Processing of RNA in Escherichia coli is limited in the absence of ribonuclease III, ribonuclease E and ribonuclease P

Plautz, Greg; Apirion, David

doi:10.1016/0022-2836(81)90360-0

Cited by 15 publications

(5 citation statements)

References 13 publications

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“…The DNA sequence of the lOSb RNA gene (12) suggests that there is a promoter (Pribnow box) that ends six nucleotides upstream from 10Sb RNA. The reason for the failure to identify a 5' triphosphate is unclear because 5' triphosphate nucleosides were previously identified in different size molecules isolated from RNA processing mutants (36,37).…”

Section: Discussionmentioning

confidence: 99%

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Gurevitz¹,

Jain²,

Apirion³

1983

Proc. Natl. Acad. Sci. U.S.A.

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A precursor molecule for lOSb (MI) RNA, the RNA moiety of the RNA processing enzyme ribonuclease P (EC 3.1.26.5), is accumulated transiently in an Escherichia coli strain containing a plasmid that carries the lOSb RNA gene. The same RNA precursor molecule is accumulated, in relatively large quantities, in a temperature-sensitive RNase E-mutant at the nonpermissive temperature. The RNA precursor includes lOSb RNA and an extra 3' fragment that contains a termination stem and loop. It can be processed in vitro to a molecule the size of lOSb RNA. None of the four endoribonucleases of E. coli-RNase III, RNase E, RNase F, or RNase P-takes part in this cleavage reaction. Therefore, we suggest that the processing of the precursor-IOSb RNA to iOSb RNA is carried out by a thus-far unidentified endoribonuclease. The accumulation of a RNA molecule in a RNase E-mutant that does not contain a cleavage site for RNase E has been encountered previously and can be explained by assuming the existence of a RNA processing complex in the E. coli cell.Processing of RNA is an important aspect of RNA metabolism that plays a key role in the determination of the final population of RNA molecules in the cell. RNA processing involves a set of reactions that happen post-transcriptionally and prepares a RNA molecule for its function. The involvement of the three endonucleolytic RNA processing enzymes RNase III, RNase E, and RNase P has been well documented in the processing of Escherichia coli rRNA and tRNA (1-6).The processing enzyme RNase P is unique among all RNA processing enzymes because it consists of a protein and a RNA moiety (7-12). The RNA, MI, is required for RNase P activity (9) and is identical to the 10Sb RNA that accumulates in a strain containing the plasmid pL2 (pLN2) that partially complements the rnpA49 mutation (13,14). Introduction of the pL2 plasmid to E. coli strains leads to the accumulation of 1OSb (Ml) RNA, which is not a primary transcript (12)(13)(14).In the studies reported here we show that 10Sb (Ml) RNA, the RNA of RNase P, is derived from a precursor molecule (plOSb) that contains extra sequences at the 3' end, and we have established conditions for the in vitro processing of this precursor RNA. These experiments helped to identify a previously unrecognized RNA processing activity in E. coli. This precursor accumulates in a strain defective in the RNA processing enzyme RNase E, even though the enzyme RNase E does not process this RNA molecule. Therefore, the finding that this precursor accumulates in a RNase E-strain strengthens the concept of a complex of RNA processing enzymes in the E. coli cell.MATERIALS AND METHODS Strains. The rnp+ strain used was PP113 (15) and the rnpA49 strain was N2020 (16). The rne strain was N3441, which was derived from strain N3431 (17). To these strains the plasmid pL2 was introduced. This plasmid contains E. coli DNA that carries the gene for lOSb RNA (13). N2069 is a RNase III-strain derived from strain N7060 (18).Growth and Labeling of Cells. Cells were grown in lowph...

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Section: Discussionmentioning

confidence: 99%

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Gurevitz¹,

Jain²,

Apirion³

1983

Proc. Natl. Acad. Sci. U.S.A.

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“…However, studies using mutants that are compromised in RNase III activity demonstrated that this enzyme only has a minor role in processing (Gegenheimer and Apirion 1981). In fact, early tRNA maturation only becomes severely compromised if this mutation is introduced into a strain that is also deficient in RNase P and RNase E activities, suggesting the latter two proteins have the primary roles in this process (Plautz and Apirion 1981).…”

Section: Processing Of Trna Precursorsmentioning

confidence: 99%

Bacterial transfer RNAs

Shepherd¹,

Ibba²

2015

FEMS Microbiology Reviews

109

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Transfer RNA is an essential adapter molecule that is found across all three domains of life. The primary role of transfer RNA resides in its critical involvement in the accurate translation of messenger RNA codons during protein synthesis and, therefore, ultimately in the determination of cellular gene expression. This review aims to bring together the results of intensive investigations into the synthesis, maturation, modification, aminoacylation, editing and recycling of bacterial transfer RNAs. Codon recognition at the ribosome as well as the ever-increasing number of alternative roles for transfer RNA outside of translation will be discussed in the specific context of bacterial cells.

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“…Another point of interest is that in a triplc mc', m e , r p mutant at the elevated temperature most of the accumulated small RNAs contain a 5' triphosphate (21), which indicates that they are unprocessed RNAs. This finding suggests that these three endonucleolytic RNA processing enzymes, RNase IJI, E and P constitute most of the enzymatic endonucleolytic RNA processing machinery in the E. coli cell.…”

Section: Trna Processing In Escherichia Colimentioning

confidence: 99%

RNA processing in prokaryotic cells

Apirion

Miczák

1993

BioEssays

Self Cite

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RNA processing in Escherichia coli and some of its phages is reviewed here, with primary emphasis on rRNA and tRNA processing. Three enzymes, RNase III, RNase E and RNase P are responsible for most of the primary endonucleolytic RNA processing events. The first two are proteins, while RNase P is a ribozyme. These three enzymes have unique functions and in their absence, the cleavage events they catalyze are not performed. On the other hand a relatively large number of exonucleases participate in the trimming of the 3' ends of tRNA precursor molecules and they can substitute for each other. Primary processing is the first event that happens to the nascent RNA molecule, while in secondary RNA processing, the substrate is a product of a primary processing event. Although most RNA processing occurs in RNP particles, it seems that only in secondary RNA processing is the RNP particle required for the reaction. Bacteria and especially bacteriophages contain self-splicing introns which in cases were probably acquired from other species.

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Processing of RNA in Escherichia coli is limited in the absence of ribonuclease III, ribonuclease E and ribonuclease P

Cited by 15 publications

References 13 publications

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Identification of a precursor molecular for the RNA moiety of the processing enzyme RNase P.

Bacterial transfer RNAs

RNA processing in prokaryotic cells

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