Processing and storage of blood components: strategies to improve patient safety

Efficient and highly reproducible production of red blood cell-derived extracellular vesicle mimetics for the loading and delivery of RNA molecules

“…Of note, prior to loading, RBCs samples have been isolated and purified by leukodepletion to completely remove granulocytes. The last ones are the major contaminants of blood derivatives, and their removal is in compliance with the guidelines for blood product processing [51][52][53] . The hypotonic loading method has been used for the entrapment of both dextran-conjugates and miRNAs in the mother cell (RBCs), according to the preloading strategy into mother cells for EV production, and showed a great cell recovery and loading efficiency.…”

Comparison of automated versus semi‐automated whole blood processing systems: A systematic review

“…Other beneficial aspects of automating blood processing systems include improved standardization of outputs, increased productivity, operator satisfaction and system usability, reduced wastage and reductions in required space and operator skill requirements, as well as reduced potential for repetitive strain injury to operators [3,5,6,9,32]. In addition, reduction in the required space and the number of operators also decreases the movement of operators around the workspace, which could play a major role in preventing the spread of diseases such as COVID-19.…”

“…Other authors have also reported that full automation may contribute to a balance between standardization and customization. Also, the released resources would enable the effortless implementation of other technologies in the blood component laboratory [3,5,32]. In addition, standardization of semi-automated processing methods remains highly variable among centres, with the remaining manual steps continuing to impact wastage as well as the quality and safety of blood products [3,5].…”

“…First, they are time consuming and labour intensive [2]. Second, any manual separation step must be carried out by highly skilled and qualified operators [2,3]. Third, regardless of the skill level and experience of the operator, the final quality of blood components prepared by manual methods is variable [4].…”

Moderne Methoden der Blutaufbereitung

“…Diese Me thode erfordert nur wenige technische Geräte und die Qualität der damit hergestellten Produkte hängt im Wesentlichen von der Ar beitsqualität der Mitarbeiter ab. Somit ist die manuelle Verarbei tung ein einfaches aber zeitaufwändiges und arbeitsintensives Ver fahren [17], welches die Expertise qualifizierter Fachkräfte erfor dert um die manuellen Separationsschritte präzise durchzuführen [18]. Des Weiteren variiert die Qualität manuell hergestellter Blut komponenten abhängig von der Erfahrung und Qualifikation des Personals [19].…”