Objective: to identify the factors impacting interpersonal relations in the nursing field. Method: cross-sectional, quantitative, descriptive, and exploratory study conducted with 213 nursing workers from a university hospital: 54 nurses, 69 nursing technicians, and 90 nursing aids. Data were collected from July to August 2016 using a semi-structured questionnaire addressing the participants’ characteristics and those of the work process, and a validated self-report questionnaire. Descriptive analysis, Pearson’s correlation coefficient, analysis of variance, and linear regression were performed, adopting a p-value<0.05. Results: affective constructions obtained the highest mean (4.62), showing this construct strengthen interpersonal relations at work, followed by administrative actions with a mean equal to 3.86, also a positive factor for interpersonal relations; however, workers seldom perceive their relational difficulties (1.72). The most significant factors were physical and emotional weariness accruing from interpersonal relations (p=0.000) and perception of emotional instability when facing relationship difficulties (p=0.010). Conclusion: interpersonal relations in the nursing field impact the workers’ health, with the potential to cause physical and emotional weariness though managerial actions and the workers’ behaviors also influence interpersonal relations. Thus, actions are needed to promote healthy interpersonal relations within institutions such as regular meetings and managerial support, fostering respect, cordiality, and empathy.