Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen

Abstract

AIM: To investigate the role of procollagen C-proteinase enhancer 1 (PCPE1) in retinal angiogenesis and relevant mechanisms. METHODS: The Pcolce1-knockout (KO) mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo. Pcolce1 siRNA were designed, cell count kit 8 (CCK8) assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells (hRMECs) in vitro. Mouse embryo fibroblasts (MEF) cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen cleavage. RESULTS: In vivo studies showed that the retinal vascular density of Pcolce1-/- mice was significantly lower than that of the control group. Furthermore, silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of hRMECs in vitro. Additionally, much more pro-collagen was found in Pcolce1-/- MEF cells, compared to wild type MEF cells. CONCLUSION: PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen, which may provide a potential therapeutic target of retinal vascular disease.

Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism

“…The mechanism of retinal angiogenesis is very complex, and retinal neovascularization plays a crucial role in the pathological progression of diabetic eye disease. However, there is a lack of relevant research [8,[16][17] . Many studies have shown that there is a close relationship between HDAC functions and diabetes.…”

“…Int J Ophthalmol, Vol. 16 Histone deacetylases (HDACs) plays a crucial role in regulating the development of various retinal cells, including retinal progenitor cells, photoreceptors, bipolar cells, ganglion cells and Müller glial cells [11] . HDACs IIa (HDAC4/5/7/9) regulate many aspects of cell biology, including proliferation, differentiation, autophagy, apoptosis, migration and angiogenesis [12][13] .…”