Prochloraz and coumaphos induce different gene expression patterns in three developmental stages of the Carniolan honey bee (Apis mellifera carnica Pollmann)

Cizelj, Ivanka; Glavan, Gordana; Božič, Janko; Oven, Irena; Mrak, Vesna; Narat, Mojca

doi:10.1016/j.pestbp.2015.09.015

Cited by 51 publications

(24 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1-fold and 0.5-fold, respectively, p<0.0002), which corroborates the findings of Yang and Cox-Foster (2005). Decreased lys-2 gene expression was also noted in the prepupae and imago of Carniolan honey bees exposed to pesticides (Cizelj et al, 2016). The above effects may be strengthened, making V. destructor-infested bee colonies more susceptible to bacterial and viral infections.…”

Section: Resultssupporting

confidence: 85%

Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor

Zaobidna

Zoltowska

Łopieńska–Biernat

2017

Journal of Apicultural Science

View full text Add to dashboard Cite

A b s t r a c tVarroa destructor is a parasitic mite that attacks the honey bee, and previous studies have suggested that parasitosis caused by this mite is accompanied by immunosuppresion in the host. In this study, the effect of mite infestation on the expression of the lysozyme-1 (lys-1) gene and lysozyme activity in Apis mellifera carnica was determined. The experiment was carried out on the five developmental stages of honey bee workers and drones. Developmental and gender-related differences in gene expression and lysozyme activity were observed in a Varroa destructor-infested brood. The relative expression of the lys-1 gene increased in a infested worker brood and decreased in a drone brood except for P3 pupae. In the final stage of development, the lys-1 gene expression was significantly lower in infested newly emerged workers and drones. Changes in the relative expression of the lys-1 gene in infested individuals was poorly manifested at the level of enzyme activity, whereas at the two final stages of development (P5 and I) there was a positive correlation between relative lys-1 expression and lysozyme activity in infested bees of both genders (r=0.988, r=0.999, respectively). The results of this study indicate that V. destructor influences the lysozyme-linked immune response in bees.

show abstract

Section: Resultssupporting

confidence: 85%

Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor

Zaobidna

Zoltowska

Łopieńska–Biernat

2017

Journal of Apicultural Science

View full text Add to dashboard Cite

show abstract

“…It was previously found to increase almost 1,000-fold the toxicity of tau-fl uvalinate, and more than 20-fold that of coumaphos and fenpyroximate [149]. Moreover, prochloraz altered the immune-gene expression in honey bees used alone and in combination with coumaphos [63,151]. Recently, Glavinic et al [138] monitored the expression of 15 immune-related genes in adult honey bees, and found that it may be affected when food contaminated with prochloraz was consumed by bee larvae (Figure 9).…”

Section: Infestation With Varroa Destructor and Mixed Virus Infectionsmentioning

confidence: 97%

Looking for the causes of and solutions to the issue of honey bee colony losses

et al. 2019

View full text Add to dashboard Cite

Colony losses, including those induced by the colony collapse disorder, are an urgent problem of contemporary apiculture which has been capturing the attention of both apiculturists and the research community. CCD is characterized by the absence of adult dead bees in the hive in which few workers and a queen remain, the ratio between the brood quantity and the number of workers is heavily disturbed in favor of the former, and more than enough food is present. Robbing behavior and pests usually attacking the weakened colony do not occur. In the present paper, the causes of the emergence of this problem are discussed, as well as the measures of its prevention. The following factors, which lead to colony losses, are analyzed: shortage of high-quality food (pollen and honey); infestation with parasites, primarily with Varroa destructor, and mixed virus infections; bacterial infections (American and European foulbrood), fungal infections (nosemosis and ascosphaerosis) and trypanosomal infections (lotmariosis); and, finally, general management of the apiary. Certain preventive measures are proposed: (1) providing ample high-quality forage and clean water, (2) avoiding sugarisation, i.e. superfluous use of sugar syrup, (3) meeting the nutritional needs of the colony, (4) when feeding bees, taking care of the timing and the composition of diet, avoiding pure sugar syrup which in excessive quantities may induce energetic and oxidative stress, (5) when there is a shortage of natural feed – honey in the brood chamber – use sugar syrup with natural/artificial supplements to avoid protein starvation, (6) organized control of V. destructor in the colonies is obligatory due to its vector role, and (7) compliance with hygienic and sanitary measures and principles of good apiculture practice and management in apiaries. To conclude, all preventive measures are feasible in compliance with rules and regulations concerning regular spring and autumn bee health monitoring by licensed veterinarians, who can propose adequate treatments if necessary.

show abstract

“…Coumaphos and tau-fluvalinate have been found to impair olfactory learning (Taylor et al 1987, Williamson and. Coumaphos has additionally been found to downregulate immune genes in prepupal bees (Cizelj et al 2016). Teeters et al (2012) found that tau-fluvalinate decreased locomotor activity in bees.…”

Section: Varroa Destructor (Anderson and Trueman) Mite (Mullin Et Almentioning

confidence: 99%

“…Coumaphos and tau-fluvalinate, the active ingredients of the miticides Checkmite+ ® and Apistan ® , are the two most commonly found pesticides within managed bee colonies , Garcia et al 2017). Exposure to these miticides can have negative effects associated with olfactory learning, immune gene suppression, and decreased locomotor activity in bees , Teeters et al 2012, Cizelj et al 2016. Several pesticides in the neonicotinoid class negatively affect immunocompetence, gland and respiratory development in bees, as well as reducing the survivability of queens exposed to other stressors (Hatjina et al 2013, Chaimanee et al 2016, Dussaubat et al 2016.…”

Section: Introductionmentioning

confidence: 99%

“…The two most common pesticides found in hives are coumaphos and tau-fluvalinate, the miticides used in Checkmite+ ® and Apistan ® , respectively. These acaricides have been shown to affect olfactory learning, downregulate immune genes, and decrease locomotor activity in bees , Teeters et al 2012, Cizelj et al 2016, Garcia et al 2017. A recent study found that chronic exposures to the neonicotinoid thiacloprid affected bee navigation, motivation to forage, and social communication .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chemical manipulation of honey bee behavior

Larson¹

2016

2016 International Congress of Entomology

View full text Add to dashboard Cite

The loss of managed honey bee colonies, resulting from their unintentional exposure to pesticides, is a topic of concern for the agricultural and apicultural industry. Current methods for reducing pesticide exposure to bees involve the application of pesticides before crop bloom or in the evening when foraging bees are less likely to be exposed to these applications. There is an urgent need for additional protection procedures to reduce the annual losses of managed bee colonies. Another method for protecting these pollinators is the use of chemical deterrents to reduce the interaction times of foraging bees with pesticide-treated crops. Historically, insect repellents (IRs) have been used to prevent the spread of deadly human diseases by arthropod vectors. However, it has been shown that bees can be repelled from pesticide-treated crops using DEET and bee pheromonal compounds. Here, I report the toxicological and deterrent effects of bee pheromonal compounds, as well as the deterrent effects of heterocyclic amines (HCAs) on bees. The results of this study indicate that the bee pheromonal compounds, at 8, 20, 60 and 100% concentrations, are toxic to bees and inhibit the feeding of bees within a confined space. Additionally, the pheromonal compounds and the HCAs are as efficacious as DEET in deterring bees from treated food sources.The HCA piperidine, was observed to effectively deter bee foragers from a sugar feeder in a hightunnel experiment as well as from melon flowers and knapweed in field experiments.Electroantennogram recordings were conducted to verify an olfactory response of the bees to the tested compounds. Pheromonal compounds were readily detected by bee antennae; whereas, the HCAs did not elicit significant responses in the bee antennae. These data suggest that bee pheromonal compounds, as well as HCAs, may serve as candidates for the further investigation as repellents to protect bees from unintentional pesticide exposures. Chemical Manipulation of Honey Bee Behavior Nicholas R. Larson GENERAL AUDIENCE ABSTRACTThe loss of managed honey bee colonies, resulting from their unintentional exposure to pesticides, is a topic of concern for the agricultural and apicultural industry. Current methods for reducing pesticide exposures to bees involve the application of pesticides before crop bloom or in the evening when foraging bees are less likely to be exposed to these applications. There is an urgent need for additional protection procedures to reduce the annual losses of managed bee colonies.One method for protecting these pollinators is use of chemical deterrents to reduce the interaction times of foraging bees with pesticide-treated crops. Insect repellents (IRs) primarily have been used for biting and blood-feeding arthropod pests. However, it has been shown that bees can be repelled from pesticide-treated crops using DEET and bee pheromonal compounds. Here, I report the toxicological and deterrent effects of bee pheromonal compounds as well as the deterrent effects of heterocyclic amines (HCAs)...

show abstract

Prochloraz and coumaphos induce different gene expression patterns in three developmental stages of the Carniolan honey bee (Apis mellifera carnica Pollmann)

Cited by 51 publications

References 48 publications

Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor

Expression and Activity of Lysozyme in Apis Mellifera Carnica Brood Infested with Varroa Destructor

Looking for the causes of and solutions to the issue of honey bee colony losses

Chemical manipulation of honey bee behavior

Contact Info

Product

Resources

About