Probiotics lower plasma glucose in the high-fat fed C57BL/6J mouse

Abstract

Today, the gut microbiota is considered a key organ in host nutritional metabolism and recent data have suggested that alterations in gut microbiota contribute to the development of type 2 diabetes and obesity. Accordingly, a whole range of beneficial effects relating to inflammation and gut health have been observed following administration of probiotics to both humans and different animal models. The objective of this study was to evaluate the metabolic effects of an oral probiotic supplement, Lactobacillus plantarum DSM 15313, to high-fat diet (HFD) fed C57BL/6J mice, a model of human obesity and early diabetes. The mice were fed the experimental diets for 20 weeks, after which the HFD had induced an insulin-resistant state in both groups compared to the start of the study. The increase in body weight during the HFD feeding was higher in the probiotic group than in the control group, however, there were no significant differences in body fat content. Fasting plasma glucose levels were lower in the group fed the probiotic supplement, whereas insulin and lipids were not different. Caecal levels of short-chain fatty acids were not significantly different between the groups. An oral glucose tolerance test showed that the group fed probiotics had a significantly lower insulin release compared to the control group, although the rate of glucose clearance was not different. Taken together, these data indicate that L. plantarum DSM 15313 has anti-diabetic properties when fed together with an HFD.

The proteins (12 and 15 kDa) isolated from heat‐killed Lactobacillus plantarum L67 induces apoptosis in HT‐29 cells

“…[6][7][8][9] In particular, Lactobacillus plantarum exhibits various biological effects including antitumour, anticoagulant, antiviral, immune modulatory and anti-inflammatory, antidiabetic and antioxidant or free radical scavenging activity. [10][11][12][13] We reported that L. plantarum L67 isolated from infant faeces has a high survival rate at low pH values. The aim of this study was to determine whether a protein isolated from L. plantarum L67 induces programmed cell death in HT-29 cells.…”

Incorporation of therapeutically modified bacteria into gut microbiota inhibits obesity

“…In addition to our genetically modified EcN, a number of unmodified probiotic bacteria also show antiobesity effects in mice (69)(70)(71)(72)(73). One clear advantage of using genetically modified gut microbiota rather than wild-type probiotics is the ability to choose both an appropriate carrier bacteria that can colonize the gut of the diseased individual and an appropriate therapeutic compound, whereas most probiotic bacteria are poor colonizers and the actual bioactive metabolites that confer their benefit poorly characterized.…”

Lactobacillus Paracasei NFRI 7415 Reduces Liver Lipid Contents in C57BL/6J Mice Fed a High-fat Diet

“…Andersson et al [10] reported the protective effects of a strain of Lactobacillus plantarum on obesity and early diabetes in, to highfat diet (HFD) fed C57BL/6J mice fed a high-fat diet for 20 weeks. Despite L. plantarum decreasing the plasma glucose level in a highfat diet fed mice, the energy intake, body fat content and plasma cholesterol were not different compared to the control group.…”

“…For example, Lactobacillus plantarum and Lactobacillus gasseri may exert a beneficial effect on the onset of diet-induced obesity by reducing the cell size of white adipose tissues [8,9]. Andersson et al [10] indicate that Lactobacillus plantarum DSM 15313 has anti-diabetic properties in the context of a high-fat diet.…”