Procedure 27 Electrochemical detection of calf thymus double-stranded DNA and single-stranded DNA by using a disposable graphite sensor

Gulmez, Beste; Karadeniz, Hakan; Erdem, Arzum; Ozsoz, Mehmet

doi:10.1016/s0166-526x(06)49070-x

Cited by 5 publications

(4 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PGEs were pretreated by applying þ1.40 V for 30 s in ABS without stirring; 16 mg/mL concentration level of dsDNA was immobilized onto the pretreated PGEs by following the dip-coating procedure for 7.5 min. 20,21 After immobilization of DNA onto PGEs, each PGE was then rinsed with ABS for 10 s.…”

Section: 34mentioning

confidence: 99%

New tetracyclic heteroaromatic compounds based on dehydroamino acids: photophysical and electrochemical studies of interaction with DNA

et al. 2008

Self Cite

View full text Add to dashboard Cite

A benzothienoindole (BTIN) and a benzofuroindole (BFIN) were synthesized in high yields, as potential new DNA target compounds, using a metal-assisted intramolecular CeN cyclization of the methyl esters of N-(tert-butoxycarbonyl)-b,b-bis(dibenzothien-4-yl or dibenzofur-4-yl)dehydroalanines. The latter were obtained by a bis-Suzuki coupling of a b,b-dibromodehydroalanine with the corresponding heteroarylboronic acids. The absorption and fluorescence properties of the new tetracyclic compounds were studied in different solvents and in the presence of salmon sperm DNA. The results in several solvents show that either BTIN or BFIN can be used as fluorescence solvent sensitive probes. Spectroscopic studies of their interaction with dsDNA allowed to determine binding constant (K i) values and binding site sizes (n). Fluorescence quenching experiments using iodide ion showed that intercalation is the preferred mode of binding to DNA. From the results obtained, BTIN is the more intercalative compound and has a higher affinity to DNA. The interaction of BTIN with DNA was also studied electrochemically, by using differential pulse voltammetry (DPV) in connection with disposable pencil graphite electrode (PGE). After the interaction of BTIN with DNA, the oxidation signals of BTIN and adenine strongly decreased. The latter result was attributed to the binding of BTIN to DNA and the former points to a possible damage of the oxidizable groups of BTIN after intercalation into DNA. The results of spectroscopic and electrochemical studies of BTIN interaction with DNA are in good agreement.

show abstract

Section: 34mentioning

confidence: 99%

New tetracyclic heteroaromatic compounds based on dehydroamino acids: photophysical and electrochemical studies of interaction with DNA

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…38 The electrode was then rinsed with ABS for 10 s. The dsDNAmodified PGE was then immersed into the solution of 10 µ g/mL each compound (C1/C2/C3/C4) prepared in PBS (pH 7.40). Each electrode was then rinsed with PBS for 10 s. The control experiments were done in the absence of compounds or DNA.…”

Section: Interaction Of Surface-confined Dna With Drugs By Pgementioning

confidence: 99%

Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes

Istanbullu¹,

Karadeniz²,

Erciyas³

et al. 2017

Turk J Chem

Self Cite

View full text Add to dashboard Cite

Four different Mannich base derivatives containing an aromatic/heteroaromatic propanone structure (C1 (3-(dimethylamino)-1-(thiophen-2-yl)propan-1-one hydrochloride); C2 (3-morpholino-1-(pyridin-3-yl)propan-1-one hy-propan-1-one hydrochloride)) were earlier synthesized and characterized with 1 H NMR, 13 C NMR, MS, and elemental analysis. The interaction of these compounds with fish sperm double stranded DNA (fs-dsDNA) was investigated by using differential pulse voltammetry (DPV) in connection with a disposable pencil graphite electrode (PGE). After the interaction procedure, there was a meaningful decrease in the oxidation signal of the electroactive DNA base guanine due to possible intercalation and/or alkylation mechanism between these Mannich base derivatives and DNA. The features of this electrochemical assay were discussed in comparison to previous reports related to DNA targeted agents/drug candidates in the literature.

show abstract

“…The pretreated PGEs were immersed into the vials containing either 110 mL of samples containing DNA, or KA incubated DNA. The electrodes were then kept in these vials during 7.5 min followed by passive adsorption procedure [12,14,36]. The PGEs were washed with ABS for 10 s.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

“…The electrochemical detection of nucleic acids immobilized onto different transducers by adsorption, crosslinking, complexation and covalent attachment was performed with the intention of detecting DNA hybridization and DNA interactions [13,14]. Electrochemical analysis of DNA also offers a number of approaches in DNA damage detection as well as in sensing of DNA damaging agents in the environment, in food in clinical samples, etc.…”

Section: Introductionmentioning

confidence: 99%

The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid

et al. 2009

Self Cite

View full text Add to dashboard Cite

A possible DNA damage after interaction of kainic acid (KA) with calf thymus double stranded DNA and genomic DNA was herein determined in in vitro and in vivo conditions using; electrochemical assay and agarose gel electrophoresis. The changes in guanine signal were detected as an indicator of DNA damage in genomic DNA samples isolated from 1 or 10 mg/kg KA-treated animals. The decreased levels of guanine signal were found as 29% and 33% by 1 and 10 mg/kg KA treatment when compared to controls, respectively. The results of gel electrophoresis confirmed DNA damage obtained in identical samples by electrochemical method.

show abstract

Procedure 27 Electrochemical detection of calf thymus double-stranded DNA and single-stranded DNA by using a disposable graphite sensor

Cited by 5 publications

References 12 publications

New tetracyclic heteroaromatic compounds based on dehydroamino acids: photophysical and electrochemical studies of interaction with DNA

New tetracyclic heteroaromatic compounds based on dehydroamino acids: photophysical and electrochemical studies of interaction with DNA

Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes

The Comparison of Electrochemical Assay and Agarose Gel Electrophoresis for the Determination of DNA Damage Induced by Kainic Acid

Contact Info

Product

Resources

About