Process Optimization by a Response Surface Methodology for Extraction of Anthocyanins from Violet Glutinous Rice Grown in Phu Tho Province, Vietnam

Abstract

Anthocyanins are naturally occurring polyphenols that plays an essential role in the prevention and treatment of cancer, eye diseases, and so on. This study aimed to determine the natural anthocyanins from violet glutinous rice grown in Phu Tho province, Vietnam based on its extracts and optimize the process with response surface methodology (RSM). Four experimental parameters including the material-solvent ratio (g/mL), time (minutes) and microwave power (W) was selected for optimization. The maximum yield of anthocyanin was 1,385 mg/g, achieved at conditions of material-to-solvent ratio of 10.29 g/mL, a time of 4.4 min, and a microwave power of 90.54W. The established RSM model showed relative good fit with the experimental data regarding the anthocyanin extraction process (R2= 0.988).

Effects of Various Processing Parameters on Polyphenols, Flavonoids, and Antioxidant Activities of Codonopsis javanica Root Extract

“…The selected experiment design was Central Composite Design, and the experimental matrix was built using Design-Expert 11.0 software. [28][29][30][31] Antioxidant Activity of CJR Extract Test for radical scavenging activity using the DPPH method. The extract was first diluted to a reasonable concentration.…”

“…The selected experiment design was Central Composite Design, and the experimental matrix was built using Design-Expert 11.0 software. [28][29][30][31]…”