Processing ThinPrep cervical cytological samples for Raman spectroscopic analysis

Bonnier, Franck; Traynor, Damien; Kearney, Padraig; Clarke, Colin; Knief, Peter; Martin, Cara; O’Leary, John; Byrne, Hugh J.; Lyng, Fiona M.

doi:10.1039/c4ay01497a

Cited by 38 publications

(68 citation statements)

References 58 publications

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“…A recent study from our group [35] addressed many of the issues involved in recording Raman spectra from ThinPrep cervical cytology samples. A new method based on pre-treatment of the slides with hydrogen peroxide to clear blood residue contamination before Raman recording was shown to minimise variability within the data sets.…”

Section: Raman Spectroscopy For Cytopathologymentioning

confidence: 99%

“…Table 2 costs should be favourable when compared to the imaging systems or to manual scoring where personnel costs could be reduced. A recent study by our group [35] has shown that glass ThinPrep slides can be used for Raman spectral recording in place of spectroscopic substrates, such as calcium fluoride substrates. These substrates reduce the presence of confounding contributions of the substrate but they are considerably more expensive than the glass slides used in the cytopathology laboratory.…”

Section: Future Perspectivesmentioning

confidence: 99%

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Raman spectroscopy for screening and diagnosis of cervical cancer

et al. 2015

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Section: Raman Spectroscopy For Cytopathologymentioning

confidence: 99%

Section: Future Perspectivesmentioning

confidence: 99%

Raman spectroscopy for screening and diagnosis of cervical cancer

et al. 2015

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“…The glass substrate contributes its own spectrum, which can significantly obscure the cellular spectra. However, using the 532 nm wavelength as a source and a Raman microscope equipped with a 100x objective, the contribution from the substrate is considerably reduced [17,19]. Furthermore, any residual contribution from the glass in the finger print region (400-1800 cm -1 ) has been removed by using a nonnegatively constrained least squares (NNLS) method.…”

Section: Data Preprocessing and Analysismentioning

confidence: 99%

“…The potential of vibrational spectroscopy for clinical diagnosis based on exfoliated epithelial cells has already been demonstrated for the case of cervical cancer [8][9][10]. A limited number of infrared absorption spectroscopy studies have demonstrated the feasibility of using oral exfoliated cells.…”

Section: Introductionmentioning

confidence: 99%

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Development of methodology for Raman microspectroscopic analysis of oral exfoliated cells

Behl¹,

Calado²,

Ibrahim³

et al. 2017

Anal. Methods

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Oral squamous cell carcinoma ranks as the 15th most common cancer worldwide. The present study was undertaken to standardise a protocol for the analysis of oral exfoliated cells using Raman microspectroscopy. For this purpose, samples were obtained from two different sites, based on prevalence of disease (ventral side of the tongue and buccal mucosa).Different oral rinsing agents were employed and it was concluded that non-alcoholic mouthwash adequately removes food debris. Samples were collected using various collection tools and compared. It was observed that endo-cervical brushes yielded cells from deeper layers of the epithelium. Furthermore, monolayer formation of cells was carried out adopting cytospin and ThinPrep techniques and only the ThinPrep method provided flat and separated cells on the glass slide. Raman spectra were acquired from the nuclear and cytoplasmic regions of the cell using an XploRA confocal Raman instrument (HORIBA Jobin Yvon) with a 532nm laser as source. Glass spectral contamination was removed using non negatively constrained least squares (NNLS) algorithms. Corrected spectra were subjected to principal components analysis (PCA) which was able to differentiate the nucleus and cytoplasm regions of the cell; based on nucleic acid and protein features respectively. However, no classification of the two anatomically different sites was observed according to PCA or PCA-LDA (Linear discriminant analysis) using either the nuclear or cytoplasmic spectra.Nevertheless, the study has developed a standardised protocol for sample collection, sample preparation, spectral acquisition and data processing for future studies of oral exfoliated cells based on Raman microspectroscopy.

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A study of hormonal effects in cervical smear samples using Raman spectroscopy

Traynor

Kearney

Ramos

et al. 2018

Journal of Biophotonics

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Raman spectroscopy is a powerful tool that has the potential to be used as a screening method for cervical cancer. It is a label-free, low-cost method providing a biochemical fingerprint of a given sample. The objective of this study was to address patient-to-patient variability contributed by hormonal effects due to the menstrual cycle, the use of hormone-based contraceptives (HC) and the onset of menopause, and to determine if these changes would affect the ability to successfully identify dyskaryotic cells. Raman spectra were recorded from unstained ThinPrep cervical samples (45 cytology negative and 15 high-grade dyskaryosis (high-grade squamous intraepithelial lesion, HSIL) samples using a HORIBA Jobin Yvon XploRA system. HPV DNA testing was also performed. Clinical data collected included date of the last menstrual period, the use of HC and/or menopausal status. Spectral changes were observed depending on the day of the menstrual cycle and on the use of HC. Despite this, HSIL could be discriminated from normal cells regardless of the day on which the sample was taken or the use of HC.

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Processing ThinPrep cervical cytological samples for Raman spectroscopic analysis

Cited by 38 publications

References 58 publications

Raman spectroscopy for screening and diagnosis of cervical cancer

Raman spectroscopy for screening and diagnosis of cervical cancer

Development of methodology for Raman microspectroscopic analysis of oral exfoliated cells

A study of hormonal effects in cervical smear samples using Raman spectroscopy

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