Probiyotiklerin Kültürde Mezenkimal Kök Hücre Davranışına Etkisi

Abstract

Aim: Probiotic (PB) and Mesenchymal Stem Cell (MSC) are new treatment ideas for diseases. Alterations of microbiata made by PB produce different effects. MSC as the scope of cellular therapy takes place in clinical practice in many diseases such as Graft Versus Host (GVD) within the scope of cellular therapy. In this study, the effects of PB application on MSC behavior in culture in terms of proliferation, migration, differentiation and wound healing were investigated. Material and Method: Frozen adipose tissue-derived (AD) MSC cells were cultured and produced at 2.5x104 cells/cm2 in a twelve-well culture dish. They were examined for proliferation by MTT, migration by the closure of the wound after the creation of a scratch, and differentiation by transforming the bone cell osteoblast. NBL Probiotic Gold as PB was used as bacteria which include 2.5x109 Enterococcus faecium, 2.5x109lactobacillus acidophilus, 2.5x109lactobacillus rhamnosus, 2.5x109Bifidobacterium longum, 2.5x109Bifidobacterium bifidum and examined in terms of behavioral parameters for MSC in the same environment. The relationship between PB and MSC was also investigated by Scanning Electron Microscopy (SEM). Result: It was determined that use of PB caused an increase in proliferation, faster wound closure by increase of migration and higher differentiation to osteoblast. The ultrastructural examination of MSC by SEM showed that MSCs interacted with PB are more spread, more adherent, and have more active cell morphology. Conclusion:The results showed that the addition of PB to the culture medium increased the MSC efficiency. It was thought that PB would be more beneficial for the clinic to see of MSC if the same positive effect results in studies with experimental animals.