Probiotic Strains Influence on Infant Microbiota in the In Vitro Colonic Fermentation Model GIS1

Moroeanu, Veronica; Vamanu, Emanuel; Păun, Gabriela; Neagu, Elena; Ungureanu, Oana; Eremia, Sandra A. V.; Radu, Gabriel Lucian; Ionescu, Robertina; Pelinescu, Diana

doi:10.1007/s12088-015-0542-8

Cited by 13 publications

(9 citation statements)

References 23 publications

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“…In this regard, probiotic and prebiotic dietary fibres (DF) are thought to be useful in mitigating the inflammatory circuit thereby resolving or preventing the severity of IBD. Both bioactive ingredients can improve inflammatory parameters in the gut by modifying microbiota composition and metabolites, regulating secretion of immunomodulatory molecules and protecting the colonic epithelial barrier [1,6,7,8]. Synbiotics, being a combination of probiotic and prebiotic ingredients that positively interact, potentially offer prophylactic and therapeutic effects that could function synergistically to confer health benefits to the host.…”

Section: Introductionmentioning

confidence: 99%

Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

et al. 2019

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Inflammatory bowel diseases (IBD) are a chronic inflammatory disorders with increasing global incidence. Synbiotic, which is a two-point approach carrying probiotic and prebiotic components in mitigating inflammation in IBD, is thought to be a pragmatic approach owing to the synergistic outcomes. In this study, the impacts of dietary supplementation with probiotic Bacillus coagulans MTCC5856 spores (B. coagulans) and prebiotic whole plant sugar cane fibre (PSCF) was assessed using a murine model of IBD. Eight-week-old C57BL/6 mice were fed a normal chow diet supplemented with either B. coagulans, PSCF or its synbiotic combination. After seven days of supplementation, colitis was induced with dextran sulfate sodium (DSS) in drinking water for seven days during the continuation of the supplemented diets. Synbiotic supplementation ameliorated disease activity index and histological score (−72%, 7.38, respectively), more effectively than either B. coagulans (−47%, 10.1) and PSCF (−53%, 13.0) alone. Synbiotic supplementation also significantly (p < 0.0001) prevented the expression of tight junction proteins and modulated the altered serum IL-1β (−40%), IL-10 (+26%), and C-reactive protein (CRP) (−39%) levels. Synbiotic supplementations also raised the short-chain fatty acids (SCFA) profile more extensively compared to the unsupplemented DSS-control. The synbiotic health outcome effect of the probiotic and prebiotic combinations may be associated with a synergistic direct immune-regulating efficacy of the components, their ability to protect epithelial integrity, stimulation of probiotic spores by the prebiotic fibre, and/or with stimulation of greater levels of fermentation of fibres releasing SCFAs that mediate the reduction in colonic inflammation. Our model findings suggest synbiotic supplementation should be tested in clinical trials.

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Section: Introductionmentioning

confidence: 99%

Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

et al. 2019

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“…In vitro studies reduce issues with ethical restrictions and volunteer compliance, while enabling more frequent sampling and providing a simplified system to study the gut microbiota without host interference 21 . In vitro infant gut microbiota model systems have been employed to investigate the effect of probiotics 22 , candidate probiotics 23 , milk lipid hydrolysis products 24 , iron 25 , milk oligosaccharides 26 – 28 , dietary polysaccharides and prebiotics 29 – 31 . Addition of short-chain fructo-oligosaccharides into an in vitro model of infant gut microbiota resulted in an increased abundance of the genus Lactobacillus while reducing the proportion of coliforms 29 .…”

Section: Introductionmentioning

confidence: 99%

Cereal products derived from wheat, sorghum, rice and oats alter the infant gut microbiota in vitro

Gamage

Tetu

Chong

et al. 2017

Sci Rep

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The introduction of different nutrient and energy sources during weaning leads to significant changes in the infant gut microbiota. We used an in vitro infant digestive and gut microbiota model system to investigate the effect of four commercially available cereal products based on either wheat, sorghum, rice or oats, on the gut microbiota of six infants. Our results indicated cereal additions induced numerous changes in the gut microbiota composition. The relative abundance of bacterial families associated with fibre degradation, Bacteroidaceae, Bifidobacteriaceae, Lactobacillaceae, Prevotellaceae, Ruminococcaceae and Veillonellaceae increased, whilst the abundance of Enterobacteriaceae decreased with cereal additions. Corresponding changes in the production of SCFAs showed higher concentrations of acetate following all cereal additions, whilst, propionate and butyrate varied between specific cereal additions. These cereal-specific variations in the concentrations of SCFAs showed a moderate correlation with the relative abundance of potential SCFA-producing bacterial families. Overall, our results demonstrated clear shifts in the abundance of bacterial groups associated with weaning and an increase in the production of SCFAs following cereal additions.

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“…Microencapsulation has a significant effect in this case. Microencapsulation technologies have been extended and applied effectively to protect the probiotic bacteria from damages caused via the exterior milieu in the situations such as processing (high temperature), storage (in the food products on the shelf and in a home like foodstuff matrix), packaging (temperature, oxygen, humidity) and degradation in the GI region (the low pH in the stomach and bile salt in the small intestine) (Anal and Singh 2007 ; Moroeanu et al 2015 ; Zuidam and Nedovic 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of Eudragit S100 nanoparticles and alginate chitosan encapsulation on the viability of Lactobacillus acidophilus and Lactobacillus rhamnosus

et al. 2017

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In this study, we examined a novel method of microencapsulation with calcium alginate-chitosan and Eudragit S100 nanoparticles for the improving viability of probiotic bacteria, Lactobacillus acidophilus and Lactobacillus rhamnosus. Extrusion technique was carried out in microencapsulation process. The viability of two probiotics in single coated beads (with only chitosan), double coated beads (with chitosan and Eudragit nanoparticles), and as free cells (unencapsulated) were conducted in simulated gastric juice (pH 1.55, without pepsin) followed by incubation in simulated intestinal juice (pH 7.5, with 1% bile salt). In case of single coated beads, presumably, lack of sufficient strength of chitosan under simulated gastric condition was the main reason of 4-log and 5-log reduction of the counts of the L. acidophilus and L. rhamnosus respectively. The results showed that with the second coat forming (Eudragit nanoparticles) over the first coat (chitosan), the strength of the beads and then viability rate of the bacteria were increased in comparison with the single coated beads.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0442-x) contains supplementary material, which is available to authorized users.

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Probiotic Strains Influence on Infant Microbiota in the In Vitro Colonic Fermentation Model GIS1

Cited by 13 publications

References 23 publications

Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD

Cereal products derived from wheat, sorghum, rice and oats alter the infant gut microbiota in vitro

Effect of Eudragit S100 nanoparticles and alginate chitosan encapsulation on the viability of Lactobacillus acidophilus and Lactobacillus rhamnosus

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