Process Optimization and Characterization of Manila Tamarind Seed Oil Extracted by the Soxhlet Method

Sekhar, S. Chandra; Karuppasamy, K.; Vedaraman, N.; Sathyamurthy, Ravishankar; Vijayabalan, P.; Elshiekh, Ammar H.; Vinayagam, Nadanakumar; Manokar, A. Muthu

doi:10.1615/interjenercleanenv.2020034890

Cited by 7 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The seed extract preparation was carried out in the same manner as that described by S. Chandra Sekhar et al [25] with the aforementioned improvements. The air-dried pulverized seed content of P. dulce was sieved to mesh size 40.…”

Section: Preparation Of Extractmentioning

confidence: 99%

9-Hexadecenoic Acid Rich HPLC Fraction of Pithecellobium dulce Methanolic Seed Extract Exhibits Potential Antiinflammatory Activity by Inhibiting IL-8, IL-6, and PGE2: Phytochemical Characterization, in-vitro and in-vivo Evaluation

YELUGUDARI,

MESRAM,

KARNATI

2023

jrp

View full text Add to dashboard Cite

Pithecellobium dulce (Roxb.) Benth. has been used as a folk medicine due to its wide range of pharmacological applications. In light of the lack of significant studies examining the anti-inflammatory potential of seeds and their phytochemical constituents, we investigated the molecular basis behind the therapeutic efficacy of methanolic extract both in-vitro and in-vivo. The phytochemical investigation of methanolic seed extract using GC-MS analysis revealed the presence of 30 molecules of diverse classes. Further solvent fractionation followed by RP-HPLC separation demonstrated the presence of 9-Hexadecenoic acid and 8,11,14 (Z, Z, Z)-Eicosatrienoic acid and were further structurally characterized by 1H-NMR and HRMS. 9-Hexadecenoic acid-rich HPLC fraction exhibited the highest % inhibition of albumin denaturation and % reduction of IL-8 and IL-6 in HaCaT cells as well as NO and PGE-2 production in RAW267.4 cells among other test samples. In-vivo anti-inflammatory findings revealed that 9-Hexadecenoic acidrich fraction potentially reduced rat paw edema by 84.62% at 150 µg/mL, which is superior to the diclofenac. The results of the present study revealed that the 9-hexadecenoic acid-rich fraction of methanolic seed extract of P. dulce displayed potential anti-inflammatory activity both in-vitro and in-vivo, which suggests an efficient alternative in treating a variety of ailments related to inflammation and pain.

show abstract

Section: Preparation Of Extractmentioning

confidence: 99%