Probiotics and Intestinal Colonization by Vancomycin-Resistant Enterococci in Mice and Humans

Vidal, M.; Forestier, Christiane; Charbonnel, Nicolas; Hénard, S.; Lesens, Olivier

doi:10.1128/jcm.00473-10

Cited by 35 publications

(34 citation statements)

References 9 publications

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“…Their results showed the probiotic's efficacy in clearing VRE from fecal specimens (22). However, more recently, Lactobacillus rhamnosus Lcr35 has been shown to have little or no effect in eradicating or preventing VRE intestinal colonization in mice or human patients (23). The effect of a probiotic product containing different species (Bifidobacterium bifidum, B. lactis, Enterococcus faecium, Lactobacillus acidophilus L. paracasei, L. plantarum, L. rhamnosus, and L. salivarius) against ampicillin-resistant enterococcal intestinal colonization was studied in wards with a documented high prevalence of such bacteria, but the probiotics could not prevent colonization by the resistant enterococci (24).…”

Section: Discussionmentioning

confidence: 99%

“…During the week, daily clinical examinations consisted of evaluations for general clinical signs and recording of rectal temperatures. Individual fecal samples were collected from all animals on day 0 before inoculation and on days 1, 3, 7, 10, 13, 17, 22, and 28 (the first trial) and days 3,8,10,13,16,20,23,27, and 31 (the second trial). The fecal samples were stored at Ϫ70°C until analysis.…”

Section: Methodsmentioning

confidence: 99%

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Escherichia coli Probiotic Strain ED1a in Pigs Has a Limited Impact on the Gut Carriage of Extended-Spectrum-β-Lactamase-Producing E. coli

Mourand

Paboeuf

Fleury

et al. 2017

Antimicrob Agents Chemother

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Four trials were conducted to evaluate the impact of Escherichia coli probiotic strain ED1a administration to pigs on the gut carriage or survival in manure of extended-spectrum-␤-lactamase-producing E. coli. Groups of pigs were orally inoculated with strain E. coli M63 carrying the bla CTX-M-1 gene (n ϭ 84) or used as a control (n ϭ 26). In the first two trials, 24 of 40 E. coli M63-inoculated pigs were given E. coli ED1a orally for 6 days starting 8 days after oral inoculation. In the third trial, 10 E. coli M63-inoculated pigs were given either E. coli ED1a or probiotic E. coli Nissle 1917 for 5 days. In the fourth trial, E. coli ED1a was given to a sow and its 12 piglets, and these 12 piglets plus 12 piglets that had not received E. coli ED1a were then inoculated with E. coli M63. Fecal shedding of cefotaxime-resistant Enterobacteriaceae (CTX-RE) was studied by culture, and bla CTX-M-1 genes were quantified by PCR. The persistence of CTX-RE in manure samples from inoculated pigs or manure samples inoculated in vitro with E. coli M63 with or without probiotics was studied. The results showed that E. coli M63 and ED1a were good gut colonizers. The reduction in the level of fecal excretion of CTX-RE in E. coli ED1a-treated pigs compared to that in nontreated pigs was usually less than 1 log 10 CFU and was mainly observed during the probiotic administration period. The results obtained with E. coli Nissle 1917 did not differ significantly from those obtained with E. coli ED1a. CTX-RE survival did not differ significantly in manure samples with or without probiotic treatment. In conclusion, under our experimental conditions, E. coli ED1a and E. coli Nissle 1917 could not durably prevent CTX-RE colonization of the pig gut.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Escherichia coli Probiotic Strain ED1a in Pigs Has a Limited Impact on the Gut Carriage of Extended-Spectrum-β-Lactamase-Producing E. coli

Mourand

Paboeuf

Fleury

et al. 2017

Antimicrob Agents Chemother

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“…These bacteria are currently used in probiotic dietary products, and several lines of evidence have demonstrated their beneficial effects on gut homeostasis (6,16,20,38,44). In addition, mouse studies have been conducted to investigate the immunomodulatory capacity of these potential probiotics, with similar findings (18,23,43,46). These effects range from downregulation of cytokine responses in immune cells to induction of apoptosis in T cells and vaccine-improving properties (4,7,8,10,13).…”

mentioning

confidence: 89%

Differential Toll-Like Receptor Recognition and Induction of Cytokine Profile by Bifidobacterium breve and Lactobacillus Strains of Probiotics

Plantinga¹,

Maren²,

Bergenhenegouwen³

et al. 2011

Clin. Vaccine Immunol.

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The use of probiotics as a food supplement has gained tremendous interest in the last few years as beneficial effects were reported in gut homeostasis and nutrient absorption but also in immunocompromised patients, supporting protection from colonization or infection with pathogenic bacteria or fungi. As a treatment approach for inflammatory bowel diseases, a suitable probiotic strain would ideally be one with a low immunogenic potential. Insight into the immunogenicities and types of T-cell responses induced by potentially probiotic strains allows a more rational selection of a particular strain. In the present study, the bacterial strains Bifidobacterium breve (NumRes 204), Lactobacillus rhamnosus (NumRes1), and Lactobacillus casei (DN-114 001) were compared concerning their capacity to induce inflammatory responses in terms of cytokine production by human and mouse primary immune cells. It was demonstrated that the B. breve strain induced lower levels of the proinflammatory cytokine gamma interferon (IFN-␥) than the tested L. rhamnosus and L. casei strains. Both B. breve and lactobacilli induced cytokines in a Toll-like receptor 9 (TLR9)-dependent manner, while the lower inflammatory profile of B. breve was due to inhibitory effects of TLR2. No role for TLR4, NOD2, and C-type lectin receptors was apparent. In conclusion, TLR signaling is involved in the differentiation of inflammatory responses between probiotic strains used as food supplements.

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“…26 Similarly, we found that the use of probiotics did not prevent development of resistant microorganisms in preterm infants. The limitations of our study were the small sample size and the lack of ability to culture anaerobic microorganisms.…”

Section: Discussionmentioning

confidence: 72%

Effect of oral administration of probiotics on intestinal colonization with drug-resistant bacteria in preterm infants

Kurt

Ince

Ecevit

et al. 2017

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Probiotics and Intestinal Colonization by Vancomycin-Resistant Enterococci in Mice and Humans

Cited by 35 publications

References 9 publications

Escherichia coli Probiotic Strain ED1a in Pigs Has a Limited Impact on the Gut Carriage of Extended-Spectrum-β-Lactamase-Producing E. coli

Escherichia coli Probiotic Strain ED1a in Pigs Has a Limited Impact on the Gut Carriage of Extended-Spectrum-β-Lactamase-Producing E. coli

Differential Toll-Like Receptor Recognition and Induction of Cytokine Profile by Bifidobacterium breve and Lactobacillus Strains of Probiotics

Effect of oral administration of probiotics on intestinal colonization with drug-resistant bacteria in preterm infants

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