Background, aim, and scope Facing the threat of oil depletion and climate change, a shift from fossil resources to renewables is ongoing to secure long-term low carbon energy supplies. In view of the carbon dioxide reduction targets agreed upon in the Kyoto protocol, bioethanol has become an attractive option for one energy application, as transport fuel. Many studies on the LCA of fuel ethanol have been conducted, and the results vary to a large extent. In most of these studies, only one type of allocation is applied. However, the effect of allocation on outcomes is of crucial importance to LCA as a decision supporting tool. This is only addressed in a few studies to a limited extent. Moreover, most of the studies mainly focus on fossil energy use and GHG emissions. In this paper, a case study is presented wherein a more complete set of impact categories is used. Land use has been left out of account as only hectare data would be given which is obviously dominated by agriculture. Moreover, different allocation methods are applied to assess the sensitivity of the outcomes for allocation choices. Materials and methods This study focuses on the comparison of LCA results from the application of different allocation methods by presenting an LCA of gasoline and ethanol as fuels and with two types of blends of gasoline with ethanol, all used in a midsize car. As a main secondgeneration application growing fast in the USA, corn stover-based ethanol is chosen as a case study. The life cycles of the fuels include gasoline production, corn and stover agriculture, cellulosic ethanol production, blending ethanol with gasoline to produce E10 (10% of ethanol) and E85 (85% of ethanol), and finally the use of gasoline, E10, E85, and ethanol. In this study, a substantially broader set of eight environmental impacts is covered. Results LCA results appear to be largely dependent on the allocation methods rendered. The level of abiotic depletion and ozone layer depletion decrease when replacing gasoline by ethanol fuels, irrespective of the allocation method applied, while the rest of the impacts except global warming potential are larger. The results show a reduction of global warming potential when mass/energy allocation is applied; in the case of economic allocation, it gives contrary results. In the expanded systems, global warming potential is significantly reduced comparing to the ones from the allocated systems. A contribution analysis shows that car driving, electricity use for cellulase enzyme production, and ethanol conversion contribute largely to global warming potential from the life cycle of ethanol fuels. Discussion The reason why the results of global warming potential show a reverse trend is that the corn/stover allocation ratio shifts from 7.5 to 1.7 when shifting from economic allocation to mass/energy allocation. When mass/energy allocation is applied, both more credits (CO 2 uptake) and more penalties (N 2 O emission) in agriculture are allocated to stover compared to the case of economic allocation. However, mo...