Proceedings of the International Symposium on Plasmid Biology

Andrzejewska, Joanna; Lee, Sae Kyun; Olbermann, Patrick; Lotzing, Nina; Katzowitsch, Elena; Linz, Bodo; Achtman, Mark; Kado, Clarence I.; Suerbaum, Sebastian; Josenhans, Christine

doi:10.1016/j.plasmid.2006.12.001

Cited by 3 publications

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“…RepABC family replicon. We have previously described the obtention of a partial library of pSmeLPU88b plasmid [ 42 , 54 ]. One clone of the library, pG700, contained a DNA fragment encoding the C-terminal domain of the RepC of the repABC operon in pG18mob2 plasmid (non-replicative in rhizobia).…”

Section: Methodsmentioning

confidence: 99%

Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

et al. 2023

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Rhizobia are Gram-negative bacteria known for their ability to fix atmospheric N2 in symbiosis with leguminous plants. Current evidence shows that rhizobia carry in most cases a variable number of plasmids, containing genes necessary for symbiosis or free-living, a common feature being the presence of several plasmid replicons within the same strain. For many years, we have been studying the mobilization properties of pSmeLPU88b from the strain Sinorhizobium meliloti LPU88, an isolate from Argentina. To advance in the characterization of pSmeLPU88b plasmid, the full sequence was obtained. pSmeLPU88b is 35.9 kb in size, had an average GC % of 58.6 and 31 CDS. Two replication modules were identified in silico: one belonging to the repABC type, and the other to the repC. The replication modules presented high DNA identity to the replication modules from plasmid pMBA9a present in an S. meliloti isolate from Canada. In addition, three CDS presenting identity with recombinases and with toxin-antitoxin systems were found downstream of the repABC system. It is noteworthy that these CDS present the same genetic structure in pSmeLPU88b and in other rhizobial plasmids. Moreover, in all cases they are found downstream of the repABC operon. By cloning each replication system in suicide plasmids, we demonstrated that each of them can support plasmid replication in the S. meliloti genetic background, but with different stability behavior. Interestingly, while incompatibility analysis of the cloned rep systems results in the loss of the parental module, both obtained plasmids can coexist together.

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Section: Methodsmentioning

confidence: 99%

Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

et al. 2023

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Historical Events That Spawned the Field of Plasmid Biology

Kado

2015

Plasmids

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Historical Events That Spawned the Field of Plasmid Biology

Kado

2014

Microbiol Spectr

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This chapter revisits the historical development and outcome of studies focused on the transmissible, extrachromosomal genetic elements called plasmids. Early work on plasmids involved structural and genetic mapping of these molecules, followed by the development of an understanding of how plasmids replicate and segregate during cell division. The intriguing property of plasmid transmission between bacteria and between bacteria and higher cells has received considerable attention. The utilitarian aspects of plasmids are described, including examples of various plasmid vector systems. This chapter also discusses the functional attributes of plasmids needed for their persistence and survival in nature and in man-made environments. The term plasmid biology was first conceived at the Fallen Leaf Lake Conference on Promiscuous Plasmids, 1990, Lake Tahoe, California. The International Society for Plasmid Biology was established in 2004 (www.ISPB.org).

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Proceedings of the International Symposium on Plasmid Biology

Cited by 3 publications

References 0 publications

Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

Historical Events That Spawned the Field of Plasmid Biology

Historical Events That Spawned the Field of Plasmid Biology

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