Process evaluation of electron beam irradiation-based biodegradation relevant to lignocellulose bioconversion

Abstract

In order to solve the inefficient problem of long-term biodegradation by wood-decaying fungus, rice straw (RS) was depolymerized using electron beam irradiation-based biodegradation (EBIBB). This environment-friendly program without the use of inhibitory byproducts significantly increased the digestibility and fermentability of RS. Specifically, when irradiated RS was simultaneously biodegraded by Phanerochaete chrysosporium for 10 days, the sugar yield was 65.5% of the theoretical maximum. This value was on the same level as the 64.8% (for 15 days) measured from unirradiated RS. In case of fermentability, similarly, EBIBB program had an effect on time/energy saving. Furthermore, the transcriptomic profiles under different biosystem were analyzed in order to verify possible substrate-specific regulation based on change of lignocellulosic components. Interestingly, the overall correlation based on the bias (upregulation or downregulation) was reasonably analogous, especially lignocellulolysis-related genes.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-3-487) contains supplementary material, which is available to authorized users.

Bioprocess Evaluation of Water Soaking-Based Microbiological Biodegradation with Exposure of Cellulosic Microfibers Relevant to Bioconversion Efficiency

“…2). Interestingly, independent of either water-soaking preprocess or crystalline modification (e.g., CrI; Table 3), the general profile of major proteins involved in intracellular signaling and metabolic maintenance was significantly downregulated (Table 2), similar to that previously reported in a nonsoaking biosystem [8,27]. Under harsh conditions with recalcitrant substrates, extravagant pathways, which involve the activation of lignocellulolytic targets, are not expected be initiated at the inactive cells, probably due to the optimized self-complementation mechanism (Tables 2 and 3) to achieve time/energy Fig.…”

“…However, the extracellular bioplatform with conserved stability did not yet rule out the minor triggers, which draw the actual possibility on either process stability or recuperative strength (Table 3). Interestingly, the inevitable targets in the extracellular platform for maintaining lignocellulolytic biocascades can be spontaneously and simultaneously activated, regardless of the uniform exposure of nonamorphous fibers [8,27]. The driving loss of three key components, as well as the rising trend of industrial yields, supported the powerful action of practical extracellular networks (Tables 1 and 4).…”

A Critical Look at Bioproducts Co-cultured Under Solid State Fermentation and Their Challenges and Industrial Applications

“…Even slight variations in the co-culturing system destabilize their synergistic balance and modify the behavior of the organisms which eventually leads to loss of product. Identifying the metabolites, proteins, and peptides released by all the organisms within a co-culture helps to understand the population dynamics and underlying communication to develop a successful industrial consortium [67].…”

“…The nature of the substrate greatly affects the choice of the pretreatment method. Physical methods include grinding, milling, densification, irradiation, and high temperature which can improve product yields by increasing the available surface area and size of pores [65][66][67]. Chemical type of pretreatment widely involves the use of acids, alkali, ammonia, and related methods [68].…”

Advanced Bioethanol Production: From Novel Raw Materials to Integrated Biorefineries

“…Advanced pretreatments are designed to overcome the limitations of conventional pretreatments. For this purpose, they employ novel solvents (i.e., super-critical fluids [57], ionic liquids (ILs) [58], deep eutectic solvents (DESs) [59], inorganic salts [60]), novel technologies (i.e., popping [61], gamma ray [62], electron beam irradiation [63]), novel process strategies such as milder operating conditions and/or higher solids content (i.e., low liquid ammonia [64], low-temperature hot water pretreatment [65]), or a combination of several principles (see Section 3.1.4). This type of pretreatment presents one or more advanced features such as being carried out at milder operation conditions, not producing inhibitors, low chemical usage and high specificity towards lignin or efficient fractionation [54,66].…”