Probiotic Properties of Pediococcus pentosaceus JBCC 106 and Its Lactic Acid Fermentation on Broccoli Juice

Abstract

To understand the biological roles of Pediococcus pentosaceus strains as probiotics isolated from the traditional Korean fermented food, Jangajji, Pediococcus pentosaceus was selected based on its high cinnamoyl esterase (CE) and antioxidant activities. The acid and bile stability, intestinal adhesion, antagonistic activity against human pathogens, cholesterol-lowering effects, and immune system stimulation without inflammatory effects were evaluated. Nitric oxide (NO) levels were measured in co-culture with various bacterial stimulants. Fermentation ability was measured by using a broccoli matrix and the sulforaphane levels were measured. Resistance to acidic and bilious conditions and 8% adherence to Caco-2 cells were observed. Cholesterol levels were lowered by 51% by assimilation. Moreover, these strains exhibited immunomodulatory properties with induction of macrophage TNF-α and IL-6 and had microstatic effects on various pathogens. Co-culture with various bacterial stimulants resulted in increased NO production. Fermentation activity was increased with the strains, and higher sulforaphane levels were observed. Therefore, in the future, the applicability of the selected strain to broccoli matrix-based fermented functional foods should be confirmed.

Next-Generation Probiotics as Novel Therapeutics for Improving Human Health: Current Trends and Future Perspectives

“…P. pentosaceus has been highlighted for its probiotic effects, including its antibacterial mechanisms, such as the secretion of bacteriocins or bacteriocin-like substances (BLISs) to damage cell walls or to directly kill pathogenic bacteria, effectively combating bacterial threats [ 171 , 172 ]. A study conducted by Sang-Kyu et al showed that P. pentosaceus possesses potential activity against several bacterial species such as S. aureus , S. epidermidis , P. aeruginosa , P. Acnes , P. putida , S. xylosus , E. coli , B. cereus and B. vallismortis [ 173 ]. The use of specific strains of beneficial bacteria, such as Lactobacillus , Bifidobacterium , and B. coagulans , has been shown to target specific pathogens and modulate the immune system, enhancing the body’s natural defenses against infections.…”