Proceedings: Using Stem Cell Therapies to Reestablish Osteogenic Capability for Bone Regeneration

Littman, Neil; Abo, Arie

doi:10.5966/sctm.2015-0202

Cited by 4 publications

(3 citation statements)

References 4 publications

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“…Mesenchymal stem cells are currently the most established promising cells for skeletal tissue engineering and regeneration ; however, the limited understanding of the mechanisms MSCs mediated hinders the clinical translation. The paracrine hypothesis has inspired an alternative approach in bone regeneration, which is based on EVs secreted by the stem or progenitor cells rather than cells themselves.…”

Section: Stem or Progenitor Cells‐derived Extracellular Vesicles Applmentioning

confidence: 99%

Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair

Zhao

et al. 2017

Stem Cells Translational Medicine

120

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SummaryStem cells, with their therapeutic potential in tissue repair and regeneration, have been widely used in translational medicine. Recent evidence suggests that the beneficial effects are mediated largely by their paracrine actions rather than the engraftment and differentiation at the injured sites. Extracellular vesicles (EVs), actively released from cells, play important roles in cell‐to‐cell communication and display multiple functions in tissue regeneration. In the present report, we will briefly review the current knowledge related to the therapeutic potential of EVs, particularly stem cell or progenitor cell‐derived ones for promoting tissue repair and regeneration, and focus on the restorative properties of exosomes/microvesicles in cutaneous wound healing, bone regeneration, hindlimb ischemia, and vascular injury repair. Stem Cells Translational Medicine 2017;6:1753–1758

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Section: Stem or Progenitor Cells‐derived Extracellular Vesicles Applmentioning

confidence: 99%

Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair

Zhao

et al. 2017

Stem Cells Translational Medicine

120

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“…Moreover, live-cell industrialization and preservation have also not yet been available for clinical application. Furthermore, cell therapy alone also failed due to its short shelf life (Littman and Abo, 2015). Low bone formation was observed in osteoporotic conditions due to scanty recruitment and invasion of MSCs to the defect bone for bone healing (Almeida et al, 2019;Sanghani-Kerai et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Therapeutic approach for osteoporotic animal model by bone marrow mesenchymal stem cells

Aljohani¹,

Zaki²,

Waleed³

et al. 2022

J App Pharm Sci

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Osteoporosis (OP) is a common debilitating metabolic bone disorder manifested by poor mineral density and deteriorated skeleton. Stem cell therapies are being considered as alternative therapeutic modalities. This study determined the therapeutic potential of mesenchymal stem cell (MSC) application in osteoporotic rats. To achieve this, an OP animal model was developed through ovariectomy and glucocorticoids in rats. MSCs were isolated from the same breed of rats and cultured MSCs were administered to osteoporotic rats via vein injections. After 4 and 8 weeks of MSCs administration, rats were sacrificed and all desired components, including serum and joints, were isolated. The present findings showed that the transplantation of MSCs into osteoporotic rats enhanced osteoblastic differentiation and function reverting OP. Calcium deposition, bone-specific alkaline phosphatase activity, and serum osteocalcin concentration were significantly elevated in MSCs-treated osteoporotic rats (p < 0.05). Notably, their levels were much higher in osteoporotic rats treated for 8 weeks than in those treated for 4 weeks (p < 0.05). Moreover, the histological image of the femur head showed uniform thinning of trabeculae (TB) in osteoporotic rats, resulting in the widening of intertrabecular spaces and disjointed bony ossicles interrupted by virtue of expanded fatty bone marrow spaces. Interestingly, after MSCs treatment, cancellous bone TB appeared normal with red bone marrow. Furthermore, a photomicrograph of osteogenic differentiation of MSCs of MSCs-treated osteoporotic rats showed significantly high scattered red calcified nodules (p < 0.05). In conclusion, this study illustrates that the transplantation of MSCs into osteoporotic rats provides a supplement for bone architecture and enhances osteoblastic differentiation and function, thus reverting OP. This work presents a unique method as a future application for the treatment of human OP.

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“…However, the manufacturing and preservation of live cells are not facile for clinical application. Also, cell therapy normally does not offer off‐the‐shelf capability …”

Section: Introductionmentioning

confidence: 99%

Injection of synthetic mesenchymal stem cell mitigates osteoporosis in rats after ovariectomy

Shen

Jin

et al. 2018

J Cellular Molecular Medi

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Osteoporosis is a severe skeletal disorder. Patients have a low bone mineral density and bone structural deterioration. Mounting lines of evidence suggest that inappropriate apoptosis of osteoblasts/osteocytes leads to maladaptive bone remodelling in osteoporosis. It has been suggested that transplantation of stem cells, including mesenchymal stem cells, may alter the trajectory of bone remoulding and mitigate osteoporosis in animal models. However, stem cells needed to be carefully stored and characterized before usage. In addition, there is great batch‐to‐batch variation in stem cell production. Here, we fabricated therapeutic polymer microparticles from the secretome and membranes of mesenchymal stem cells (MSCs). These synthetic MSCs contain growth factors secreted by MSCs. In addition, these particles display MSC surface molecules. In vitro, co‐culture with synthetic MSCs increases the viability of osteoblast cells. In a rat model of ovariectomy‐induced osteoporosis, injection of synthetic MSCs mitigated osteoporosis by reducing cell apoptosis and systemic inflammation, but increasing osteoblast numbers. Synthetic MSC offers a promising therapy to manage osteoporosis.

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Proceedings: Using Stem Cell Therapies to Reestablish Osteogenic Capability for Bone Regeneration

Cited by 4 publications

References 4 publications

Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair

Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair

Therapeutic approach for osteoporotic animal model by bone marrow mesenchymal stem cells

Injection of synthetic mesenchymal stem cell mitigates osteoporosis in rats after ovariectomy

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