Life Cycle Assessment (LCA) is an organized and objective methodology for determining potential impacts and environment aspects related with service or product. Besides determining potential environment impacts, LCA potentially becomes a powerful decision maker tools to entrepreneur in industry. Nowadays, biotechnology industry consider as high potential, high risk and high return industry (3H), so that entrepreneurs in biotechnology need a very powerful, systematic, data based tool during decision making. In this review, the discussion focused on the introduction of LCA, the potential as decision maker and the beneficial potential of LCA in biotechnology industry and environment aspects.