Processing of a sporulation sigma factor in Bacillus subtilis: How morphological structure could control gene expression

Stragier, Patrick; Bonamy, Celine; Karmazyn-Campelli, Céline

doi:10.1016/0092-8674(88)90407-2

Cited by 394 publications

(413 citation statements)

References 25 publications

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“…In addition to confirming that SpoIIGA is the pro-~E processing enzyme as suggested originally (Stragier et al 1988), these experiments indicate that no other csf gene is involved in the pathway, as demonstrated further by the bypass of crv for pro-r E processing observed when csfX is expressed through an early sporulation promoter independent of (yr. The PspolIG-csfX construct has no negative effect on sporulation of a wild-type strain and Figure 6.…”

Section: The Role Of Csfxmentioning

confidence: 58%

“…This fusion was cloned in the pDG268 vector (Antoniewski et al 1990) and introduced into the chromosome at the amyE locus (Shimotsu and Henner 1986). The spolID-lacZ transcriptional fusion integrated at the amyE locus has been described previously as well as the conditions of culture and 13-galactosidase assay (Stragier et al 1988). All of the strains used are derivatives of strain JH642.…”

Section: Lacz Fusionsmentioning

confidence: 99%

“…The Pspac-csfX construct fully complements the sporulation defect of a csfX mutant when grown in the presence of 1 mM IPTG. The multicopy plasmids carrying various parts of the spollG operon (encoding SpolIGA and pro-e E) under the control of the spac promoter have been described previously (Stragier et al 1988). The BfaIXhoI fragment carrying the csfX-coding sequence was also cloned downstream of the spoHG promoter [which is activated shortly after the onset of sporulation (Kenney and Moran 1987)],…”

Section: Heterochronic Expression Of Csfxmentioning

confidence: 99%

“…r is synthesized in the predivisional cell (Gholamhoseinian and Piggot 1989) as an inactive larger precursor, pro-tx E (LaBell et al 1987) the product of the spoHGB gene (Stragier et al 1984;Trempy et al 1985a), which is cotranscribed with spolIGA, the gene encoding the putative pro-or E processing enzyme (Stragier et al 1988). SpoIIGA is predicted to be a membrane-bound protein (Stragier et al 1988;Peters and Haldenwang 1991 ) and is also synthesized before septation, suggesting that it is present on both sides of the septum. It has been hypothesized that cr F is involved in generating the signal that triggers pro-or E processing, by inducing transcription of a gene, X, whose product acts vectorially from the forespore to the mother cell (Losick and Stragier 1992).…”

mentioning

confidence: 99%

“…It is initiated by activation of the r factor in the smaller cell, the forespore (Margolis et al 1991;Partridge et al 1991), which is required for subsequent activation of the cE factor in the larger cell, the mother cell Losick and Stragier 1992). r is synthesized in the predivisional cell (Gholamhoseinian and Piggot 1989) as an inactive larger precursor, pro-tx E (LaBell et al 1987) the product of the spoHGB gene (Stragier et al 1984;Trempy et al 1985a), which is cotranscribed with spolIGA, the gene encoding the putative pro-or E processing enzyme (Stragier et al 1988). SpoIIGA is predicted to be a membrane-bound protein (Stragier et al 1988;Peters and Haldenwang 1991 ) and is also synthesized before septation, suggesting that it is present on both sides of the septum.…”

mentioning

confidence: 99%

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Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis.

Londoño-Vallejo

Stragier²

1995

Genes Dev.

123

142

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Transcription in the mother cell at early stages of sporulation in Bacillus subtilis is controlled by orE, a or factor that is synthesized in the predivisional cell as an inactive larger precursor, pro-or E. Activation of ore depends on orF, the factor that governs transcription in the forespore. Genetic experiments have indicated that transduction of the activation signal from the forespore to the mother cell requires the products of some genes belonging to the orF-controlled regulon. We have identified and characterized a orF-dependent gene, csfX, encoding a protein necessary and sufficient for triggering processing of pro-or E. The CsfX protein contains a typical amino-terminal signal sequence suggesting that, although synthesized in the forespore, it may act across the septum to activate the membrane-bound enzyme that is responsible for pro-or E processing in the mother cell.

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Section: The Role Of Csfxmentioning

confidence: 58%

Section: Lacz Fusionsmentioning

confidence: 99%

Section: Heterochronic Expression Of Csfxmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis.

Londoño-Vallejo

Stragier²

1995

Genes Dev.

123

142

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What the papers say: Compartmentalized transcription and the establishment of cell type during sporulation in Bacillus subtilis

Gober

1992

BioEssays

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An early step in sporulation of the bacterium Bacillus subtilis, is the formation of two compartments in the developing sporangium: the mother cell and the forespore. These compartments differ in their programs of gene expression and developmental fate. The establishment of cell type within this simple developmental program, is accomplished by the compartmentalization of sigma subunits of RNA polymerase. The localization of these sigma factors results in compartment-specific gene expression. Recent experiments have elucidated some of the early steps in the establishment of cell type. After septum formation, the activity of the sigma factor, sigma F, is confined to the forespore compartment. This, in turn, results in the localized expression of another developmental sigma factor, sigma G. The forespore localization of these two sigma factors, establishes the forespore line of gene expression. sigma F and sigma G also regulate mother cell events. sigma F activity in the forespore regulates the proteolytic processing of sigma E within the mother cell compartment. The localization sigma E activity leads to mother cell expression of another sigma factor, pro-sigma K. The proteolytic processing of pro-sigma K to mature sigma K is controlled by the forespore sigma factor, sigma G. Mature sigma K then directs the transcription of mother cell specific genes. Therefore, the initial localization of sigma F activity to the forespore compartment, orchestrates the establishment of cell type in both forespore and mother cell compartments.

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Studies of transcriptional state heterogeneity in sporulating cultures of bacillus subtilis

Chung

Stephanopoulos

1995

Biotech & Bioengineering

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Using a flow cytometry-based technique for detecting p-galactosidase. lacZ fusions have been used to study the pattern of gene expression exhibited by stationary phase cultures of Bacillus subtilis that have initiated the developmental pathway of spore formation. We have found that within such cultures there exist two distinct cell types: one that has induced the developmental program of gene expression and one that has not. This heterogeneity among transcriptional states is shown to be established early during the stationary phase and plays a significant role in influencing later stationary phase events. Additionally, when this technique is used to study gene expression in mutants that display altered patterns of gene expression, we are able to conclude that the cellular apparatus responsible for sensing and transducing stationary phase developmental signals represents a bottleneck that controls the expression of certain early stationary phase genes. These findings provide a demonstration of the utility of single cell measurements of gene expression and indicate that unless culture heterogeneity is properly taken into account, standard measurements of gene expression may not provide information suitable for the analysis of events occurring at the cellular level. (c) 1995 John Wiley & Sons, Inc.

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Processing of a sporulation sigma factor in Bacillus subtilis: How morphological structure could control gene expression

Cited by 394 publications

References 25 publications

Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis.

Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis.

What the papers say: Compartmentalized transcription and the establishment of cell type during sporulation in Bacillus subtilis

Studies of transcriptional state heterogeneity in sporulating cultures of bacillus subtilis

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