Problems in the Identification of Species of Eimeria

Long, P. L.; Joyner, L. P.

doi:10.1111/j.1550-7408.1984.tb05498.x

Cited by 139 publications

(83 citation statements)

References 52 publications

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“…The epidemiology of avian coccidial parasites has been little investigated in detail due to the complete absence of methods that combine speed, reliability, objectivity, ease of use and the potential to discriminate between all species (Long & Joyner, 1984). In the present investigation, interspecific DNA sequence diversity within the ITS1 region on the rDNA from four pathogenic species of Eimeria was examined to reveal species-specific DNA sequences applicable for diagnostic use.…”

Section: Discussionmentioning

confidence: 99%

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic.Eimeriaspecies of the chicken

et al. 1998

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We describe a polymerase chain reaction (PCR)-based assay for the detection, identification and differentiation of pathogenic species of Eimeria in poultry. The internal transcribed spacer 1 (ITS1) regions of ribosomal DNA (rDNA) from Eimeria acervulina, E. brunetti, E. necatrix and E. tenella were sequenced and regions of unique sequences identified. Four pairs of oligonucleotide primers, each designed to amplify the ITS1 region of a single Eimeria species, were synthesised for use in the PCR assay. In tests on purified genomic DNA from all seven species of Eimeria that infect the chicken, each of the four primer pairs amplified the ITS1 region from only their respective target species. The robustness of the approach was further demonstrated by the amplification of specific DNA fragments from tissues of experimentally infected animals and from oocysts recovered from field samples. We conclude that the ITS1 regions of Eimeria species contain sufficient inter-specific sequence variation to enable the selection of primers that can be applied in PCR analyses to detect and differentiate between species. In future work they may provide excellent markers for epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic.Eimeriaspecies of the chicken

et al. 1998

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“…Moreover, egg hatching takes place under restricted conditions without any presence of circulating Eimeria species that might be able to infect these birds. This is probably due to host specificity (BIGLAND et al, 1965;EDGAR et al, 1964;SHAH & JOHNSON, 1971;LONG & MILLARD, 1979;FAYER, 1980;LONG & JOYNER, 1984). Furthermore, prophylactic use of anticoccidial drugs and biosecurity measures may also have strengthened the limitation of infection among these chukar partridges.…”

Section: Discussionmentioning

confidence: 99%

“…This species was found to be able to infect and develop its entire life cycle in Japanese quails (NORTON & PEIRCE, 1971). However, there are very few acceptable records of transmission of Eimeria species from one host genus to complete development in another, with the exception of situations that may reflect a close systematic relationship between two hosts (DORAN, 1978;LONG & JOYNER, 1984). In a similar situation, Japanese quails were infected in a laboratory with E. dispersa, which is known to be a parasite of partridges and grey quails, but not of chickens or turkeys (EDGAR et al, 1964).…”

Section: Discussionmentioning

confidence: 99%

Coccidia of gallinaceous meat birds in Brazil

Teixeira

Melo

Albuquerque

et al. 2015

Rev. Bras. Parasitol. Vet.

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Coccidiosis is a disease that limits the production and marketing of gallinaceous birds in North America, especially quails, pheasants and chukar partridges. Virtually no research has been conducted in South America on the causative agents of diseases among these birds, including coccidia. The aim of this work was to make first observations on Eimeria spp. in the chukar partridge Alectoris chukar and the grey quail Coturnix coturnix, which are reared for meat in Brazil. Fecal and tissue samples were collected from commercial farms and were examined for oocysts, gross and microscopic lesions or endogenous stages. From this examination, it was found that partridges raised in Brazil did not have any visible infection. However, grey quails presented mild infection and two Eimeria species that had previously been described in other birds were identified.Keywords: Eimeria, infection, diagnosis, oocyst, morphology. ResumoA coccidiose é uma enfermidade limitante para a produção e comercialização de aves de corte na América do Norte, principalmente codornas, faisões e a perdiz de chukar. Praticamente nenhuma pesquisa foi realizada na América do Sul sobre os agentes causadores de doenças nessas aves, incluindo coccídios. O objetivo deste trabalho foi realizar as primeiras observações sobre Eimeria spp. em perdiz de chukar Alectoris chukar e codornas cinzentas Coturnix coturnix criadas para abate no Brasil. Amostras de fezes e de tecidos foram coletadas em granjas comerciais e examinadas para oocistos, lesões macroscópicas e microscópicas ou estágios endógenos. Após o exame, verificou-se que a criação de perdizes, no Brasil, não tinha infecção visível. No entanto, as codornas cinzentas apresentaram uma infecção leve e foram identificadas duas espécies de Eimeria descritas anteriormente em outras aves.Palavras-chave: Eimeria, infecção, diagnóstico, oocisto, morfologia.

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“…Species identification is classically performed using morphometry, pre-patent period, sporulation time, lesion scoring techniques based on severity, intestinal site and shape of the lesions and characteristics of the endogenous stages in the intestinal mucosa (Joyner and Long 1974;Long 1982). However, these techniques require highly trained personnel and are limited by the overlap of characteristics among different species (Long and Joyner 1984). Furthermore, mixed infections can pose a problem for the precise determination of the intestinal site of the lesions (Jeffers 1975;McDougald and Jeffers 1976;Shirley et al 1984) has eliminated the pre-patent period as a good criterion for species identification.…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

et al. 2012

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About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4 9 11.2, 28.5 9 20.3, 31.1 9 18.5, 13.2 9 12.4, 20.8 9 17.5 and 22 9 18 lm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539 bp (E. tenella) and 460 bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.

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Problems in the Identification of Species of Eimeria

Cited by 139 publications

References 52 publications

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic.Eimeriaspecies of the chicken

Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic.Eimeriaspecies of the chicken

Coccidia of gallinaceous meat birds in Brazil

Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

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