Processing of Lipoaspirate Samples for Optimal Mesenchymal Stem Cells Isolation

Soft tissue fillers as non‐specific modulators of adipogenesis: change of the paradigm?

“…Possible mechanism can involve the diversion of ADSC to pathological adipocytes in hyperglycaemia . Inflammation in sWAT, being the hallmark of this tissue in obese patients, can even change the phenotype of ADSC, thus providing the reason to distinguish between ‘healthy’ and ‘non‐healthy’ ADSC . This can mean that the long‐term results of STFs can be dependent even on the metabolic history of the local ADSC.…”

Enhancement of Human Adipose-Derived Stem Cell Expansion and Stability for Clinical use

“…Recent techniques have shown that less aggressive enzymatic treatment using trypsin could be employed for better cellular stability but had lower overall cell yields. Reports have demonstrated that although mechanically isolated ASCs (mincing) revealed lower cell numbers, the viability was higher [15][16][17]. In addition, Zeng et al [18] have shown isolation of ASCs without collagenase or trypsin although they treated the tissue for 3 consecutive days with 100% fetal bovine (FBS) serum to "digest" the tissue and allow cells to emerge from the tissue mass.…”

A Simple Apparatus for Testing and Washing Filter Candles

“…blood, debris, anesthetics, and other substances that are used in liposuction, and free lipid that is released from disrupted adipocytes. Therefore, processing lipoaspirate may include extensively washing the adipose tissue fragments to separate them from other materials [2][3][4][5][6][7][8], followed by dissociation of the cells either enzymatically [2][3][4][5][6] or mechanically [7], so that they are ready to be cultured. All of these processes may be conducted in a closed system device [8].…”

“…There are various methods to wash the adipose tissue fragments. In most methods, an equal volume of washing solution is added, mixed, and followed by either centrifugation [2,3] or decantation after 3 to 30 minutes of sedimentation in bottles [4,5], using a separation funnel [6] or a syringe [7]. Finally, the floating adipose tissue fragments are collected either directly [2,3] or by removing the infranatant using a pipette [4], suction [5], or ejection from the separation funnel [6] or syringe [7].…”

“…In addition to providing adipose-derived stem cell culture, lipoaspirate washing is required in fat grafting. However, lipoaspirate washing in fat grafting should be gentle to avoid rupture of the mature adipocytes because the washed adipose tissue fragments will be reinserted into the patient, and therefore, the centrifugation method should be avoided [7,9]. For fat grafting purposes, washing of adipose tissue fragments is preferably done by decantation [7,9], or filtering using Telfa gauze (Kendall, Chicago, IL, USA) that is an ideal washing technique for fat graft survival [10,11].…”