Probiotic properties and immune-stimulating effect of the Jeju lava seawater mineral-coated probiotics

Kim, Kyung Min; Yang, Seung Won; Kim, Du Seong; Lee, Chang-Wan; Kim, Ha Yeon; Lee, Su-Yeon; Choi, Ji-Whi; Sohn, Jungwoo; Shin, Song Seok; Lee, Seunghun

doi:10.1016/j.lwt.2020.109299

Cited by 15 publications

(6 citation statements)

References 31 publications

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“…HDB1258, isolated from breastfeeding infants (data not shown), was identified as Lactobacillus rhamnosus on the basis on the results of Gram staining, 16S rDNA sequencing (GenBank accession number MW193326), and API 50 CHL kit. Lactobacillus rhamnosus HDB1258 (HDB Cell Bank, Hyundae Bioland Co., Ltd., Korea) was inoculated into lava-seawater LAB media containing 8% glucose, 2% yeast extract, 0.5% soy peptone, 0.5% sodium acetate, 0.1% Tween 80, 0.01% MgSO 4 , 0.005% MnSO 4 , 0.2% potassium diphosphate, and 0.2% ammonium sulfate in 30% (v/v) lava-seawater (pH 6.5), incubated at 37°Cfor 20 h, and centrifuged (5000 g, 30 min) [ 36 ]. The resulting precipitate was mixed with hydroxypropyl methylcellulose and trehalose and freeze-dried.…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation

et al. 2021

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Background Gut microbiota closely communicate in the immune system to maintain a balanced immune homeostasis in the gastrointestinal tract of the host. Oral administration of probiotics modulates gut microbiota composition. In the present study, we isolated Lactobacillus rhamnosus HDB1258, which induced tumor necrosis factor (TNF)-α and interleukin (IL)-10 expression in macrophages, from the feces of breastfeeding infants and examined how HDB1258 could regulate the homeostatic immune response in mice with or without lipopolysaccharide (LPS)-induced systemic inflammation. Results Oral administration of HDB1258 significantly increased splenic NK cell cytotoxicity, peritoneal macrophage phagocytosis, splenic and colonic TNF-α expression, TNF-α to IL-10 expression ratio, and fecal IgA level in control mice, while Th1 and Treg cell differentiation was not affected in the spleen. However, HDB1258 treatment significantly suppressed peritoneal macrophage phagocytosis and blood prostaglandin E2 level in mice with LPS-induced systemic inflammation. Its treatment increased LPS-suppressed ratios of Treg to Th1 cell population, Foxp3 to T-bet expression, and IL-10 to TNF-α expression. Oral administration of HDB1258 significantly decreased LPS-induced colon shortening, myeloperoxidase activity and NF-κB+/CD11c+ cell population in the colon, while the ratio of IL-10 to TNF-α expression increased. Moreover, HDB1258 treatment shifted gut microbiota composition in mice with and without LPS-induced systemic inflammation: it increased the Cyanobacteria and PAC000664_g (belonging to Bacteroidetes) populations and reduced Deferribacteres and EU622763_s group (belonging to Bacteroidetes) populations. In particular, PAC001066_g and PAC001072_s populations were negatively correlated with the ratio of IL-10 to TNF-α expression in the colon, while the PAC001070_s group population was positively correlated. Conclusions Oral administered HDB1258 may enhance the immune response by activating innate immunity including to macrophage phagocytosis and NK cell cytotoxicity in the healthy host and suppress systemic inflammation in the host with inflammation by the modulation of gut microbiota and IL-10 to TNF-α expression ratio in immune cells.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation

et al. 2021

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“…Due to higher similarity between oxgall and human bile in bile acid composition, oxgall is often used to evaluate bile tolerance ability of potentially probiotic strains at 0.3%, being close to the mean concentration of human bile acids in the small intestine (Hu et al, 2018b). However, most in vitro bile tolerance tests for potentially probiotic strains published in literatures were carried out under non pH-controlled conditions, which results in a continuous decrease in pH of medium, even below 5.0 depending on the strains and incubation period, during the tests due to carbohydrate fermentation by the strains (Lu et al, 2019;Kim et al, 2020). This led to inability to perform a scientific and reasonable comparison of the strains for their bile tolerance ability, because the strains differ in their facing pH conditions, a very important factor influencing antimicrobial activity of bile acids.…”

Section: Short Communicationmentioning

confidence: 99%

Short communication: Chemical structure, concentration, and pH are key factors influencing antimicrobial activity of conjugated bile acids against lactobacilli

Wang

Chen

Yang

et al. 2021

Journal of Dairy Science

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Effects of chemical structure, concentration, and pH on antimicrobial activity of conjugated bile acids were investigated in 4 strains of lactobacilli. Considerable differences were observed in the antimicrobial activity between the 6 human conjugated bile acids, including glycocholic acid, taurocholic acid, glycodeoxycholic acid, taurodeoxycholic acid, glycochenodeoxycholic acid, and taurochenodeoxycholic acid. Glycodeoxycholic acid and glycochenodeoxycholic acid generally showed significantly higher antimicrobial activity against the lactobacilli, but glycocholic acid and taurocholic acid exhibited the significantly lower antimicrobial activity. Glycochenodeoxycholic acid was selected for further analysis, and the results showed its antimicrobial activity was concentration-dependent, and there was a significantly negative linear correlation (R 2 > 0.98) between bile-antimicrobial index and logarithmic concentration of the bile acid for each strain of lactobacilli. Additionally, the antimicrobial activity of glycochenodeoxycholic acid was also observed to be pH-dependent, and it was significantly enhanced with the decreasing pH, with the result that all the strains of lactobacilli were unable to grow at pH 5.0. In conclusion, chemical structure, concentration, and pH are key factors influencing antimicrobial activity of conjugated bile acids against lactobacilli. This study provides theoretical guidance and technology support for developing a scientific method for evaluating the bile tolerance ability of potentially probiotic strains of lactobacilli.

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“…Also, the viability of probiotics changes their efficacy after consumption. Thus, the amount of input must be at least 10 6 -10 7 CFU/g in order to colonize, to survive in the gut and produce the necessary metabolites (Carvalho et al, 2018;Kim et al, 2020). Daily consumption of fermented foods has been linked to benefits on the immune system and metabolic function (Cassani et al, 2019).…”

Section: Probiotics Of Fermented Foods and Health Effectsmentioning

confidence: 99%

Probiotic Potential of Fermented Foods and Their Effects on Immune System

Şengün

Güney

2021

Turkish JAF Sci.Tech.

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Fermentation is known as a method that improves the nutritional value of foods in addition to preserving them. Various traditionally produced fermented foods such as yogurt, kefir, kombucha, some cheeses, sausages and pickles have probiotic potential. Hence, consumption of fermented foods plays an important role in modulating of gut microbiota and supplying positive effects on health. With the help of beneficial microorganisms, the intestinal barrier can be improved which induces the activity of the immune cells associated to the gut. In recent years, probiotics have been used in foods in order to produce functional foods that have the potential to improve gastrointestinal health. It is known that intestinal microbiota and probiotics can interact in immune system and these interactions provide positive health effects like reducing risk of hypertension, improving gut microbiota, regulating immune system, preventing diabetes, allergies and diarrhea. In this paper, fermented foods having probiotic potential and the effects of consuming these foods on the immune system are revised.

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Probiotic properties and immune-stimulating effect of the Jeju lava seawater mineral-coated probiotics

Cited by 15 publications

References 31 publications

Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation

Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation

Short communication: Chemical structure, concentration, and pH are key factors influencing antimicrobial activity of conjugated bile acids against lactobacilli

Probiotic Potential of Fermented Foods and Their Effects on Immune System

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