hundreds of novel bacterial genomes from the chicken caecum

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

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