Probiotic Treatment of Small Intestinal Bacterial Overgrowth by Lactobacillus fermentum KLD

Stotzer, Per-Ove; Blomberg, Lena; Conway, Patricia L.; Henriksson, A.; Abrahamsson, Hasse

doi:10.3109/00365549609037970

Cited by 43 publications

(19 citation statements)

References 17 publications

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“…A comprehensive understanding of microbial interactions that occur in the intestinal tract in addition to alterations that occur upon exposure to high levels of an exogenous strain is essential for scienti¢c acceptance of the probiotic rationale. Although it has been suggested that L. fermentum KLD has good potential as a probiotic strain [36], other workers reported that the strain exhibited poor pharmacokinetic properties [37] and displayed little probiotic promise when used in treatment of small intestine bacterial overgrowth [26]. This study provides evidence that the introduction of KLD to the murine gastrointestinal tract induces shifts in indigenous microbial communities.…”

Section: Discussionmentioning

confidence: 66%

“…Lactobacillus fermentum KLD has previously been used as both a prophylactic and therapeutic agent in the treatment of gastrointestinal disturbances [25,26] and is an interesting candidate probiotic strain. Using the murine system as model for the complex bacterial community of the gastrointestinal tract, we sought to assess the impact of administration of this strain and to examine the biodiversity within faecal samples from L. fermentum-treated mice using conventional techniques, PCR and DGGE.…”

Section: Introductionmentioning

confidence: 99%

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Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Plant

2003

FEMS Microbiology Ecology

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The indigenous gastrointestinal microbiota acts as an integral defense against the colonisation of orally introduced microbes. Whilst this can be important in host protection, some introduced species, including lactobacilli, can have a positive impact on existing microbial communities. The interaction of a candidate probiotic strain of Lactobacillus fermentum within the gastrointestinal tract was monitored in a mouse model and its effect on the indigenous microbiota observed. L. fermentum KLD was administered via oro-gastric doses to mice with both a specific pathogen-free (SPF) and an ampicillin-depleted gut microbiota, containing no detectable lactobacilli. Its persistence was monitored by detection in faecal homogenates using culturing methods and polymerase chain reaction with L. fermentum specific primers. Microbial population shifts were observed using denaturing gradient gel electrophoresis (DGGE). L. fermentum KLD was detected within the gastrointestinal tract of SPF mice for up to 36 h, and for greater than 11 days in the ampicillin-treated mice. The administration resulted in substantial changes within the host Lactobacillus levels, determined by DGGE of 16S rDNA from faecal samples. Denaturing gradient profiles, from faecal samples collected at a range of pre-and post-dose intervals of groups of 10 SPF mice, indicated that several other constituents of the gastrointestinal community also fluctuated following dosing. These included Bifidobacterium and Eubacterium, which increased following KLD administration. The indigenous microbiota affected the persistence of L. fermentum KLD and in SPF mice the administration of this strain induced significant shifts in the indigenous microbial community. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Section: Discussionmentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Plant

2003

FEMS Microbiology Ecology

View full text Add to dashboard Cite

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“…A comprehensive understanding of microbial interactions that occur in the intestinal tract in addition to alterations that occur upon exposure to high levels of an exogenous strain is essential for scientific acceptance of the probiotic rationale. Although it has been suggested that L. fermentum KLD has good potential as a probiotic strain [36], other workers reported that the strain exhibited poor pharmacokinetic properties [37] and displayed little probiotic promise when used in treatment of small intestine bacterial overgrowth [26]. This study provides evidence that the introduction of KLD to the murine gastrointestinal tract induces shifts in indigenous microbial communities.…”

Section: Discussionmentioning

confidence: 69%

“…Lactobacillus fermentum KLD has previously been used as both a prophylactic and therapeutic agent in the treatment of gastrointestinal disturbances [25,26] and is an interesting candidate probiotic strain. Using the murine system as model for the complex bacterial community of the gastrointestinal tract, we sought to assess the impact of administration of this strain and to examine the biodiversity within faecal samples from L. fermentum ‐treated mice using conventional techniques, PCR and DGGE.…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Plant

Lam

Conway

et al. 2003

FEMS Microbiology Ecology

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The indigenous gastrointestinal microbiota acts as an integral defense against the colonisation of orally introduced microbes. Whilst this can be important in host protection, some introduced species, including lactobacilli, can have a positive impact on existing microbial communities. The interaction of a candidate probiotic strain of Lactobacillus fermentum within the gastrointestinal tract was monitored in a mouse model and its effect on the indigenous microbiota observed. L. fermentum KLD was administered via oro‐gastric doses to mice with both a specific pathogen‐free (SPF) and an ampicillin‐depleted gut microbiota, containing no detectable lactobacilli. Its persistence was monitored by detection in faecal homogenates using culturing methods and polymerase chain reaction with L. fermentum specific primers. Microbial population shifts were observed using denaturing gradient gel electrophoresis (DGGE). L. fermentum KLD was detected within the gastrointestinal tract of SPF mice for up to 36 h, and for greater than 11 days in the ampicillin‐treated mice. The administration resulted in substantial changes within the host Lactobacillus levels, determined by DGGE of 16S rDNA from faecal samples. Denaturing gradient profiles, from faecal samples collected at a range of pre‐ and post‐dose intervals of groups of 10 SPF mice, indicated that several other constituents of the gastrointestinal community also fluctuated following dosing. These included Bifidobacterium and Eubacterium, which increased following KLD administration. The indigenous microbiota affected the persistence of L. fermentum KLD and in SPF mice the administration of this strain induced significant shifts in the indigenous microbial community.

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“…However, no protection against travelers’ diarrhea could be demonstrated [39]. Also, there was no effect on the hydrogen breath test, symptom score and number of defecations in patients with bacterial overgrowth [54].…”

Section: Randomized Double‐blind Studies With Pharmaceutical Bacteriamentioning

confidence: 99%

Bacteria and yeasts in the treatment of acute and chronic infectious diarrhea. Part I. Bacteria

Vandenplas¹

1999

Clinical Microbiology and Infection

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Treatment of acute infectious gastroenteritis consists mainly of rehydration and rapid realimentation. However, the natural intestinal microflora is disrupted in both acute and chronic infectious diarrhea, resulting in complex interactions possibly aggravating this frequently self-limiting condition. Therefore, additional therapeutic intervention with biotherapeutic agents is worth considering. The results of most randomized prospective double-blind clinical trials with bacterial biotherapeutic agents are disappointing, showing a lack of efficacy, except for Lactobacillus casei strain GG, for which both positive and negative results have been published.

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Probiotic Treatment of Small Intestinal Bacterial Overgrowth by Lactobacillus fermentum KLD

Cited by 43 publications

References 17 publications

Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Gastrointestinal microbial community shifts observed following oral administration of a Lactobacillus fermentum strain to mice

Bacteria and yeasts in the treatment of acute and chronic infectious diarrhea. Part I. Bacteria

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