Processing Parameters Matching Effects upon Rhizobium tropici Biopolymers’ Rheological Properties

Abstract

The combined effects of the processing parameters upon rheological properties of biopolymers produced by Rhizobium tropici were studied as a function of the Ca(+2) ions' concentration variation, yeast extract concentration added to the medium, aeration, and agitation, maintaining the mannitol concentration in 10 g/L. The experiments were carried out using a fermenter with 20-L capacity as a reactor. All processing parameters were monitored online. The temperature [(30 +/- 1) degrees C] and pH values (7.0) were kept constant throughout the experimental time. As a statistical tool, a complete 2(3) factorial design with central point and response surface was used to investigate the interactions between relevant variables of the fermentation process: calcium carbonate concentration, yeast extract concentration, aeration, and agitation. The processing parameter setup for reaching the maximum response for rheological propriety production was obtained when applying mannitol concentration of 10.0 g/L, calcium carbonate concentration 1.0 g/L, yeast extract concentration 1.0 g/L, aeration 1.30 vvm, and agitation 800 rpm. The viscosimetric investigation of polysaccharide solutions exposed their shear-thinning behavior and polyelectrolytic feature.

Poly-β-hydroxybutyrate and Exopolysaccharide Biosynthesis by Bacterial Isolates from Pigeonpea [Cajanus cajan (L.) Millsp] Root Nodules

“…EPS was precipitated with the addition of three volumes of cold ethanol (95%) to the supernatant of the first centrifugation as described before [11,16,17]. The extract was centrifuged again at 10,000×g for 30 min.…”