Process engineering strategy for high cell density-lipid rich cultivation of Chlorella sp. FC2 IITG via model guided feeding recipe and substrate driven pH control

Palabhanvi, Basavaraj; Muthuraj, Muthusivaramapandian; Mukherjee, Mayurketan; Kumar, Vikram; Das, Debasish

doi:10.1016/j.algal.2016.03.024

Cited by 19 publications

(4 citation statements)

References 44 publications

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“…In the second phase, high lipid content was obtained under nitrogen depletion conditions (higher C/N ratios). In this study, lipid productivity attained 2-to 5-fold increase compared to other studies [166][167][168].…”

Section: Combined Cultivation Strategiesmentioning

confidence: 44%

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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Microalgae have received widespread interest owing to their potential in biofuel production. However, economical microalgal biomass production is conditioned by enhancing the lipid accumulation without decreasing growth rate or by increasing both simultaneously. While extensive investigation has been performed on promoting the economic feasibility of microalgal-based biofuel production that aims to increase the productivity of microalgae species, only a handful of them deal with increasing lipid productivity (based on lipid contents and growth rate) in the feedstock production process. The purpose of this review is to provide an overview of the recent advances and novel approaches in promoting lipid productivity (depends on biomass and lipid contents) in feedstock production from strain selection to after-harvesting stages. The current study comprises two parts. In the first part, bilateral improving biomass/lipid production will be investigated in upstream measures, including strain selection, genetic engineering, and cultivation stages. In the second part, the enhancement of lipid productivity will be discussed in the downstream measure included in the harvesting and after-harvesting stages. An integrated approach involving the strategies for increasing lipid productivity in up- and down-stream measures can be a breakthrough approach that would promote the commercialization of market-driven microalgae-derived biofuel production.

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Section: Combined Cultivation Strategiesmentioning

confidence: 44%

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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“…With successful cultivation using the techniques above, it is possible to obtain a high-density culture that is unobtainable under photoautotrophic modes, which allows economic harvesting and easier downstream processing for large-scale production 13 . In some cases, fed-batch cultivation can achieve culture density of over 70 g/L, far exceeding that of the photoautotrophic cultures 8 and considering that centrifugation which is one of the common harvesting methods brings about 100 g/L cell density, then it surely saves the costs for the harvests 14,15 . Lastly, heterotroph based massive cultivation could make it possible to produce value-added products which have higher prices at least greater more than biofuel 9,16–18 .…”

Section: Introductionmentioning

confidence: 99%

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Kim

Park

Lee

et al. 2019

Sci Rep

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The heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a three-fold improvement in biomass amounts, from 5.85 to 18.13 g/L, in comparison to a non-optimized BG11 medium containing 72 g/L glucose. Scaling up the cultivation to a 5-L fermenter resulted in a greatly improved biomass concentration of 35.3 g/L owing to more efficient oxygenation of the culture. In addition, phosphorus feeding fermentation was employed in an effort to address early depletion of phosphate, and a maximum biomass concentration of 42.95 g/L was achieved, with biomass productivity of 5.37 g/L/D.

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“…Acetic acid or acetate is also known as lipid inducer since the lipid content is usually higher than glucose-fed cultures [21,28,29].…”

Section: Lipid Productionmentioning

confidence: 99%

“…Still, in most cases, glucose resulted higher total biomass and productivity [26,27]. Acetate has been worked effectively as an inducer of lipid production [28,29] and also in terms of biomass yield [19]. At the same time it can be easily assimilated by the cells through the glyoxylate cycle or the tricarboxylic acid cycle (TCA) in the form of acetyl coenzyme A [30].…”

Section: Introductionmentioning

confidence: 99%

Effect of Fed-batch Culturing on the Growth and Lipid Production of Chlorella vulgaris fo. tertia Applying pH-auxostat Acetic Acid and Predefined Exponential Glucose Feeding

Nagy

Ivanics

et al. 2022

Period. Polytech. Chem. Eng.

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The techniques of heterotrophic microalgae cultivation used to be resulting in higher productivity and better yield than autotrophic culturing. Batch cultivation strategy is commonly used with high glucose concentration, but its potential is limited for biomass production at an industrial scale. Usually, the best productivity can obtain at lower glucose concentration. Moreover, other carbon sources can cause inhibition at higher concentrations. Therefore, the fed-batch cultivation strategy is an obvious choice, as it can maintain the optimal amount of carbon source can be maintained throughout the fermentation by automating the feeding. Such self-regulatory automation is provided by the pH-auxostat addition of acetic acid, which was investigated in this study for Chlorella vulgaris fo. tertia. The pH-auxostat fermentation was upscaled, then the feeding profile was modelled and transformed to another fermentation where glucose was used as a carbon source instead of acetic acid. Thus, the preferred carbon sources were compared under the same circumstances. It was found that the tested strain consumes dissolved oxygen very fast on both carbon substrates. It favored the acetic acid at high nitrogen and phosphorus concentrations. The final biomass concentration was 29.2 g/L under pH-auxostat fed-batch strategy with acetic acid and 18.8 g/L with glucose, respectively. The highest lipid content (393 mg/g) was measured from the biomass in the case of acetic acid. The fermentation settings need further optimization, but the results concluded that pH-auxostat acetic acid feeding has a great potential for scale-up of Chlorella fermentation.

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Process engineering strategy for high cell density-lipid rich cultivation of Chlorella sp. FC2 IITG via model guided feeding recipe and substrate driven pH control

Cited by 19 publications

References 44 publications

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

Effect of Fed-batch Culturing on the Growth and Lipid Production of Chlorella vulgaris fo. tertia Applying pH-auxostat Acetic Acid and Predefined Exponential Glucose Feeding

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