The study had the broad objective of determining the effect of a variety of procurement practices on the organizational performance of tertiary institutions in Bayelsa State. Survey research design was adopted whereby primary data were obtained from 165 procurement and store/warehouse officers and administrative officers of the various institutions using a 5-point Likert scale structured questionnaire. Data were analyzed using multiples regression technique with the help of Statistical Package for the Social Sciences (SPSS 23). The findings of the study revealed that tendering, bidding, E-procurement, and sustainable procurement practice have a significant effect on the performance of tertiary institutions in Bayelsa State. Hence, the study concluded that procurement practices have a significant effect on the performance of tertiary institutions in Bayelsa State. The researchers recommended that there should be adequate measures put in place to ensure that the best supplier is contracted by the organization as this may enhance performance. There should be adequate measures put in place to ensure that the supplier with the best tender is contracted by the organization as this will serve time and reduce cost. Finally, the study recommended that sustainable procurement practice should be inculcated in the organization, as this will help protect and preserve its environment.