Probiotic properties of Debaryomyces hansenii BCS004 and their immunostimulatory effect in supplemented diets for gilthead seabream ( Sparus aurata )

Abstract

Debaryomyces hansenii BCS004 strain is a non‐pathogenic, polyamine and superoxide dismutase secreting marine yeast. The objective of this study was to investigate the marine yeast DhBCS004 as probiotics and evaluate its antimicrobial properties and dietary immunostimulant effect on gilthead seabream specimens when administered at 106 colony‐forming unit/g−1 or 1.1% for 4 weeks. This yeast strain showed potential probiotic properties, including stability in gastrointestinal survival (pH, bile conditions) and attachment to intestinal cells. The DhBCS004 had antimicrobial activity against important marine pathogens. Interestingly, this yeast has a high amount of n−3 polyunsaturated fatty acids with high concentrations of oleic and linoleic acids, which may confer nutritional and therapeutic values. The in vivo immunological assay revealed that dietary DhBCS004 significantly increased phagocytosis capacity, peroxidase and respiratory burst activities in leucocytes. Furthermore, the natural haemolytic complement activity and total IgM levels increased in serum after weeks 2 or 4 of trial. Finally, DhBCS004 significantly increased the transcriptions of TNF‐α, C3 and IgM in intestine samples. This work reinforces the biotechnological and immunological potential of D. hansenii BCS004 in the field of fish aquaculture.

Yeast extract improves growth in rainbow trout (Oncorhynchus mykiss) fed a fishmeal-free diet and modulates the hepatic and distal intestine transcriptomic profile

“…While the liver was more sensitive to the PAP diet, the distal intestine was more sensitive to the PAP+YE diet. These results are consistent with an increase in expression of intestinal complement C3 in response to the use of yeast in gilthead seabream (84,85) . In fish fed the diet PAP+YE, the transcriptomic response of the intestine also revealed the modulation of genes related to mechanisms of pathogen control or allergic reaction.…”

Identification of new yeast strains, Candida zeylanoidesY12‐3 and Hyphopichia pseudoburtoniiY12‐1, from the intestinal tract of rainbow trout, Oncorhynchus mykiss, with potential probiotic characteristics

“…In our study, the identified yeasts were tolerant to bile salts (0.6%–1.5%). Similarly, yeast strains Sporidiobolus ruineniae A45.2, D. hansenii BCS004, Kluyveromyces lactis M3, and Pichia kudriavzevii ONF7.1C have been reported to be tolerant to bile salts (Ghosh et al, 2017; Kanpiengjai et al, 2020; Reyes‐Becerril et al, 2021). Furthermore, the yeast strains jpn01, jpn02, jpn05, and jpn06 isolated from the gut of goldfish grew well and tolerated low pH and bile salt concentrations (Taha et al, 2023).…”

“…In addition, Kanpiengjai et al (2020) reported that Sporidiobolus ruineniae A45.2 was tolerant to pH 2 (Kanpiengjai et al, 2020). It has been noted that the yeast strain D. hansenii BCS004 isolated from surface seawater can also survive under low pH conditions (Reyes-Becerril et al, 2021).…”

“…Recently, four autochthonous yeasts, including Cutaneotrichosporon jirovecii , D. nepalensis , Blastobotrys proliferans , and Diutina catenulata , have also been retrieved from the intestines of goldfish ( Carassius auratus ) with potential probiotic properties (Taha et al, 2023). However, Saccharomyces cerevisiae (Abdel‐Tawwab et al, 2008; Banu et al, 2020; Hoseinifar et al, 2011) and D. hansenii (Angulo et al, 2017; Reyes‐Becerril et al, 2011, 2021; Reyes‐Becerril, Salinas, et al, 2008; Reyes‐Becerril, Tovar‐Ramírez, et al, 2008) have been mostly investigated as probiotics in aquaculture.…”

Perspectives on the Probiotic Potential of Indigenous Moulds and Yeasts in Dry-Fermented Sausages

“…Tolerance to salt, acid pH, and bile salts Antimicrobial activity against pathogenic bacteria in aquaculture (Aeromonas hydrophila, Vibrio vulnificus, Vibrio parahaemolyticus, and Photobacterium damselae) Improvement in the immune response through effect on leucocyte phagocytic capacity, respiratory burst activity, peroxidase activity, serum C3 and IgM levels, and regulation of cytokine gene expression [89] Mouse intestine Mice Increase in the operational taxonomic units of intestinal bacteria Promotion of the growth of beneficial intestinal bacteria [90,91]…”