Process performance improvement for the simultaneous production of ligninolytic enzymes in solid culture using agricultural wastes through the Taguchi method

Sosa-Martínez, Jazel Doménica; Balagurusamy, Nagamani; Benavente‐Valdés, Juan Roberto; Montañez, Julio; Morales‐Oyervides, Lourdes

doi:10.1016/j.jenvman.2021.112966

Cited by 11 publications

(2 citation statements)

References 36 publications

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“…central composite design (CCD) and Box-Behnken design (BBD). Recognized as a simple statistical tool, Taguchi technique has been widely applied in multiple studies [21][22][23] for the enhancement on protocol design and process efficacy.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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The oil palm biomass produced by plantations and mills in Malaysia is the largest contributor to the nation’s agro-waste, with the oil palm leaves (OPL) topping the list. Nevertheless, the surplus of OPL might have applicability as the substrate for cultivating lignocellulolytic bio-degraders. Ipso facto, this study employed raw OPL as the carbon source for cultivating Trichoderma harzianum under solid-state fermentation (SSF). Optimization of the SSF process using the Taguchi orthogonal design to produce endoglucanase (CMCase) successfully established the optimal fermentation conditions as the following: 7.00 × 108 spore/g inoculum size, 50% moisture content, pH 12 Mandel’s medium, with 3-day incubation at 40°C. The crude enzyme cocktail exhibited the corresponding maximum activity of 417.49 U/g CMCase. The enzymatic activities were significantly affected by factors, moisture content, inoculum size, and initial pH (p-value < 0.05). In short, the high extracellular CMCase activity of the T. harzianum crude enzyme cocktail may prove valuable in accelerating the saccharification of cellulose for biofuel-and nanocellulose production.

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Section: Introductionmentioning

confidence: 99%

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Teo¹,

Wahab

2023

J.Trop.Life.Science

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“…Horseradish peroxidase (from a plant) has also demonstrated the ability to degrade tetracycline, about 50% removal efficiency within 1 h [3]. Phanerochaete chrysosporium (one kind of white-rot fungi) is well known for being used in the removal of xenobiotic organic contaminates [14] due to the expression of three principal ligninolytic enzymes (manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase) [15].…”

Section: Introductionmentioning

confidence: 99%

Transformation of Tetracycline by Manganese Peroxidase from Phanerochaete chrysosporium

et al. 2021

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The negative impacts on the ecosystem of antibiotic residues in the environment have become a global concern. However, little is known about the transformation mechanism of antibiotics by manganese peroxidase (MnP) from microorganisms. This work investigated the transformation characteristics, the antibacterial activity of byproducts, and the degradation mechanism of tetracycline (TC) by purified MnP from Phanerochaete chrysosporium. The results show that nitrogen-limited and high level of Mn2+ medium could obtain favorable MnP activity and inhibit the expression of lignin peroxidase by Phanerochaete chrysosporium. The purified MnP could transform 80% tetracycline in 3 h, and the threshold of reaction activator (H2O2) was about 0.045 mmol L−1. After the 3rd cyclic run, the transformation rate was almost identical at the low initial concentration of TC (77.05–88.47%), while it decreased when the initial concentration was higher (49.36–60.00%). The antimicrobial potency of the TC transformation products by MnP decreased throughout reaction time. We identified seven possible degradation products and then proposed a potential TC transformation pathway, which included demethylation, oxidation of the dimethyl amino, decarbonylation, hydroxylation, and oxidative dehydrogenation. These findings provide a novel comprehension of the role of MnP on the fate of antibiotics in nature and may develop a potential technology for tetracycline removal.

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Ohmic Heating Technology for the Extraction of Chelating Soluble Pectin from Red Prickly Pear (Opuntia lasiacantha P.) Peel Biomass

Díaz-Cruz,

Contreras-Esquivel,

Benítez

et al. 2024

Food Bioprocess Technol

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Process performance improvement for the simultaneous production of ligninolytic enzymes in solid culture using agricultural wastes through the Taguchi method

Cited by 11 publications

References 36 publications

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Optimization of Endoglucanase Synthesis by Trichoderma harzianum via Taguchi Approach

Transformation of Tetracycline by Manganese Peroxidase from Phanerochaete chrysosporium

Ohmic Heating Technology for the Extraction of Chelating Soluble Pectin from Red Prickly Pear (Opuntia lasiacantha P.) Peel Biomass

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