Probiotics (Lactobacillus plantarum HNU082) Supplementation Relieves Ulcerative Colitis by Affecting Intestinal Barrier Functions, Immunity-Related Gene Expression, Gut Microbiota, and Metabolic Pathways in Mice

Wu, Yuqing; Jha, Rajesh; Li, Ao; Liu, Huanwei; Zeng, Zhen; Zhang, Chengcheng; Zhai, Qixiao; Zhang, Jiachao

doi:10.1128/spectrum.01651-22

Cited by 92 publications

(45 citation statements)

References 86 publications

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“…40 HumanN2 metagenomic metabolic pathway annotations were constructed based on the UniRef90 database. 41…”

Section: Methodsmentioning

confidence: 99%

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Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Chen

et al. 2022

Food Funct.

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“…40 HumanN2 metagenomic metabolic pathway annotations were constructed based on the UniRef90 database. 41…”

Section: Methodsmentioning

confidence: 99%

“…40 HumanN2 metagenomic metabolic pathway annotations were constructed based on the UniRef90 database. 41 The R software and GraphPad software were used for statistical analysis. The Wilcoxon rank-sum test was performed, and P values < 0.05 were considered to be significant.…”

Section: Rna Extraction Pcr Amplification Electrophoresis and 16s Rrn...mentioning

confidence: 99%

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Chen

et al. 2022

Food Funct.

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“…in preliminary experiments as a supplement for tacrolimus treatment. In recent years, some strains from Lactobacillus plantarum were reported to attenuate colitis in mice by restoring the disturbed gut microbiota, affecting intestinal barrier functions and immunity-related gene expression( Sun et al., 2020 ; Wu et al., 2022 ). Our results showed better remission of colitis in the combination treatment group than in the tacrolimus monotherapy group, accompanied by further downregulation of inflammatory signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Combination of Lactobacillus plantarum improves the effects of tacrolimus on colitis in a mouse model

Zhang

et al. 2023

Front. Cell. Infect. Microbiol.

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The gut microbiome has been considered to play an important role in inflammatory bowel disease (IBD). Our previous study reported that tacrolimus-altered gut microbiota elicited immunoregulatory effects in both colonic mucosa and circulation, contributing to an increased allograft survival rate in mice. Here, we aimed to observe the changes in the tacrolimus-induced microbiome in a dextran sulfate sodium (DSS)-induced colitis mouse model and explore the possibility and efficacy of combination therapy with tacrolimus and the microbiome on colitis. Mice were divided into the control, DSS, tacrolimus monotherapy and tacrolimus plus Lactobacillus plantarum 550 (Lacto)-treated groups. The body weight, stool consistency, hematochezia and survival of mice were observed daily. Total RNA from colonic mucosa was extracted and subjected to transcriptome sequencing. Cecal contents were collected and the 16S rRNA sequencing was performed to characterize the gut microbiome and the ultrahigh- performance liquid chromatography-MS/MS (UHPLC-MS/MS) was used for targeted quantification of bile acids. The results confirmed that tacrolimus significantly ameliorated DSS-induced colitis in mice. Beneficial alterations of the gut microbiome characterized by a remarkable expansion of the genus Lactobacillus were induced by tacrolimus treatment. Oral supplementation with Lacto further improved the tacrolimus-mediated suppression of body weight loss in colitis, while the survival time of mice was further prolonged and the inflammation of colonic mucosa was obviously relieved. The immune and inflammation-related signaling pathways, including IFN-γ and IFN-α response, allograft rejection, IL2 STAT5 signaling and the inflammatory response pathways, were further downregulated in the tacrolimus plus Lacto cotreatment group. Cotreatment also improved the diversity of the gut microbiome and rescued the concentration of taurochenodeoxycholic acid (TCDCA) in colitis. The latter was positively correlated with the abundance of Lactobacillus but negatively related to the disease activity index score. Overall, our results indicated that Lactobacillus plantarum promoted the therapeutic effect of tacrolimus in experimental colitis, offering a promising strategy to combine tacrolimus and Lactobacillus in the treatment of colitis patients.

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“…Impaired intestinal barrier function leads to intestinal microbial translocation, promotes the production of pro-inflammatory cytokines, and further promotes the high activation of mucosal immune system, thus leading to inflammation in IBD patients ( 38 , 39 ). Previous works have shown the anti-inflammatory effect of ginsenoside Rg1 in mice with DSS-induced colitis ( 7 ).…”

Section: Discussionmentioning

confidence: 99%

Ameliorative effect of ginsenoside Rg1 on dextran sulfate sodium-induced colitis: involvement of intestinal barrier remodeling in mice

He¹,

Liang²,

Sun³

2022

Ann Transl Med

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Background: Ginsenoside Rg1, a major bioactive ingredient of Panax notoginseng, has been shown to reduce gut inflammation and ameliorate experimental colitis in mice. However, it is not yet known whether it affects the intestinal barrier injury of colitis.Methods: This study explored the effect of ginsenoside Rg1 on intestinal barrier injury in dextran sulfate sodium (DSS)-induced colitis mice through an ultrastructure observation of the colonic mucosa and analysis of the expression of colonic cytoplasmatic zonula occludens-1 (ZO-1) protein.Results: Treatment with ginsenoside Rg1, especially high-dose use, significantly ameliorated colonic histopathologic features and the severity of the colitis and reduced colonic tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) levels and increase IL-4 levels in a mouse model of DSS-induced colitis. Its observed efficacy was comparable to that of 5-Aminosalicylic acid (5-ASA), a first-line therapeutic agent for ulcerative colitis. Notably, ginsenoside Rg1 administration was shown to up-regulate the expression of colonic ZO-1 protein, and it repaired the intestinal barrier structure in DSS-induced colitis mice.Conclusions: Taken together, our findings demonstrated that ginsenoside Rg1 treatment can significantly ameliorate the severity of DSS-induced colitis in mice, which involves intestinal barrier structure remodeling through lowering the levels of the colonic pro-inflammatory cytokines TNF-α and IFN-γ and increasing the anti-inflammatory cytokine IL-4. These results suggest the potential therapeutic use of ginsenoside Rg1 as a promising approach for the treatment of inflammatory bowel disease (IBD).

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Probiotics (Lactobacillus plantarum HNU082) Supplementation Relieves Ulcerative Colitis by Affecting Intestinal Barrier Functions, Immunity-Related Gene Expression, Gut Microbiota, and Metabolic Pathways in Mice

Abstract: Many studies have focused on the therapeutic effect of probiotics on ulcerative colitis (UC), but few studies have paid attention to the mechanism of probiotics, especially the therapeutic effect. This study suggests that Lp082 has a therapeutic effect on colitis in mice.

Cited by 92 publications

References 86 publications

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Deep Shotgun metagenomic and 16S rRNA analysis revealed the microbial diversity of lactic acid bacteria in traditional fermented foods of eastern Hainan, China

Combination of Lactobacillus plantarum improves the effects of tacrolimus on colitis in a mouse model

Ameliorative effect of ginsenoside Rg1 on dextran sulfate sodium-induced colitis: involvement of intestinal barrier remodeling in mice

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