Additive Manufacture (AM) of Ti-6Al-4V generally leads to an undesirable microstructure with a non-random texture. The alpha texture is inherited from large columnar grains that grow across the deposited layers with a strong preferential <001> growth direction. It has been found in AM that the application of a surprisingly small amount of plastic strain to each layer, by methods such as in-process rolling, can disrupt the columnar growth and produce a more randomly orientated, fine equiaxed grain structure, and consequently a refined final microstructure and far weaker alpha texture. The origin of this interesting effect was investigated by direct in-situ observation of the formation of new grain orientations, within the retained deformed beta, and their growth on reheating near to the transus temperature, by EBSD analysis. This analysis has shown that colonies twin during deformation, which generates new beta orientations during reheating.