Process and Laboratory Scale UV Inactivation of Viruses and Bacteria Using an Innovative Coiled Tube Reactor

Abstract

Avoidance and elimination of viral contamination in biotechnological and pharmaceutical processes is a crucial issue. The novel UVivatec coiled tube reactor was designed for UV irradiation of cloudy liquid media. It very effectively inactivates especially critical viruses and avoids high product losses. The method proposed is an ideal complement to existing and established processes.

Treating cell culture media with UV irradiation against adventitious agents: Minimal impact on CHO performance

“…5 Ultraviolet (UV) radiation is known to be an effective sterilant against various biological contaminants, including bacteria and viruses. [11][12][13][14] For example, active cytomegalovirus (CMV), can be reduced by one log with a UV fluence of 5 mJ/cm 2,15 while minute virus of mice (MVM) requires only 2 mJ/cm 2 . 16,17 More resistant viruses, such as adenovirus require up to 306 mJ/cm 2 to achieve a log inactivation of approximately 6.…”

Study of short-wave ultraviolet treatments (UV-C) as a non-thermal preservation process for liquid egg products

“…In this study, the UV-C treatment devices are based on liquid flow in a coiled tube that causes secondary eddy flow effects (Dean Vortices), also known as the Dean effect (Dean, 1927;Schmidt and Kauling, 2007). …”

Botryococcus braunii for the production of hydrocarbons and exopolysaccharides and the role of associated bacteria

“…UV is a preferred method of sterilization for bacteria and viruses because of its mode of action being nonthermal and non-adulterating (Yen et al 2014). It is widely used in pharmaceutical industry and water purification systems (Abshire 1988, Schmidt andKauling 2007). UVC nm) is the UV range used to inactivate microorganisms by damaging the genetic material inside the nucleus (Dai et al 2012).…”

“…UVC damages the genetic material in the nucleus of the bacteria specially between 250 -270 nm as this is the wavelength that is most strongly absorbed by nucleic acids and therefore the most lethal range of wavelength to microorganisms and is known as the "germicidal spectrum" (Dai et al 2012). In the pharmaceutical industry for example, laboratory scale UVC treatment is being applied to inactivate viruses and bacteria in cloudy liquid media (Schmidt and Kauling 2007).…”

“…Several approaches are commonly used such as, centrifugation, washing and sieving methods, and the use of antibiotics (Bruckner and Kroth 2009, Han et al 2016. Another method of sterilization commonly used in environmental technology, medical practices and pharmaceutical industry is the use of UV (Schmidt and Kauling 2007, Carney and Lane 2014, Yen et al 2014. Ultra-violet light C (UVC) which is in the range of 200 -280 nm, damages the genetic material in the nucleus especially the range between 250 -270 nm which is strongly absorbed by nucleic acids (Dai et al 2012).…”