Problems of Typing Dengue Viruses

Wm, Hammont; Ge, Sather

doi:10.1093/milmed/129.2.130

Cited by 16 publications

(7 citation statements)

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“…At the time, typing sera were prepared using multiple inoculations of DENV mouse brain suspensions in mice, hamsters, guinea pigs, rabbits, or monkeys. CF and neutralizing antibodies prepared in immunized mice or DENV-infected monkeys were successful in distinguishing DENV 3 and 4 from DENV 1 and 2 (38). However, the same test systems found evidence for the existence of DENV 5 and 6.…”

Section: Solving Technical Problems: Improved Laboratory Methodsmentioning

confidence: 86%

“…It was observed that DENV 1 and DENV TH-Sman (DENV 5) and DENV 2 and TH-36 (DENV 6) were closely related using CF tests of viral antigens with mouse sera. Sera recovered from infected humans often reacted with either DENV 5 or 6 more strongly than they did with prototype DENV 2 or 1, respectively (38). Over the next few years, the introduction of tissue culture neutralization tests and, partic- …”

Section: Solving Technical Problems: Improved Laboratory Methodsmentioning

confidence: 99%

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Dengue Hemorrhagic Fever at 60 Years: Early Evolution of Concepts of Causation and Treatment

Halstead

Cohen

2015

Microbiol Mol Biol Rev

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SUMMARY During the decade of the 1960s, the epidemiology of a new dengue disease, dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS), was described by collaborative research performed by Thai scientists from many institutions and by workers at the U.S. Army's SEATO Medical Research Laboratory in Bangkok, Thailand. Careful clinical and physiological studies provided the initial description of DSS. DSS cases were caused by each of the four dengue viruses (DENV) and not chikungunya (CHIK) virus or DENV 5 and 6, were associated with a secondary-type dengue antibody response in children over the age of 1 year, were associated with a primary antibody response in infants less than 1 year old whose mothers had neutralizing antibodies to all four DENV, were associated more frequently with secondary DENV 2 infections than those due to DENV 1 and 3, and were more common in females than males over the age of 3 years. Robust laboratory methods for growth and recovery of DENV in tissue cultures were introduced. In addition, life-saving principles of fluid and plasma protein resuscitation of hypovolemia were described. Most epidemiological observations made during the decade of the 1960s have been confirmed in the succeeding 45 years. Much contemporary research on pathogenesis fails to address the two distinct immunological antecedents of DHF/DSS.

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Section: Solving Technical Problems: Improved Laboratory Methodsmentioning

confidence: 86%

Section: Solving Technical Problems: Improved Laboratory Methodsmentioning

confidence: 99%

Dengue Hemorrhagic Fever at 60 Years: Early Evolution of Concepts of Causation and Treatment

Halstead

Cohen

2015

Microbiol Mol Biol Rev

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“…In 1964, Hammon and Sather9 wrote on the difficulties of typing DENV caused by serologic cross-reactivity and antigenic similarities. At that time, three assays were being used to distinguish DENV types: virus neutralization using a suckling mouse model, complement fixation, and hemagglutination inhibition.…”

Section: Introductionmentioning

confidence: 99%

Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance

Thomas¹,

Nisalak²,

Anderson³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

196

160

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Abstract. Dengue virus (DENV) infection is a worsening global health problem. The plaque reduction neutralization test (PRNT) is currently considered to be the "gold standard" to characterize and quantify circulating levels of anti-DENV neutralizing antibody (NAb). Many variations of the PRNT are currently in use and neither the assay nor its performance conditions have been standardized or harmonized between laboratories. We used a well-characterized panel of acute and late convalescent follow-up sera samples from children experiencing primary and secondary DENV infections to evaluate the performance of the dengue PRNT under a variety of testing conditions. Investigators varied cell type, control virus passage, and the use of complement across multiple assay runs of the same sample panel. Our findings indicate wide variation in PRNT titer results in response to varied testing conditions.

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“…Haemagglutination-inhibition is the least specific of the sérodiagnostic techniques 19 and positive titers imply little more than infection with a group virus. Haemagglutination-inhibition is the least specific of the sérodiagnostic techniques 19 and positive titers imply little more than infection with a group virus.…”

Section: Commentmentioning

confidence: 99%

Three Faces of Dengue

DOOLEY¹

1966

Arch Intern Med

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Problems of Typing Dengue Viruses

Cited by 16 publications

References 0 publications

Dengue Hemorrhagic Fever at 60 Years: Early Evolution of Concepts of Causation and Treatment

Dengue Hemorrhagic Fever at 60 Years: Early Evolution of Concepts of Causation and Treatment

Dengue Plaque Reduction Neutralization Test (PRNT) in Primary and Secondary Dengue Virus Infections: How Alterations in Assay Conditions Impact Performance

Three Faces of Dengue

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