Processing of semen can result in increased sperm DNA fragmentation

Toro, E. Macías del; Fernández, Silvia; Colomar, A; Casanovas, A; Álvarez, Juan G.; López-Teijón, M; Velilla, Esther

doi:10.1016/j.fertnstert.2009.05.059

Cited by 33 publications

(17 citation statements)

References 19 publications

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“…Because the Cryotop is considered to be a suitable carrier for the reason of a higher recovery rate, which does not mean that the Cryotop method can reduce the DNA fragmentation. Another, possible factor is the incubation times previously to determiner this parameter (Toro et al, 2009). However, the increment of DNA fragmentation may produce an adverse effect on ICSI outcome, which will hamper its clinical application.…”

Section: Discussionmentioning

confidence: 99%

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Chen

Qian

et al. 2014

Andrologia

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Cryotop is a carrier that has been used successfully in the cryopreservation of human spermatozoa. Here, we explored a novel method to vitrify human spermatozoa without cryoprotective agents (CPAs) using Cryotop. Spermatozoa from 21 Normozoospermic patients were collected and vitrified without CPAs or with sucrose in small volume using Cryotop. The sperm recovery rate, motility, viability, chromatin damage and DNA fragmentation were assessed. No significant difference was observed in the sperm recovery rate and motility rate between the spermatozoa cryopreserved without CPAs and with sucrose. The post-thawed spermatozoa cryopreserved without CPAs had a higher viability and lower damage to sperm chromatin and DNA than those cryopreserved with sucrose. These results suggest that small numbers of human spermatozoa can be successfully vitrified without CPAs using Cryotop.

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Section: Discussionmentioning

confidence: 99%

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Chen

Qian

et al. 2014

Andrologia

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“…The mere manipulation of sperm in the laboratory introduces DNA fragmentation (Toro et al 2009(Toro et al , 2110, as does cryopreservation (Zribi et al 2010, 162), and the thawing process (Gosalvez et al 2009, 171-172). This sperm DNA fragmentation results in poorer embryo quality, as well as poorer fertilization and pregnancy rates (Simon et al 2011, 724).…”

Section: Vi) Physical Harmsmentioning

confidence: 99%

Conceptualising a Child-Centric Paradigm

Adams

2013

Bioethical Inquiry

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Since its inception, donor conception practices have been a reproductive choice for the infertile. Past and current practices have the potential to cause significant and lifelong harm to the offspring through loss of kinship, heritage, identity, family health history and possibly introducing physical problems. Legislation and regulation in Australia that specifies that the welfare of the child born as a consequence of donor conception is paramount may therefore be in conflict with the outcomes. Altering the paradigm to a child centric model however, impinges on reproductive choice and rights of adults involved in the process. With some lobby groups pushing for increased reproductive choice while others emphasise offspring rights there is a dichotomy of interests that society and legislators need to address. Concepts pertaining to a shift towards a child centric paradigm are discussed.

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“…There are numerous studies demonstrating that SDF and apoptotic markers increase after cryopreservation, mostly because of an increase in oxidative stress. 31,[52][53][54] Thus, antioxidants could be added to cryopreservation media, as suggested by studies showing some efficacy of genistein and resveratrol in reducing postthaw SDF. 53,54 Finally, several studies have evaluated the relationship between varicocele and SDF, and the effect of varicocele surgical or microsurgical repair on SDF.…”

Section: Clinical Correlatesmentioning

confidence: 99%

“…31,[52][53][54] Thus, antioxidants could be added to cryopreservation media, as suggested by studies showing some efficacy of genistein and resveratrol in reducing postthaw SDF. 53,54 Finally, several studies have evaluated the relationship between varicocele and SDF, and the effect of varicocele surgical or microsurgical repair on SDF. Men with varicoceles exhibit higher SDF levels than fertile men or donors, but these levels are not higher than those of men with other or unknown (idiopathic) causes of SDF.…”

Section: Clinical Correlatesmentioning

confidence: 99%

Mechanisms and clinical correlates of sperm DNA damage

Tamburrino¹,

Marchiani²,

Montoya³

et al. 2011

Asian J Androl

117

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Among the different DNA anomalies that can be present in the male gamete, DNA fragmentation is the most frequent, particularly in infertile subjects. There is now consistent evidence that a sperm containing fragmented DNA can be alive, motile, morphologically normal and able to fertilize an oocyte. There is also evidence that the oocyte is able to repair DNA damage; however, the extent of this repair depends on the type of DNA damage present in the sperm, as well as on the quality of the oocyte. Thus, it is important to understand the possible consequences of sperm DNA fragmentation (SDF) for embryo development, implantation, pregnancy outcome and the health of progeny conceived, both naturally and by assisted reproductive technology (ART). At present, data on the consequences of SDF for reproduction are scarce and, in many ways, inconsistent. The differences in study conclusions might result from the different methods used to detect SDF, the study design and the inclusion criteria. Consequently, it is difficult to decide whether SDF testing should be carried out in fertility assessment and ART. It is clear that there is an urgent need for the standardisation of the methods and for additional clinical studies on the impact of SDF on ART outcomes.

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Processing of semen can result in increased sperm DNA fragmentation

Cited by 33 publications

References 19 publications

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop

Conceptualising a Child-Centric Paradigm

Mechanisms and clinical correlates of sperm DNA damage

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