Additive manufacturing (AM), particularly laser-based powder bed fusion of metals (LPBF), enables the fabrication of complex and customized metallic parts. However, 20–40% of the total manufacturing costs are usually attributed to post-processing steps. To reduce the costs of extensive post-processing, the process chain for AM parts has to be automated. Accordingly, robotic gripping and handling processes, as well as an efficient clamping for subtractive machining of AM parts, are key challenges. This study introduces and validates integrated bolts acting as a handling and clamping interface of AM parts. The bolts are integrated into the part design and manufactured in the same LPBF process. The bolts can be easily removed after the machining process using a wrench. This feasibility study investigates different bolt elements. The experiments and simulations conducted in the study show that a force of 250 N resulted in a maximum displacement of 12.5 µm. The milling results of the LPBF parts reveal a maximum roughness value, Ra, of 1.42 µm, which is comparable to that of a standard clamping system. After the bolt removal, a maximum residual height of 0.067 mm remains. Two case studies are conducted to analyze the form deviation, the effect of bolts on build time and material volume and to demonstrate the application of the bolts. Thus, the major contribution of this study is the design and the validation of standardized interfaces for robotic handling and clamping of complex AM parts. The novelties are a simple and clean interface removal, less material consumption, less support structure required and finally an achievement of a five-side tool accessibility by combining the interfaces with a three-jaw chuck.