Problems Concerning “Noncultivable” Mycoplasma Contaminants in Tissue Cultures

Hopps, Hope E.; Meyer, Barbara C.; Barile, Michael F.; DelGiudice, Richard A.

doi:10.1111/j.1749-6632.1973.tb45655.x

Cited by 81 publications

(20 citation statements)

References 13 publications

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“…The finding of Gottlieb et a1 (12) that RA patients as opposed to control patients had experienced greater exposure to household pets should stimulate work to detect the possible presence of feline and canine species of mycoplasma in RA. T h e fact that none of these species has been isolated from RA patients could be due to the ability of some mycoplasmas to enter into a nonisolable phase in association with host cells (14). Unfortunately present mycoplasma media remain unsuitable for maintaining the growth of many fresh isolates (35).…”

Section: Discussionmentioning

confidence: 99%

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Studies on the infectious etiology of human rheumatoid arthritis

Ward

1975

Arthritis & Rheumatism

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A total of 29 rheumatoid patients and 19 nonrheumatoid patients were tested for evidence of present or past infection b y M pneumoniae, M hominis, M fermentam, M arthritidis, M pulmonis, and M hyorhinis. T h e techniques of lymphocyte transformation, metabolic-inhibiting antibody test, and mycoplasmacidal antibody test indicated no significant difference in the response of rheumatoid as opposed to nonrheumatoid patients.Although a wide variety of immunologic processes are apparent within the joints of patients exhibiting rheumatoid arthritis (RA), the nature of the initiating agent or event appears elusive. An infectious etiology for human rheumatoid arthritis remains an attractive hypothesis despite numerous conflicting and inconclusive results on the isolation of an agent.

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Section: Discussionmentioning

confidence: 99%

“…Additional evidence for the infectious etiology of human RA was recently presented by Gottlieb et al (12), who demonstrated that prior to onset of arthritis (14). T h u s a similar adaptation of a n agent to growth in h u m a n synovium may negate attempts to detect it.…”

mentioning

confidence: 97%

Studies on the infectious etiology of human rheumatoid arthritis

Ward

1975

Arthritis & Rheumatism

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“…There are some important, pathogenic mycoplasmas that grow poorly or not at all in artificial media and therefore can be grown and studied only in in vivo or cell culture systems (4,5,12,13,19). Moreover, even when the organisms can be grown axenically, adjustment to these conditions can influence the phenotypic and genomic properties and activities of the mycoplasma under study.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Mycoplasma hyorhinis DNA in the presence of host cells without growing the mycoplasma axenically

et al. 1991

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Mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. Since both mitochondria and mycoplasmas synthesize DNA by using the prokaryotic DNA polymerase gamma, the use of aphidicolln, which inhibits eukaryotic DNA polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial DNA. The restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both DNAs. Thus, it is possible to study the molecular biology of this noncultivable mycoplasma in situ rather than after growth in artificial media, with its potential genetic consequences during adjustment to axenic growth.

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“…Hayflick and Stanbridge (1967) found that 70% of mycoplasmas isolated from human, clinical material could not be sub-cultivated from agar plate colonies. Hopps et al (1973) identified Mycoplasma hijorhinis in cell cultures by immunofluorescence, though they failed to culture it on agar medium. Similar results were reported by Zgorniak-Nowosielska et al (1967), Schneider et al (1974b) and House and Waddell (1967).…”

Section: Discussionmentioning

confidence: 99%