Probiotic Properties and Antioxidant Efficiency of Lactobacillus Plantarum 15 Isolated From Milk

Abstract

Many researchers are focusing on the investigation of new molecules and substances to help the body fighting oxidative stress; among them are probiotic bacteria. In this study we investigated the probiotic properties and the antioxidative activity of Lactobacillus plantarum 15 isolated from milk. Therefore, to evaluate probiotic potential of the selected strain, the antibacterial activity and resistance to acidic pH and bile salts conditions were evaluated. Moreover, several methods were used to analyze the antioxidant potential of this strain. The evaluation of probiotic properties showed that Lb. plantarum 15 resists to acidity and to bile salts (survival rate was 86.40% and 80.58%, respectively). The isolate showed a broad inhibitory activity against several bacterial strains. The antioxidant potential assessed by the DPPH method showed that Lb. plantarum 15 cells as well as the cell-free supernatant have important scavenger effect (82.65% and 72.21%, respectively). Furthermore, the strain showed a good resistance to hydrogen peroxide (76.12%), hydroxyl radicals (46.15%) and a considerable iron chelating ability (20.52 %). Thus, Lb. plantarum 15 could be effectively considered as potent antioxidant probiotic food supplement.

Comparative Studies of Inhibitory and Antioxidant Activities, and Organic Acids Compositions of Postbiotics Produced by Probiotic Lactiplantibacillus plantarum Strains Isolated From Malaysian Foods

“…Since oxidative stress antagonistically influences the occurrence of inflammation, cellular damage, and disease susceptibility in the body (56,57), the postbiotic produced by L. plantarum has the potential to reduce the oxidation of proteins and lipids particularly through two mechanisms, namely, HRS and RP. Unlike other previous studies that reported on the DPPH and ABTS activities of L. plantarum (27,58), two other assays were used in the current research to elucidate further the antioxidant activity of postbiotic produced by L. plantarum. HRS measured the scavenging activity of the most reactive radical, hydroxyl radical, which is related to lipid peroxidation and biomolecules of cells (59).…”

Biological Characterization and Metabolic Variations among Cell-Free Supernatants Produced by Selected Plant-Based Lactic Acid Bacteria

“…Additionally, previous research highlighted the superior free radical scavenging activities of supernatants from various LAB strains ( L. plantarum , L. paracasei , E. faecium, L. helveticus, W. paramesenteroides, and P. pentosaceus ) compared to the control strain L. rhamnosus GG [ 32 ]. Another study conducted demonstrated that the probiotic strain L. plantarum 15 exhibited a DPPH scavenging activity of 75.21% and displayed different levels of reducing power (FRAP) [ 59 ]. Similarly, Han et al [ 60 ] demonstrated that the CFS of L. acidophilus , L. plantarum , L. curvatus, L. sake , P. pentosaceus , and L. fermentum isolated from Harbin dry sausages possessed reducing activities exceeding 1.4 Mm.…”

Therapeutic potential of popular fermented dairy products and its benefits on human health

“…This helps to regulate the immune system and lower serum cholesterol levels. Improves the equilibrium of intestinal microorganisms to promote human health ( 16 ). Certain strains of lactic acid bacteria (LAB) have been observed to elevate the concentrations of pro-inflammatory markers such as TNF-α, IL-1β, and IL-6 while concurrently reducing the expression of anti-inflammatory markers (Arg 1, TGF-β, and CD206).…”