“…However, the unacceptable high cost of the vegetable oil namely soy bean, palm, and rapeseed, which account for about 70-90% of the total production cost (Vicente et al, 2009;Xue et al, 2008) is the major bottleneck for its broader application. One of the alternative ways to produce biodiesel in a green and sustainable manner, without competing with food crops, is the cultivation of oleaginous microorganisms, such as yeast, fungi, bacteria, and microalgae (Ryu et al, 2013;Thiru et al, 2011;Xu et al, 2013). Previous research showed that these microbes have several advantages in comprison to those vagetable oil and animal fats, such as high production rate, limited land-use, easy scale-up, and negligible effect of climate changes (Thiru et al, 2011).…”