Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories

Tang, Ruohao; Yuan, Xian-Zheng; Jian-ming, Yang

doi:10.1016/j.biotechadv.2023.108183

Cited by 11 publications

(1 citation statement)

References 131 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The excess NADPH, functioning as a reductant, might induce oxidative stress in cells [ 31 ] and further manifest as growth delay or lower production property. Based on KEGG annotations [ 32 ], the cbb c operon in the chromosome and the cbb p operon in megaplasmid pHG1 were expressed independently, both capable of achieving CO 2 fixation using NADPH as a coenzyme [ 33 , 34 ]. The balanced NADPH may explain why the extended lag phase caused by gnd gene expression only occurs in heterotrophic fermentation.…”

Section: Discussionmentioning

confidence: 99%

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

Wang,

Luo,

Yao

et al. 2023

IJMS

View full text Add to dashboard Cite

Cupriavidus necator is a versatile microbial chassis to produce high-value products. Blocking the poly-β-hydroxybutyrate synthesis pathway (encoded by the phaC1AB1 operon) can effectively enhance the production of C. necator, but usually decreases cell density in the stationary phase. To address this problem, we modified the hexose utilization pathways of C. necator in this study by implementing strategies such as blocking the Entner–Doudoroff pathway, completing the phosphopentose pathway by expressing the gnd gene (encoding 6-phosphogluconate dehydrogenase), and completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene (encoding 6-phosphofructokinase). During heterotrophic fermentation, the OD600 of the phaC1AB1-knockout strain increased by 44.8% with pfkA gene expression alone, and by 93.1% with gnd and pfkA genes expressing simultaneously. During autotrophic fermentation, gnd and pfkA genes raised the OD600 of phaC1AB1-knockout strains by 19.4% and 12.0%, respectively. To explore the effect of the pfkA gene on the production of C. necator, an alanine-producing C. necator was constructed by expressing the NADPH-dependent L-alanine dehydrogenase, alanine exporter, and knocking out the phaC1AB1 operon. The alanine-producing strain had maximum alanine titer and yield of 784 mg/L and 11.0%, respectively. And these values were significantly improved to 998 mg/L and 13.4% by expressing the pfkA gene. The results indicate that completing the Embden–Meyerhof–Parnas pathway by expressing the pfkA gene is an effective method to improve the growth and production of C. necator.

show abstract

Section: Discussionmentioning

confidence: 99%

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

Wang,

Luo,

Yao

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing

Federici,

Orsi,

Nikel

2023

ChemCatChem

View full text Add to dashboard Cite

Industrial chemical production largely relies on fossil fuels, resulting in the unavoidable release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon bioeconomy has been proposed to address this issue, wherein CO2 is captured and used as raw material for manufacturing new chemicals. Microbial cell factories and, in particular, autotrophic microorganisms capable of utilizing CO2 as the sole carbon source, emerged as potential catalysts for upcycling CO2 to valuable products. The Calvin‐Benson‐Bassham cycle (CBBc), the best‐known CO2 fixation pathway, is widely distributed in Nature. While extensively studied, microbial engineering programmes based on the CBBc remains relatively underexplored. In this review, we discuss avenues towards biotechnological exploitation of the CBBc to engineer CO2‐utilizing microbial cell factories, with a focus on chemically‐derived electron donors. We also highlight the advantages and challenges of implementing the CBBc in heterotrophic microbial hosts and its potential to advance a true circular carbon bioeconomy. Moreover, based on the pathway’s architecture, we argue about the ideal value‐added products to generate from this metabolic route. Studying and engineering the CBBc in both natural‐ and synthetic‐autotrophs will enhance our understanding on this CO2 fixation pathway, enabling further exploration of biomanufacturing avenues with CO2 as feedstock.

show abstract

Advances in Defect Engineering of Metal Oxides for Photocatalytic CO₂ Reduction

Zhong,

Sun,

2024

Small

View full text Add to dashboard Cite

Photocatalytic CO2 reduction technology, capable of converting low‐density solar energy into high‐density chemical energy, stands as a promising approach to alleviate the energy crisis and achieve carbon neutrality. Semiconductor metal oxides, characterized by their abundant reserves, good stability, and easily tunable structures, have found extensive applications in the field of photocatalysis. However, the wide bandgap inherent in metal oxides contributes to their poor efficiency in photocatalytic CO2 reduction. Defect engineering presents an effective strategy to address these challenges. This paper reviews the research progress in defect engineering to enhance the photocatalytic CO2 reduction performance of metal oxides, summarizing defect classifications, preparation methods, and characterization techniques. The focus is on defect engineering, represented by vacancies and doping, for improving the performance of metal oxide photocatalysts. This includes advancements in expanding the photoresponse range, enhancing photogenerated charge separation, and promoting CO2 molecule activation. Finally, the paper provides a summary of the current issues and challenges faced by defect engineering, along with a prospective outlook on the future development of photocatalytic CO2 reduction technology.

show abstract

Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories

Cited by 11 publications

References 131 publications

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing

Advances in Defect Engineering of Metal Oxides for Photocatalytic CO₂ Reduction

Contact Info

Product

Resources

About

Problems and corresponding strategies for converting CO2 into value-added products in Cupriavidus necator H16 cell factories

Cited by 11 publications

References 131 publications

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions

From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing

Advances in Defect Engineering of Metal Oxides for Photocatalytic CO2 Reduction

Contact Info

Product

Resources

About

Advances in Defect Engineering of Metal Oxides for Photocatalytic CO₂ Reduction