Procoagulant Activity of Red Blood Cell-Derived Microvesicles during Red Cell Storage

Tayer, Akbar Hashemi; Amirizadeh, Naser; Ahmadinejad, Minoo; Nikougoftar, Mahin; Deyhim, Mohammad Reza; Zolfaghari, Sima

doi:10.1159/000494367

Cited by 47 publications

(40 citation statements)

References 29 publications

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“…A significant increase in the concentration of RBCEVs following storage at 4°C was reported by multiple studies [ 84 , 130 , 131 ]. Further, RBCEVs accumulated during refrigerated storage were demonstrated to express PS on their surface [ 130 , 132 ]. The procoagulant activity of RBCEVs secreted from stored RBCs is suggested by results that demonstrate significantly decreased clotting time, enhanced procoagulant activity [ 130 ], and increased TG [ 132 , 133 ].…”

Section: Biological Roles Of Rbcevsmentioning

confidence: 82%

“…RBCs stored ex-vivo under blood banking conditions intended for transfusion undergo several changes including loss of membrane and cell volume through shedding of RBCEVs [ 84 , 130 ]. A significant increase in the concentration of RBCEVs following storage at 4°C was reported by multiple studies [ 84 , 130 , 131 ]. Further, RBCEVs accumulated during refrigerated storage were demonstrated to express PS on their surface [ 130 , 132 ].…”

Section: Biological Roles Of Rbcevsmentioning

confidence: 99%

“…Further, RBCEVs accumulated during refrigerated storage were demonstrated to express PS on their surface [ 130 , 132 ]. The procoagulant activity of RBCEVs secreted from stored RBCs is suggested by results that demonstrate significantly decreased clotting time, enhanced procoagulant activity [ 130 ], and increased TG [ 132 , 133 ]. Ex vivo storage of RBCs for transfusion may lead to the accumulation of cell-free Hb containing RBCEVs [ 108 , 109 ].…”

Section: Biological Roles Of Rbcevsmentioning

confidence: 99%

See 2 more Smart Citations

Extracellular Vesicles from Red Blood Cells and Their Evolving Roles in Health, Coagulopathy and Therapy

Thangaraju

Neerukonda

Katneni

et al. 2020

IJMS

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Red blood cells (RBCs) release extracellular vesicles (EVs) including both endosome-derived exosomes and plasma-membrane-derived microvesicles (MVs). RBC-derived EVs (RBCEVs) are secreted during erythropoiesis, physiological cellular aging, disease conditions, and in response to environmental stressors. RBCEVs are enriched in various bioactive molecules that facilitate cell to cell communication and can act as markers of disease. RBCEVs contribute towards physiological adaptive responses to hypoxia as well as pathophysiological progression of diabetes and genetic non-malignant hematologic disease. Moreover, a considerable number of studies focus on the role of EVs from stored RBCs and have evaluated post transfusion consequences associated with their exposure. Interestingly, RBCEVs are important contributors toward coagulopathy in hematological disorders, thus representing a unique evolving area of study that can provide insights into molecular mechanisms that contribute toward dysregulated hemostasis associated with several disease conditions. Relevant work to this point provides a foundation on which to build further studies focused on unraveling the potential roles of RBCEVs in health and disease. In this review, we provide an analysis and summary of RBCEVs biogenesis, composition, and their biological function with a special emphasis on RBCEV pathophysiological contribution to coagulopathy. Further, we consider potential therapeutic applications of RBCEVs.

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Section: Biological Roles Of Rbcevsmentioning

confidence: 82%

Section: Biological Roles Of Rbcevsmentioning

confidence: 99%

Section: Biological Roles Of Rbcevsmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular Vesicles from Red Blood Cells and Their Evolving Roles in Health, Coagulopathy and Therapy

Thangaraju

Neerukonda

Katneni

et al. 2020

IJMS

View full text Add to dashboard Cite

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“…This should be easily achieved by employing fresh platelet units for substitution therapies [ 38 ]. Similar concern applies to stored erythrocytes that undergo structural and biochemical alterations and release procoagulant ExMVs [ 69 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular microvesicles/exosomes: discovery, disbelief, acceptance, and the future?

2020

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There are concepts in science that need time to overcome initial disbelief before finally arriving at the moment when they are embraced by the research community. One of these concepts is the biological meaning of the small, spheroidal vesicles released from cells, which are described in the literature as microparticles, microvesicles, or exosomes. In the beginning, this research was difficult, as it was hard to distinguish these small vesicles from cell debris or apoptotic bodies. However, they may represent the first language of cell–cell communication, which existed before a more specific intercellular cross-talk between ligands and receptors emerged during evolution. In this review article, we will use the term “extracellular microvesicles” (ExMVs) to refer to these small spheroidal blebs of different sizes surrounded by a lipid layer of membrane. We have accepted an invitation from the Editor-in-Chief to write this review in observance of the 20th anniversary of the 2001 ASH Meeting when our team demonstrated that, by horizontal transfer of several bioactive molecules, including mRNA species and proteins, ExMVs harvested from embryonic stem cells could modify hematopoietic stem/progenitor cells and expand them ex vivo. Interestingly, the result that moved ExMV research forward was published first in 2005 in Leukemia, having been previously rejected by other major scientific journals out of simple disbelief. Therefore, the best judge of a new concept is the passage of time, although the speed of its adoption is aided by perseverance and confidence in one’s own data. In this perspective article, we will provide a brief update on the current status of, hopes for, and likely future of ExMV research as well as therapeutic and diagnostic applications, with a special emphasis on hematopoiesis.

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“…This prevents translocation of phosphatidylserine (PS) and phosphatidylethanolamine (PE) to the inner side of the membrane, thus disturbing the phospholipid composition of the plasma membrane [ 68 ]. As a result, MVs bud from the cell membrane with both PS and PE present on the surface [ 78 , 81 ].…”

Section: The Biogenesis Of Mvsmentioning

confidence: 99%

Membrane Microvesicles as Potential Vaccine Candidates

Shkair

Garanina

Stott

et al. 2021

IJMS

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The prevention and control of infectious diseases is crucial to the maintenance and protection of social and public healthcare. The global impact of SARS-CoV-2 has demonstrated how outbreaks of emerging and re-emerging infections can lead to pandemics of significant public health and socio-economic burden. Vaccination is one of the most effective approaches to protect against infectious diseases, and to date, multiple vaccines have been successfully used to protect against and eradicate both viral and bacterial pathogens. The main criterion of vaccine efficacy is the induction of specific humoral and cellular immune responses, and it is well established that immunogenicity depends on the type of vaccine as well as the route of delivery. In addition, antigen delivery to immune organs and the site of injection can potentiate efficacy of the vaccine. In light of this, microvesicles have been suggested as potential vehicles for antigen delivery as they can carry various immunogenic molecules including proteins, nucleic acids and polysaccharides directly to target cells. In this review, we focus on the mechanisms of microvesicle biogenesis and the role of microvesicles in infectious diseases. Further, we discuss the application of microvesicles as a novel and effective vaccine delivery system.

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Procoagulant Activity of Red Blood Cell-Derived Microvesicles during Red Cell Storage

Cited by 47 publications

References 29 publications

Extracellular Vesicles from Red Blood Cells and Their Evolving Roles in Health, Coagulopathy and Therapy

Extracellular Vesicles from Red Blood Cells and Their Evolving Roles in Health, Coagulopathy and Therapy

Extracellular microvesicles/exosomes: discovery, disbelief, acceptance, and the future?

Membrane Microvesicles as Potential Vaccine Candidates

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